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UNC45B Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422079

MBS422079 is an antibody targeting UNC45B. Also known as Goat anti-UNC45B Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
UNC45B, Polyclonal Antibody
Full Product Name
Goat anti-UNC45B Antibody
Product Gene Name
Product Synonym Names
unc-45 homolog B (C. elegans); CMYA4; FLJ38610; MGC119540; MGC119541; UNC45; cardiomyopathy associated 4; striated muscle UNC45; unc-45 homolog B (C. elegans) antibody; CMYA4 antibody; FLJ38610 antibody; MGC119540 antibody; MGC119541 antibody; UNC45 antibody; cardiomyopathy associated 4 antibody; striated muscle UNC45 antibody
Immunogen Sequence
RSDKLRQKIF KER
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
UNC45B peptide (MBS425758) is used for blocking the activity of UNC45B antibody (MBS422079)
Molecular Weight
103,605 Da
OMIM
611220
Immunogen
Peptide with sequence C-RSDKLRQKIFKER, from the internal region of the protein sequence according to NP_775259.1; NP_001028748.1.
Epitope
Internal region
Note
This antibody is expected to recognise both reported isoforms (NP_775259.1and NP_001028748.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-UNC45B antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-UNC45B antibody
Peptide ELISA: Antibody detection limit dilution 1: 2000.
Western Blot: Approx 90kDa band observed in Human Skeletal Muscle lysates (calculated MW of 104kDa according to NP_775259.1). Recommended concentration: 0.3-1ug/ml.

Western Blot (WB) of anti-UNC45B antibody

(0.3ug/ml) staining of Human Skeletal Muscle lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-UNC45B Polyclonal Antibody – Cat# MBS422079

Other Notes

Small volumes of anti-UNC45B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for UNC45B. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
protein unc-45 homolog B isoform 1
NCBI Official Synonym Full Names
unc-45 myosin chaperone B
NCBI Official Symbol
UNC45B
NCBI Official Synonym Symbols
CMYA4; UNC45; CTRCT43; SMUNC45; UNC-45B
UniProt Protein Name
Protein unc-45 homolog B
UniProt Synonym Protein Names
SMUNC45
Protein Family
UniProt Gene Name
UNC45B
UniProt Synonym Gene Names
CMYA4; UNC45; Unc-45B
UniProt Entry Name
UN45B_HUMAN
NCBI Protein Information
protein unc-45 homolog B

NCBI Summary for UNC45B

This gene encodes a co-chaperone required for folding and accumulation of type II myosins. The protein consists of three tetratricopeptide repeat motifs at the N-terminus that form a complex with heat shock protein 90, a central region of unknown function that is conserved in all Unc-45 proteins, and a C-terminal Unc-45/Cro1/She4 domain. The protein is expressed at high levels in striated muscle, where its muscle myosin chaperone activity is dependent on heat shock protein 90 acting as a co-chaperone. A missense mutation in this gene has been associated with cataract development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
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UniProt Comments for UNC45B

UNC45B: Acts as a co-chaperone for HSP90 and is required for proper folding of the myosin motor domain. Plays a role in sarcomere formation during muscle cell development. 3 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: cytoplasm; cytosol

Molecular Function: Hsp90 protein binding; protein binding

Biological Process: cell differentiation; lens development in camera-type eye; muscle development

Disease: Cataract 43
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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