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NAT1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422200

MBS422200 is an antibody targeting NAT1. Also known as Goat anti-NAT1 Antibody. Reactive in Expected from sequence similarity: Human. Host: Goat. More details

$305 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
NAT1, Polyclonal Antibody
Full Product Name
Goat anti-NAT1 Antibody
Product Gene Name
Product Synonym Names
Goat Anti-NAT1 (aa272-286) Antibody; NAT1; N-acetyltransferase 1 (arylamine N-acetyltransferase); AAC1; NATI; N-acetyltransferase 1; arylamide acetylase 1 (N-acetyltransferase 1); arylamine N-acetyltransferase 1; NAT1 antibody; N-acetyltransferase 1 (arylamine N-acetyltransferase) antibody; AAC1 antibody; NATI antibody; N-acetyltransferase 1 antibody; arylamide acetylase 1 (N-acetyltransferase 1) antibody; arylamine N-acetyltransferase 1 antibody
Immunogen Sequence
HSDLLEDSKY RKIY
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
NAT1 peptide (MBS425555) is used for blocking the activity of NAT1 antibody (MBS422200)
Molecular Weight
33,899 Da
OMIM
108345
Immunogen
Peptide with sequence C-HSDLLEDSKYRKIY, from the internal region of the protein sequence according to NP_000653.3.
Epitope
Internal region
Note
This antibody is not expected to cross-react with Human NAT2.
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-NAT1 antibody
Peptide ELISA (EIA)
Application Notes for anti-NAT1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Preliminary experiments gave an approx.50kDa band in Human Liver lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 33.9kDa according to NP_000653.3. The 50kDa band was successfully blocked by incubation with the immunizing peptide.

Other Notes

Small volumes of anti-NAT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NAT1. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
arylamine N-acetyltransferase 1 isoform a
NCBI Official Synonym Full Names
N-acetyltransferase 1 (arylamine N-acetyltransferase)
NCBI Official Symbol
NAT1
NCBI Official Synonym Symbols
AAC1; MNAT; NATI; NAT-1
UniProt Protein Name
Arylamine N-acetyltransferase 1
UniProt Synonym Protein Names
Arylamide acetylase 1; Monomorphic arylamine N-acetyltransferase; MNAT; N-acetyltransferase type 1; NAT-1
UniProt Gene Name
NAT1
UniProt Synonym Gene Names
AAC1; MNAT; NAT-1
UniProt Entry Name
ARY1_HUMAN
NCBI Protein Information
arylamine N-acetyltransferase 1

NCBI Summary for NAT1

This gene is one of two arylamine N-acetyltransferase (NAT) genes in the human genome, and is orthologous to the mouse and rat Nat2 genes. The enzyme encoded by this gene catalyzes the transfer of an acetyl group from acetyl-CoA to various arylamine and hydrazine substrates. This enzyme helps metabolize drugs and other xenobiotics, and functions in folate catabolism. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2011]
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UniProt Comments for NAT1

NAT1: Participates in the detoxification of a plethora of hydrazine and arylamine drugs. Catalyzes the N- or O-acetylation of various arylamine and heterocyclic amine substrates and is able to bioactivate several known carcinogens. Belongs to the arylamine N-acetyltransferase family.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; Secondary Metabolites Metabolism - caffeine; EC 2.3.1.5; Acetyltransferase

Chromosomal Location of Human Ortholog: 8p22

Cellular Component: cytosol

Molecular Function: arylamine N-acetyltransferase activity

Biological Process: xenobiotic metabolic process
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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