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CDH11 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422381

MBS422381 is an antibody targeting CDH11. Also known as Goat anti-CDH11 (aa 497-509) Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CDH11, Polyclonal Antibody
Full Product Name
Goat anti-CDH11 (aa 497-509) Antibody
Product Gene Name
Product Synonym Names
Goat Anti-CDH11 (aa 26-37) Antibody; CDH11; cadherin 11, type 2, OB-cadherin (osteoblast); CAD11; CDHOB; OB; OSF-4; OB-cadherin; cadherin 11, type 2; cadherin-11; osteoblast cadherin; CAD11 antibody; cadherin 11; type 2 antibody; cadherin 11; type 2; OB-cadherin (osteoblast) antibody; cadherin-11 antibody; CDHOB antibody; OB antibody; OB-cadherin antibody; OSF-4 antibody; osteoblast cadherin antibody; CDH11 antibody; CDH11 (aa 497-509)
Immunogen Sequence
CESDQTKPLS NQP
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
CDH11 peptide (MBS427317) is used for blocking the activity of CDH11 antibody (MBS422381)
Molecular Weight
76,457 Da
OMIM
600023
Immunogen
Peptide with sequence CESDQTKPLSNQP, from the internal region of the protein sequence according to NP_001788.2.
Epitope
Internal region
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-CDH11 antibody
Peptide ELISA (EIA), Western Blot (WB-Trf)
Application Notes for anti-CDH11 antibody
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: In transfected HEK293 transiently expressing full-length Human CDH11 (myc and DYKDDDDK tagged), a band of approx.130kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5ug/ml.

Western Blot (WB) of anti-CDH11 antibody

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human CDH11 with C-terminal MYC tag probed with (1ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Goat Anti-CDH11 Polyclonal Antibody – Cat# MBS422381

Other Notes

Small volumes of anti-CDH11 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CDH11. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
cadherin-11 isoform 1 preproprotein
NCBI Official Synonym Full Names
cadherin 11
NCBI Official Symbol
CDH11
NCBI Official Synonym Symbols
OB; CAD11; CDHOB; OSF-4
UniProt Protein Name
Cadherin-11
UniProt Synonym Protein Names
OSF-4; Osteoblast cadherin; OB-cadherin
Protein Family
UniProt Gene Name
CDH11
UniProt Synonym Gene Names
OB-cadherin
UniProt Entry Name
CAD11_HUMAN
NCBI Protein Information
cadherin-11

NCBI Summary for CDH11

This gene encodes a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins. Expression of this particular cadherin in osteoblastic cell lines, and its upregulation during differentiation, suggests a specific function in bone development and maintenance. [provided by RefSeq, Jul 2008]
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UniProt Comments for CDH11

CDH11: Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. Interacts with PCDH8. Expressed mainly in brain but also found in other tissues. Expressed in neuroblasts. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 16q21

Cellular Component: cytoplasm; integral to membrane; plasma membrane

Molecular Function: calcium ion binding

Biological Process: cell adhesion; corticospinal tract morphogenesis; homophilic cell adhesion; ossification; skeletal development
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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