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POU2F1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422485

MBS422485 is an antibody targeting POU2F1. Also known as Goat anti-POU2F1/OCT1 Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
POU2F1/OCT1, Polyclonal Antibody
Full Product Name
Goat anti-POU2F1/OCT1 Antibody
Product Gene Name
anti-POU2F1 antibody
Product Synonym Gene Name
OCT1
Product Synonym Names
POU2F1; POU class 2 homeobox 1; OCT1; OTF1; OTTHUMP00000032348; Octamer-binding transcription factor-1; POU domain, class 2, transcription factor 1; OCT1 antibody; Octamer-binding transcription factor-1 antibody; OTF1 antibody; OTTHUMP00000032348 antibody; POU class 2 homeobox 1 antibody; POU domain; class 2; transcription factor 1 antibody; POU2F1 antibody; POU2F1 / OCT1
Immunogen Sequence
NVQSKSNEES GDSQQ
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
POU2F1 / OCT1 peptide (MBS427744) is used for blocking the activity of POU2F1/OCT1 antibody (MBS422485)
Molecular Weight
72,082 Da
OMIM
164175
Immunogen
Peptide with sequence C-NVQSKSNEESGDSQQ, from the internal region (near N Terminus) of the protein sequence according to NP_002688.2.
Epitope
Internal region (near N Terminus)
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-POU2F1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-POU2F1 antibody
Peptide ELISA: Antibody detection limit dilution 1: 4000.
Western Blot: Approx 100kDa band observed in nuclear lysates of cell line HeLa (calculated MW of 76.5kDa according to NP_002688.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 0.5-2ug/ml.

Western Blot (WB) of anti-POU2F1 antibody

(0.5ug/ml) staining of nuclear HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-POU2F1 Polyclonal Antibody – Cat# MBS422485

Other Notes

Small volumes of anti-POU2F1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for POU2F1. It may not necessarily be applicable to this product.

NCBI GI #
42476164
NCBI GeneID
5451
NCBI Accession #
NP_002688.2
UniProt Secondary Accession #
Q5TBT7; Q6PK46; Q8NEU9; Q9BPV1; B1AL91; B1AL93; B4E029; J3KP77
UniProt Related Accession #
P14859
GenBank Nucleotide #
NM_002697.2
NCBI Official Full Name
POU domain, class 2, transcription factor 1
NCBI Official Synonym Full Names
POU class 2 homeobox 1
NCBI Official Symbol
POU2F1
NCBI Official Synonym Symbols
OCT1; OTF1; oct-1B
UniProt Protein Name
POU domain, class 2, transcription factor 1
UniProt Synonym Protein Names
NF-A1; Octamer-binding protein 1; Oct-1; Octamer-binding transcription factor 1; OTF-1
Protein Family
POU domain, class 2, transcription factor
UniProt Gene Name
POU2F1
UniProt Synonym Gene Names
OCT1; OTF1; Oct-1; OTF-1
UniProt Entry Name
PO2F1_HUMAN
NCBI Protein Information
POU domain, class 2, transcription factor 1

NCBI Summary for POU2F1

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al., 1993 [PubMed 8314572]). Members of this family contain the POU domain, a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM, Jul 2010]
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UniProt Comments for POU2F1

Oct1: a ubiquitous transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR. Phosphorylation apparently inhibits its interaction with DNA. Four splice variant isoforms have been described. Isoform 2 is lymphocyte specific.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q24.2

Cellular Component: endoplasmic reticulum; intracellular membrane-bound organelle; nucleoplasm; nucleus

Molecular Function: protein binding; sequence-specific DNA binding

Biological Process: negative regulation of transcription, DNA-dependent; snRNA transcription from RNA polymerase II promoter
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Research Articles on POU2F1

1. OCT1 siRNA significantly reduced the proliferation rate of SW620 and LoVo cells, inhibited the migration and invasion, and could reverse EMT in these two CRC cells, indicating that OCT1 plays a critical role in Colorectal cancer progression

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-POU2F1 antibody

 Reference Product  PubMed Publications
 HCFC1 antibody  >388 publications with POU2F1 and HCFC1
 SP1 antibody  >42 publications with POU2F1 and SP1
 POU2AF1 antibody  >28 publications with POU2F1 and POU2AF1
 NF1 antibody  >7 publications with POU2F1 and NF1
 SOX2 antibody  >7 publications with POU2F1 and SOX2
 TBP antibody  >4 publications with POU2F1 and TBP
 BRCA1 antibody  >4 publications with POU2F1 and BRCA1
 SNAPC4 antibody  >2 publications with POU2F1 and SNAPC4
 PRKDC antibody  >1 publications with POU2F1 and PRKDC

Pathways associated with anti-POU2F1 antibody

 Products by Pathway  Pathway Diagram
 ATF-2 Transcription Factor Network Pathway antibodies  ATF-2 Transcription Factor Network Pathway Diagram
 Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway antibodies  Calcineurin-regulated NFAT-dependent Transcription In Lymphocytes Pathway Diagram
 Calcium Signaling In The CD4+ TCR Pathway antibodies  Calcium Signaling In The CD4+ TCR Pathway Diagram
 FOXA1 Transcription Factor Network Pathway antibodies  FOXA1 Transcription Factor Network Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Glucocorticoid Receptor Regulatory Network Pathway antibodies  Glucocorticoid Receptor Regulatory Network Pathway Diagram
 RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway antibodies  RNA Polymerase I, RNA Polymerase III, And Mitochondrial Transcription Pathway Diagram
 RNA Polymerase III Abortive And Retractive Initiation Pathway antibodies  RNA Polymerase III Abortive And Retractive Initiation Pathway Diagram
 RNA Polymerase III Transcription Pathway antibodies  RNA Polymerase III Transcription Pathway Diagram
 RNA Polymerase III Transcription Initiation Pathway antibodies  RNA Polymerase III Transcription Initiation Pathway Diagram

Diseases associated with anti-POU2F1 antibody

 Disease Name  Pubmed Publications
 Necrosis Antibodies  >15 publications with POU2F1 and Necrosis
 Inflammation Antibodies  >13 publications with POU2F1 and Inflammation
 Nervous System Diseases Antibodies  >10 publications with POU2F1 and Nervous System Diseases
 Kidney Diseases Antibodies  >7 publications with POU2F1 and Kidney Diseases
 Lung Diseases Antibodies  >6 publications with POU2F1 and Lung Diseases
 Liver Diseases Antibodies  >5 publications with POU2F1 and Liver Diseases
 Brain Diseases Antibodies  >5 publications with POU2F1 and Brain Diseases
 Carcinoma, Hepatocellular Antibodies  >4 publications with POU2F1 and Carcinoma, Hepatocellular
 Liver Neoplasms Antibodies  >3 publications with POU2F1 and Liver Neoplasms
 Cholestasis Antibodies  >3 publications with POU2F1 and Cholestasis

Organs/Tissues associated with anti-POU2F1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >134 publications with POU2F1 and Blood
 Liver Antibodies  >42 publications with POU2F1 and Liver
 Kidney Antibodies  >36 publications with POU2F1 and Kidney
 Brain Antibodies  >33 publications with POU2F1 and Brain
 Embryonic Tissue Antibodies  >18 publications with POU2F1 and Embryonic Tissue
 Vascular Antibodies  >14 publications with POU2F1 and Vascular
 Lung Antibodies  >14 publications with POU2F1 and Lung
 Spleen Antibodies  >12 publications with POU2F1 and Spleen
 Muscle Antibodies  >11 publications with POU2F1 and Muscle
 Thymus Antibodies  >8 publications with POU2F1 and Thymus
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