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SDF4 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422557

MBS422557 is an antibody targeting SDF4. Also known as Goat anti-SDF4 (aa161-175) Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
SDF4, Polyclonal Antibody
Full Product Name
Goat anti-SDF4 (aa161-175) Antibody
Product Gene Name
Product Synonym Names
SDF4; stromal cell derived factor 4; Cab45; 45 kDa calcium-binding protein; OTTHUMP00000001754; SDF-4; calcium binding protein; stromal cell-derived factor 4; SDF4 antibody; stromal cell derived factor 4 antibody; Cab45 antibody; 45 kDa calcium-binding protein antibody; OTTHUMP00000001754 antibody; SDF-4 antibody; calcium binding protein antibody; stromal cell-derived factor 4 antibody; SDF4 (aa161-175)
Immunogen Sequence
EYKVKFLASK GHSEK
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Matching Pairs
Unconjugated Antibody: SDF4 (MBS422557)
Biotin Conjugated Antibody: SDF4 (MBS423749)
Antibody/Peptide Pairs
SDF4 peptide (MBS427443) is used for blocking the activity of SDF4 antibody (MBS422557)
Molecular Weight
39,608 Da
OMIM
614282
Immunogen
Peptide with sequence C-EYKVKFLASKGHSEK, from the internal region of the protein sequence according to NP_057631.1; NP_057260.2.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_057631.1; NP_057260.2).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-SDF4 antibody
Peptide ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-SDF4 antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Immunohistochemistry: In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration, 5-10ug/ml.
Western Blot: Approx 45kDa band observed in lysates of cell line Jurkat (calculated MW of 41.8kDa according to NP_057260.2). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-SDF4 antibody

(0.1ug/ml) staining of Jurkat lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-SDF4 Polyclonal Antibody – Cat# MBS422557

Immunohistochemistry (IHC) of anti-SDF4 antibody

(5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Goat Anti-SDF4 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS422557

Other Notes

Small volumes of anti-SDF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SDF4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
45 kDa calcium-binding protein isoform 1
NCBI Official Synonym Full Names
stromal cell derived factor 4
NCBI Official Symbol
SDF4
NCBI Official Synonym Symbols
Cab45; SDF-4
UniProt Protein Name
45 kDa calcium-binding protein
UniProt Synonym Protein Names
Stromal cell-derived factor 4; SDF-4
UniProt Gene Name
SDF4
UniProt Synonym Gene Names
CAB45; Cab45; SDF-4
UniProt Entry Name
CAB45_HUMAN
NCBI Protein Information
45 kDa calcium-binding protein

NCBI Summary for SDF4

This gene encodes a stromal cell derived factor that is a member of the CREC protein family. The encoded protein contains six EF-hand motifs and calcium-binding motifs. This protein localizes to the Golgi lumen and may be involved in regulating calcium dependent cellular activities. [provided by RefSeq, Sep 2011]
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UniProt Comments for SDF4

SDF4: May regulate calcium-dependent activities in the endoplasmic reticulum lumen or post-ER compartment. Belongs to the CREC family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 1p36.33

Cellular Component: cytoplasm; Golgi apparatus; Golgi lumen; late endosome; membrane; plasma membrane

Molecular Function: calcium ion binding; identical protein binding; protein binding

Biological Process: calcium ion-dependent exocytosis; cerebellum development; fat cell differentiation; response to ethanol; UV protection
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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