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MST2/STK3 (STK3) Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422670

MBS422670 is an antibody targeting STK3. Also known as Goat anti-MST2/STK3 Antibody. Reactive in Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
MST2/STK3, Polyclonal Antibody
Full Product Name
Goat anti-MST2/STK3 Antibody
Product Gene Name
anti-STK3 antibody
Product Synonym Gene Name
MST2
Product Synonym Names
STK3; serine/threonine kinase 3 (STE20 homolog, yeast); FLJ90748; KRS1; MST2; serine/threonine kinase 3 (Ste20, yeast homolog); FLJ90748 antibody; KRS1 antibody; MST2 antibody; serine/threonine kinase 3 (STE20 homolog; yeast) antibody; serine/threonine kinase 3 (Ste20; yeast homolog) antibody; STK3 antibody; MST2 / STK3
Immunogen Sequence
DFKNKSHENC NQN
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human, Mouse; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
MST2 / STK3 peptide (MBS427463) is used for blocking the activity of MST2/STK3 antibody (MBS422670)
Molecular Weight
59,462 Da
OMIM
605030
Immunogen
Peptide with sequence DFKNKSHENCNQN, from the internal region of the protein sequence according to NP_006272.2.
Epitope
Internal region
Note
This antibody is not expected to cross-react to STK4
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-STK3 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-STK3 antibody
Peptide ELISA: Antibody detection limit dilution 1: 16000.
Western Blot: Approx 65kDa band observed in lysates of cell lines A431 and NIH3T3 (calculated MW of 56.3kDa according to Human NP_006272.2 and 56.8kDa according to Mouse NP_062609.2). The observed molecular weight corresponds to earlier findings with different antibodies from other commercial sources. Recommended concentration: 1-3ug/ml.

Western Blot (WB) of anti-STK3 antibody

(1ug/ml) staining of NIH3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-STK3 Polyclonal Antibody – Cat# MBS422670

Other Notes

Small volumes of anti-STK3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for STK3. It may not necessarily be applicable to this product.

NCBI GI #
103471999
NCBI GeneID
6788
NCBI Accession #
NP_006272.2
UniProt Secondary Accession #
Q15445; Q15801; Q96FM6; A8K722; B3KYA7
UniProt Related Accession #
Q13188
GenBank Nucleotide #
NM_006281.3
NCBI Official Full Name
serine/threonine-protein kinase 3 isoform 1
NCBI Official Synonym Full Names
serine/threonine kinase 3
NCBI Official Symbol
STK3
NCBI Official Synonym Symbols
KRS1; MST2
UniProt Protein Name
Serine/threonine-protein kinase 3
UniProt Synonym Protein Names
Mammalian STE20-like protein kinase 2; MST-2
Protein Family
Serine/threonine-protein kinase
UniProt Gene Name
STK3
UniProt Synonym Gene Names
KRS1; MST2; MST-2; MST2/N; MST2/C
UniProt Entry Name
STK3_HUMAN
NCBI Protein Information
serine/threonine-protein kinase 3

NCBI Summary for STK3

This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]
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UniProt Comments for STK3

MST2: a protein kinase of the STE20 family. Activated by apoptotic signals as well as other stress conditions. Full activation requires both phosphorylation and caspase-mediated cleavage. Following cleavage by caspase-3, undergoes irreversible autophosphorylation. Full-length is mainly unphosphorylated in the rat thymus and cultured cells, whereas caspase-3-cleaved MST2 in apoptotic cells is highly phosphorylated.

Protein type: EC 2.7.11.1; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); Autophagy; Kinase, protein; STE group; STE20 family; MST subfamily

Chromosomal Location of Human Ortholog: 8q22.2

Cellular Component: cytoplasm; cytosol; nucleus; protein complex

Molecular Function: ATP binding; magnesium ion binding; protein binding; protein dimerization activity; protein kinase activity; protein serine/threonine kinase activator activity; protein serine/threonine kinase activity; receptor signaling protein serine/threonine kinase activity

Biological Process: apoptosis; central nervous system development; negative regulation of cell proliferation; negative regulation of organ growth; neural tube formation; positive regulation of apoptosis; positive regulation of fat cell differentiation; positive regulation of JNK cascade; positive regulation of protein binding; positive regulation of protein kinase B signaling cascade; positive regulation of transcription factor activity; protein amino acid phosphorylation; protein stabilization; signal transduction
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Research Articles on STK3

1. The MST1/2-SAV1 complex, a core component of the Hippo pathway, promotes ciliogenesis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-STK3 antibody

 Reference Product  PubMed Publications
 LATS1 antibody  >28 publications with STK3 and LATS1
 SAV1 antibody  >14 publications with STK3 and SAV1
 MOB1B antibody  >6 publications with STK3 and MOB1B
 SLMAP antibody  >1 publications with STK3 and SLMAP

Pathways associated with anti-STK3 antibody

 Products by Pathway  Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 Hippo Signaling Pathway antibodies  Hippo Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 MAPK Signaling Pathway antibodies  MAPK Signaling Pathway Diagram
 Signal Transduction Pathway antibodies  Signal Transduction Pathway Diagram
 Signaling By Hippo Pathway antibodies  Signaling By Hippo Pathway Diagram

Diseases associated with anti-STK3 antibody

 Disease Name  Pubmed Publications
 Adenocarcinoma Antibodies  >7 publications with STK3 and Adenocarcinoma
 Breast Neoplasms Antibodies  >6 publications with STK3 and Breast Neoplasms
 Liver Neoplasms Antibodies  >5 publications with STK3 and Liver Neoplasms
 Liver Diseases Antibodies  >4 publications with STK3 and Liver Diseases
 Disease Models, Animal Antibodies  >4 publications with STK3 and Disease Models, Animal
 Carcinoma, Hepatocellular Antibodies  >4 publications with STK3 and Carcinoma, Hepatocellular
 Cell Transformation, Neoplastic Antibodies  >4 publications with STK3 and Cell Transformation, Neoplastic
 Hypertrophy Antibodies  >2 publications with STK3 and Hypertrophy
 Fibrosis Antibodies  >2 publications with STK3 and Fibrosis
 Necrosis Antibodies  >2 publications with STK3 and Necrosis

Organs/Tissues associated with anti-STK3 antibody

 Organ/Tissue Name  Pubmed Publications
 Liver Antibodies  >8 publications with STK3 and Liver
 Lung Antibodies  >8 publications with STK3 and Lung
 Kidney Antibodies  >6 publications with STK3 and Kidney
 Brain Antibodies  >6 publications with STK3 and Brain
 Blood Antibodies  >5 publications with STK3 and Blood
 Nerve Antibodies  >4 publications with STK3 and Nerve
 Heart Antibodies  >3 publications with STK3 and Heart
 Thyroid Antibodies  >2 publications with STK3 and Thyroid
 Prostate Antibodies  >2 publications with STK3 and Prostate
 Embryonic Tissue Antibodies  >2 publications with STK3 and Embryonic Tissue
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