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NRAP Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS422675

MBS422675 is an antibody targeting NRAP. Also known as Goat anti-NRAP Antibody. Reactive in Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig. Host: Goat. More details

$305 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
NRAP, Polyclonal Antibody
Full Product Name
Goat anti-NRAP Antibody
Product Gene Name
Product Synonym Names
NRAP; nebulin-related anchoring protein; nebulin-related anchoring protein antibody; NRAP antibody
Immunogen Sequence
DFAKSRSQFH
Clonality
Polyclonal
Host
Goat
Species Reactivity
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration
100ug specific antibody in 200ul (lot specific)
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
NRAP peptide (MBS428210) is used for blocking the activity of NRAP antibody (MBS422675)
Molecular Weight
192,997 Da
OMIM
602873
Immunogen
Peptide with sequence C-DFAKSRSQFH, from the internal region of the protein sequence according to NP_006166.3; NP_932326.2.
Epitope
Internal region
Note
This antibody is expected to recognize both reported isoforms (NP_006166.3; NP_932326.2).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-NRAP antibody
Peptide ELISA (EIA)
Application Notes for anti-NRAP antibody
Peptide ELISA: Antibody detection limit dilution 1: 32000.
Western Blot: Preliminary experiments gave an approx 100kDa band in Human, Mouse and Rat Heart lysates after 1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 193kDa according to NP_006166.3. The 100kDa band was successfully blocked by incubation with the immunizing peptide.

Other Notes

Small volumes of anti-NRAP antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NRAP. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
nebulin-related-anchoring protein isoform 1
NCBI Official Synonym Full Names
nebulin related anchoring protein
NCBI Official Symbol
NRAP
NCBI Official Synonym Symbols
N-RAP
UniProt Protein Name
Nebulin-related-anchoring protein
UniProt Gene Name
NRAP
UniProt Synonym Gene Names
N-RAP
UniProt Entry Name
NRAP_HUMAN
NCBI Protein Information
nebulin-related-anchoring protein

UniProt Comments for NRAP

NRAP: May be involved in anchoring the terminal actin filaments in the myofibril to the membrane and in transmitting tension from the myofibrils to the extracellular matrix. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 10q24-q26

Cellular Component: fascia adherens; muscle tendon junction

Molecular Function: actin binding; muscle alpha-actinin binding; zinc ion binding
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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