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AMACR Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS423435

MBS423435 is an antibody targeting AMACR. Also known as Goat anti-AMACR (aa312-326) Antibody. Reactive in Tested: Human; Expected from sequence similarity: Human. Host: Goat. More details

$450 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
AMACR, Polyclonal Antibody
Full Product Name
Goat anti-AMACR (aa312-326) Antibody
Product Gene Name
Product Synonym Names
AMACR, alpha-methylacyl-CoA racemase, AMACRD, CBAS4, RACE, RM, 2-methylacyl-CoA racemase
Immunogen Sequence
RGSFITSEEQ DVSPR
Clonality
Polyclonal
Host
Goat
Species Reactivity
Tested: Human; Expected from sequence similarity: Human
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Antibody/Peptide Pairs
AMACR peptide (MBS428004) is used for blocking the activity of AMACR antibody (MBS423435)
Molecular Weight
43,860 Da
OMIM
214950
Immunogen
Peptide with sequence C-RGSFITSEEQDVSPR, from the internal region of the protein sequence according to NP_055139.4; NP_001161067.1.
Note
This antibody is expected to recognize isoform 1 (NP_055139.4) and isoform 3 (NP_001161067.1).
ISO Certification
Manufactured in an ISO 9001:2008 Certified Laboratory.
Applications Tested/Suitable for anti-AMACR antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes for anti-AMACR antibody
Peptide ELISA: Antibody detection limit dilution 1: 64000.
Western Blot: Approx 45kDa band observed in Human Kidney and Liver lysates (calculated MW of 42.4kDa according to NP_055139.4). Recommended concentration: 0.1-0.3ug/ml.

Western Blot (WB) of anti-AMACR antibody

(0.1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Goat Anti-AMACR Polyclonal Antibody – Cat# MBS423435

Other Notes

Small volumes of anti-AMACR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for AMACR. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI Related Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-methylacyl-CoA racemase isoform 1
NCBI Official Synonym Full Names
alpha-methylacyl-CoA racemase
NCBI Official Symbol
AMACR
NCBI Official Synonym Symbols
RM; RACE; CBAS4; AMACRD
UniProt Protein Name
Alpha-methylacyl-CoA racemase
UniProt Synonym Protein Names
2-methylacyl-CoA racemase
UniProt Gene Name
AMACR
UniProt Entry Name
AMACR_HUMAN
NCBI Protein Information
alpha-methylacyl-CoA racemase

NCBI Summary for AMACR

This gene encodes a racemase. The encoded enzyme interconverts pristanoyl-CoA and C27-bile acylCoAs between their (R)- and (S)-stereoisomers. The conversion to the (S)-stereoisomers is necessary for degradation of these substrates by peroxisomal beta-oxidation. Encoded proteins from this locus localize to both mitochondria and peroxisomes. Mutations in this gene may be associated with adult-onset sensorimotor neuropathy, pigmentary retinopathy, and adrenomyeloneuropathy due to defects in bile acid synthesis. Alternatively spliced transcript variants have been described. Read-through transcription also exists between this gene and the upstream neighboring C1QTNF3 (C1q and tumor necrosis factor related protein 3) gene. [provided by RefSeq, Mar 2011]
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UniProt Comments for AMACR

AMACR: Racemization of 2-methyl-branched fatty acid CoA esters. Responsible for the conversion of pristanoyl-CoA and C27-bile acyl-CoAs to their (S)-stereoisomers. Belongs to the CaiB/BaiF CoA-transferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - primary bile acid biosynthesis; Isomerase; Mitochondrial; EC 5.1.99.4

Chromosomal Location of Human Ortholog: 5p13

Cellular Component: cytoplasm; mitochondrion; peroxisomal matrix; peroxisome

Molecular Function: alpha-methylacyl-CoA racemase activity; receptor binding

Biological Process: bile acid biosynthetic process; bile acid metabolic process; fatty acid beta-oxidation using acyl-CoA oxidase

Disease: Alpha-methylacyl-coa Racemase Deficiency; Bile Acid Synthesis Defect, Congenital, 4
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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