Cell Lysis Buffer Reagent

2-8 degree C.

1. Lysis of bacterial cells
Collecting bacterial cells by centrifugation ((10,000 x g, 0 degree C, 15 min). Use about 100 to 10 million cells/mL lysis buffer, leave at room temperature for 15 minutes. And then centrifuge at 2500 rpm for 5 minutes, use the supernatant for the assay.
2. Lysis of plant cells:
Homogenize the leave with the lysis buffer at 200 mg/mL, and then centrifuge at 2500 rpm for 5-10 minutes, use the supernatant for the assay.
3. Lysis of mammalian cells
Simply remove medium from the plates (wells), use about 100 uL lysis buffer per 1-5 million cells (or 100uL/ well in a 96-well cell culture plate), and leave at room temperature for 15 minutes. You can use the cell lysate directly or simply centrifuge at 1500 rpm for 5 minutes, use the supernatant for the assay.
4. Lysis of tissues.
Weight ~20 mg tissue, wash with cold PBS, homogenize with 400 mul of lysis buffer in a micro-centrifuge tube, and then centrifuge at 2500 rpm for 5-10 minutes, use the supernatant for the assay.