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ELF1 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS9600368

Description: Elf-1 Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer. Belongs to… More details

$325 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
ELF1, Polyclonal Antibody
Full Product Name
ELF1 Antibody
Product Gene Name
anti-ELF1 antibody
Product Synonym Names
E74 like factor 1; E74-like factor 1; ELF 1; ELF1; ELF1_HUMAN; Ets domain transcription factor; ETS related transcription factor Elf 1; ETS related transcription factor Elf1; ets-related transcription factor; ETS-related transcription factor Elf-1; mElf 1; p70; Sts1; TRANSCRIPTION FACTOR ELF 1
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Concentration
1mg/ml (lot specific)
Specificity
ELF1 antibody detects endogenous levels of ELF1
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Antibody/Peptide Pairs
ELF1 peptide (MBS9614921) is used for blocking the activity of ELF1 antibody (MBS9600368)
Molecular Weight
Observed: 67 kDa
Predicted: 68 kDa
3D Structure
ModBase 3D Structure for P32519
Supply Chain Verification
Manufactured in a lab with traceable raw materials manufactured on site. Coordinated product portfolio of antibodies, pairs, conjugates, recombinant proteins, and immunoassay materials available, please inquire.
Immunogen
A synthesized peptide derived from human ELF1
Subcellular Location
Nucleus.
Tissue Specificity
In fetal tissues, it is highly expressed in heart, lung liver and kidney, and weakly expressed in brain. In adult, it is highly expressed in pancreas, spleen, thymus and peripheral blood leukocytes, expressed at moderate levels in heart, placenta, lung, liver, skeletal muscle, kidney, prostate, ovary, small intestine and colon, and weakly expressed in brain and testis.
Predicted Cross Reactivity
Horse
Similarity
Horse (80%)
Conjugation
Unconjugated
ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-ELF1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes for anti-ELF1 antibody
WB: 1:500-1:2000
IF/ICC: 1:100-1:500

Immunofluorescence (IF) of anti-ELF1 antibody

MBS9600368 staining CACO-2 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
Rabbit Anti-ELF1 Polyclonal Antibody – Cat# MBS9600368

Immunofluorescene (IF) of anti-ELF1 antibody

MBS9600368 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
Rabbit Anti-ELF1 Polyclonal Antibody, Immunofluorescene (IF) – Cat# MBS9600368

Western Blot (WB) of anti-ELF1 antibody

Western blot analysis on 293 cell lysate using ELF1 Antibody
Rabbit Anti-ELF1 Polyclonal Antibody, Western Blot (WB) – Cat# MBS9600368

Other Notes

Small volumes of anti-ELF1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ELF1 purchase

MBS9600368 is an antibody targeting ELF1. Also known as ELF1 Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for ELF1. It may not necessarily be applicable to this product.

NCBI GI #
223941929
NCBI GeneID
1997
NCBI Accession #
NP_001138825.1
UniProt Primary Accession #
P32519
UniProt Secondary Accession #
Q8N6F6; Q9UDE1; B4E2I5; E9PDQ9
UniProt Related Accession #
P32519
GenBank Nucleotide #
NM_001145353.1
NCBI Official Full Name
ETS-related transcription factor Elf-1 isoform b
NCBI Official Synonym Full Names
E74 like ETS transcription factor 1
NCBI Official Symbol
ELF1
NCBI Official Synonym Symbols
RIA1; EFTUD1
UniProt Protein Name
ETS-related transcription factor Elf-1
UniProt Synonym Protein Names
E74-like factor 1
Protein Family
ETS-related transcription factor
UniProt Gene Name
ELF1
NCBI Protein Information
ETS-related transcription factor Elf-1

NCBI Summary for ELF1

This gene encodes an E26 transformation-specific related transcription factor. The encoded protein is primarily expressed in lymphoid cells and acts as both an enhancer and a repressor to regulate transcription of various genes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2009]
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UniProt Comments for ELF1

Transcription factor that activates the LYN and BLK promoters. Appears to be required for the T-cell-receptor-mediated trans activation of HIV-2 gene expression. Binds specifically to two purine-rich motifs in the HIV-2 enhancer.

Research Articles on ELF1

1. ELF1 binding occurs at both TERT promoter mutations in melanoma in vitro such that increased recruitment of GABP is enabled by the spatial architecture of native and novel ETS motifs in the TERT promoter region.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ELF1 antibody

 Reference Product  PubMed Publications
 SP1 antibody  >4 publications with ELF1 and SP1
 REL antibody  >2 publications with ELF1 and REL
 NFKB1 antibody  >1 publications with ELF1 and NFKB1
 HMGA1 antibody  >1 publications with ELF1 and HMGA1

Pathways associated with anti-ELF1 antibody

 Products by Pathway  Pathway Diagram
 Angiopoietin Receptor Tie2-mediated Signaling Pathway antibodies  Angiopoietin Receptor Tie2-mediated Signaling Pathway Diagram
 IL2 Signaling Events Mediated By STAT5 Pathway antibodies  IL2 Signaling Events Mediated By STAT5 Pathway Diagram
 Regulation Of Retinoblastoma Protein Pathway antibodies  Regulation Of Retinoblastoma Protein Pathway Diagram

Diseases associated with anti-ELF1 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >13 publications with ELF1 and Neoplasms
 Disease Models, Animal Antibodies  >4 publications with ELF1 and Disease Models, Animal
 Lung Diseases Antibodies  >3 publications with ELF1 and Lung Diseases
 Carcinoma, Squamous Cell Antibodies  >2 publications with ELF1 and Carcinoma, Squamous Cell
 Neoplasm Metastasis Antibodies  >2 publications with ELF1 and Neoplasm Metastasis
 Lung Neoplasms Antibodies  >2 publications with ELF1 and Lung Neoplasms
 Brain Diseases Antibodies  >2 publications with ELF1 and Brain Diseases
 Breast Neoplasms Antibodies  >2 publications with ELF1 and Breast Neoplasms
 Hypertension Antibodies  >1 publications with ELF1 and Hypertension
 Ovarian Diseases Antibodies  >1 publications with ELF1 and Ovarian Diseases

Organs/Tissues associated with anti-ELF1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >35 publications with ELF1 and Blood
 Vascular Antibodies  >9 publications with ELF1 and Vascular
 Embryonic Tissue Antibodies  >9 publications with ELF1 and Embryonic Tissue
 Brain Antibodies  >9 publications with ELF1 and Brain
 Lung Antibodies  >6 publications with ELF1 and Lung
 Skin Antibodies  >6 publications with ELF1 and Skin
 Bone Antibodies  >5 publications with ELF1 and Bone
 Liver Antibodies  >5 publications with ELF1 and Liver
 Eye Antibodies  >4 publications with ELF1 and Eye
 Bone Marrow Antibodies  >3 publications with ELF1 and Bone Marrow
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