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Human Nuclear Antigen Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438074

MBS438074 is an antibody targeting Human Nuclear Antigen. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Human Nuclear Antigen, Monoclonal Antibody
Full Product Name
Human Nuclear Antigen
Product Synonym Names
HNA, HuNu
Clonality
Monoclonal
Isotype
IgG1
Clone Number
235-1
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
Specificity
This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Preparation and Storage
Antibody with azide store at 2 to 8°C. Non-hazardous. No MSDS required.
Molecular Weight
70kDa & 80kDa
Immunogen
Nuclei of human myeloid leukemia biopsy cells
Cellular Localization
Nucleus
Positive Control
All human cells. Tissues.
Applications Tested/Suitable for anti-Human Nuclear Antigen antibody
Flow Cytometry (FC), Immunofluorescence (IF)
Application Notes for anti-Human Nuclear Antigen antibody
Flow Cytometry (Flow) 1-2ug/million cells
Immunofluorescence (IF) 1-3ug/ml

Immunofluorescence (IF) of anti-Human Nuclear Antigen antibody

Immunofluorescent staining of paraformaldehyde-fixed HeLa cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-Mouse IgG-CF488 (Green). Counter stained with Phalloidin (red).
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody – Cat# MBS438074

Flow Cytometry (FC/FACS) of anti-Human Nuclear Antigen antibody

Flow Cytometric Analysis of paraformaldehyde-fixed HeLa cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS438074

Immunohistochemistry (IHC) of anti-Human Nuclear Antigen antibody

Acetone-fixed frozen human Tonsil stained with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1).
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438074

SDS-PAGE of anti-Human Nuclear Antigen antibody

SDS-PAGE Analysis Purified Human Nuclear Antigen Mouse Monoclonal Antibody (235-1). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody, SDS-PAGE – Cat# MBS438074

Flow Cytometry (FC) of anti-Human Nuclear Antigen antibody

Flow Cytometric Analysis of PFA-fixed Jurkat cells with Human Nuclear Antigen Mouse Monoclonal Antibody; followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS438074

Flow Cytometry (FC) of anti-Human Nuclear Antigen antibody

Flow Cytometric Analysis of PFA-fixed K562 cells with Human Nuclear Antigen Mouse Monoclonal Antibody; followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS438074

Flow Cytometry (FC) of anti-Human Nuclear Antigen antibody

Flow Cytometric Analysis of PFA-fixed MCF-7 cells with Human Nuclear Antigen Mouse Monoclonal Antibody; followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS438074

Immunofluorescence (IF) of anti-Human Nuclear Antigen antibody

Immunofluorescent staining of PFA-fixed K562 cells with Human Nuclear Antigen Mouse Monoclonal Antibody; followed by goat anti-mouse IgG-CF488 (Green). Counterstained with Phalloidin (red).
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS438074

Immunofluorescence (IF) of anti-Human Nuclear Antigen antibody

Immunofluorescent staining of PFA-fixed MCF-7 cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (Green). Counterstained with Phalloidin (red).
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS438074

Flow Cytometry (FC) of anti-Human Nuclear Antigen antibody

Flow Cytometric Analysis of PFA-fixed Raji cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS438074

Flow Cytometry (FC) of anti-Human Nuclear Antigen antibody

Flow Cytometric analysis of Human Nuclear Antigen on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled HNA Monoclonal Antibody (235-1)
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS438074

Flow Cytometry (FC) of anti-Human Nuclear Antigen antibody

Flow Cytometric analysis of Human Nuclear Antigen on HeLa cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled HNA Monoclonal Antibody (235- 1).
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS438074

SDS-PAGE (SDS) of anti-Human Nuclear Antigen antibody

SDS-PAGE Analysis Purified Human-Nuclear-Antigen Mouse Monoclonal Antibody (235-1). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody, SDS-PAGE (SDS) – Cat# MBS438074

Flow Cytometry (FC) of anti-Human Nuclear Antigen antibody

Flow Cytometry of Human Nuclear Ag (HNA) on 293T cells. Black: Cells alone; Grey: Isotype Control; Green: CF488-labeled HNA MAb (235-1)
Mouse Anti-Human Nuclear Antigen Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS438074

Product References and Citations for anti-Human Nuclear Antigen antibody

1. Rosario Sanchez-Pernaute et. al. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease. Brain. 2008; 131(8): 2127-2139.

Other Notes

Small volumes of anti-Human Nuclear Antigen antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
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