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Cytokeratin 20 (KRT20) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS4381186

MBS4381186 is an antibody targeting KRT20. Also known as Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker). Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Cytokeratin 20 (KRT20), Monoclonal Antibody
Full Product Name
Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker)
Product Gene Name
Product Synonym Names
CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20; type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20
Clonality
Monoclonal
Isotype
IgG, kappa
Clone Number
KRT20/3145
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Purity/Purification
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Specificity
This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT20 is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
Preparation and Storage
Antibody with azide: store at 2 to 8 degree C.
Antibody without azide: store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
46kDa
OMIM
608218
3D Structure
ModBase 3D Structure for P35900
Positive Control
HT29 cells. Colon Carcinoma.
Immunogen
Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary)
Cellular Localization
Cytoplasmic
Hu-chromosome Location
17q21.2
Applications Tested/Suitable for anti-KRT20 antibody
Immunohistochemistry (IHC) Paraffin
Application Notes for anti-KRT20 antibody
Immunohistochemistry (Formalin-fixed) (0.1-0.2ug/ml for 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-KRT20 antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 20 Mouse Monoclonal Antibody (KRT20/3145).
Mouse Anti-KRT20 Monoclonal Antibody – Cat# MBS4381186

Immunohistochemistry (IHC) of anti-KRT20 antibody

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 20 Mouse Monoclonal Antibody (KRT20/3145).
Mouse Anti-KRT20 Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS4381186

SDS-Page of anti-KRT20 antibody

SDS-PAGE Analysis Purified CK20 Mouse Monoclonal Antibody (KRT20/3145). Confirmation of Purity and Integrity of Antibody.
Mouse Anti-KRT20 Monoclonal Antibody, SDS-Page – Cat# MBS4381186

Product References and Citations for anti-KRT20 antibody

1. Wauters, C.C., et al. 1995. Hum. Pathol. 26: 852-855. 2. Moll, R., et al. 1993. Differentiation 53: 75-93.

Other Notes

Small volumes of anti-KRT20 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for KRT20. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
keratin, type I cytoskeletal 20
NCBI Official Synonym Full Names
keratin 20
NCBI Official Symbol
KRT20
NCBI Official Synonym Symbols
K20; CD20; CK20; CK-20; KRT21
UniProt Protein Name
Keratin, type I cytoskeletal 20
UniProt Synonym Protein Names
Cytokeratin-20; CK-20; Keratin-20; K20; Protein IT
Protein Family
UniProt Gene Name
KRT20
UniProt Synonym Gene Names
CK-20; K20
UniProt Entry Name
K1C20_HUMAN
NCBI Protein Information
keratin, type I cytoskeletal 20

NCBI Summary for KRT20

The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq, Jul 2008]
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UniProt Comments for KRT20

K20: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; intermediate filament

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: regulation of protein secretion; apoptosis; cellular response to stress; intermediate filament organization
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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