Rat Anti-ITGAM Monoclonal Antibody – Cat# MBS438151

ITGAM Antibody – Rat Monoclonal

Name: ITGAM Antibody; Monoclonal; Rat; Human/Rabbit/Mouse-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS438151
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438151

MBS438151 is an antibody targeting ITGAM. Also known as CD11b. Reactive in Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit and Mouse.. Host: Rat. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CD11b (ITGAM), Monoclonal Antibody

Full Product Name

CD11b

Product Gene Name

anti-ITGAM antibody

Product Synonym Names

CD11 antigen-like family member B; CD11b/CD18; CD49d; Cell surface glycoprotein MAC-1 subunit alpha; Complement Component Receptor 3 Alpha; CR3 Alpha Chain (CR3A); Integrin alpha-M; Integrin beta 2 alpha subunit; ITGAM; Leukocyte adhesion receptor MO1; Ly-40; Mac-1a; MAC1; Mac1; alpha subunit; MAC1A; Macrophage antigen alpha polypeptide; MO1A; Neutrophil adherence receptor alpha M subunit

Clonality

Monoclonal

Isotype

IgG2b, kappa

Clone Number

M1/70

Host

Rat

Species Reactivity

Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit and Mouse.

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin åM combines with the Integrin ?2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin åM/?2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.

Preparation and Storage

Store at 2 to 8 degree C. Antibody is stable for 24 months.

Molecular Weight

95kDa; 170kDa

OMIM

gene 609939

3D Structure

ModBase 3D Structure for P11215

Localization

Cell surface

Positive Control

Monocytes & granulocytes. Lymph nodes and tonsils.

Human Chromosomal Location

16p11.2

Immunogen

B10 mouse spleen cells enriched for T lymphocytes

Unigene

172631 (Human)

Applications Tested/Suitable for anti-ITGAM antibody

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Functional Studies , Immunoprecipitation (IP), Immunohistology (IH)

Application Notes for anti-ITGAM antibody

ELISA (For coating, order Ab without BSA) Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Functional Studies (Order Ab without BSA & Azide); Immunoprecipitation (0.5-1ug/500ug protein lysate); Immunohistology (Frozen) (0.5-1.0ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined.

Product References and Citations for anti-ITGAM antibody

1. Springer T, et al. 1978. Eur. J. Immunol. 8:539.
2. Ault K and Springer T. 1981. J. Immunol. 126:359.
3. Springer TA, et al. 1982. Immunol. Rev. 68:171.
4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766.
5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112.

Other Notes

Small volumes of anti-ITGAM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ITGAM. It may not necessarily be applicable to this product.

NCBI GI #

88501734

NCBI GeneID

3684

NCBI Accession #

NP_000623.2

UniProt Primary Accession #

P11215

UniProt Secondary Accession #

Q4VAK0; Q4VAK1; Q4VAK2

UniProt Related Accession #

P11215

GenBank Nucleotide #

NM_000632.3

NCBI Official Full Name

integrin alpha-M isoform 2

NCBI Official Synonym Full Names

integrin, alpha M (complement component 3 receptor 3 subunit)

NCBI Official Symbol

ITGAM

NCBI Official Synonym Symbols

CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6

UniProt Protein Name

Integrin alpha-M

UniProt Synonym Protein Names

CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD_antigen: CD11b

Protein Family

Integrin

UniProt Gene Name

ITGAM

UniProt Synonym Gene Names

CD11B; CR3A

UniProt Entry Name

ITAM_HUMAN

NCBI Protein Information

integrin alpha-M; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subunit

NCBI Summary for ITGAM

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

UniProt Comments for ITGAM

ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes.

Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16p11.2

Cellular Component: cell surface; plasma membrane; nucleus; integrin complex; external side of plasma membrane

Molecular Function: heparan sulfate proteoglycan binding; heparin binding; opsonin binding; protein binding; protein heterodimerization activity; metal ion binding; glycoprotein binding

Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; extracellular matrix organization and biogenesis; microglia development; cellular extravasation; activated T cell proliferation; toll-like receptor signaling pathway; innate immune response; cell adhesion; blood coagulation; toll-like receptor 4 signaling pathway; leukocyte migration; leukocyte migration during inflammatory response

Disease: Systemic Lupus Erythematosus, Susceptibility To, 6

Research Articles on ITGAM

1. Although CD11b was more frequently expressed in blast cells of patients with intermediate and unfavorable cytogenetic risk groups, this feature did not translate into different clinical outcome.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ITGAM antibody

Reference Product	PubMed Publications
ITGB2 antibody	>1638 publications with ITGAM and ITGB2
ICAM1 antibody	>437 publications with ITGAM and ICAM1
ICAM2 antibody	>20 publications with ITGAM and ICAM2
PLAUR antibody	>14 publications with ITGAM and PLAUR
THY1 antibody	>14 publications with ITGAM and THY1
ICAM4 antibody	>6 publications with ITGAM and ICAM4
SELP antibody	>6 publications with ITGAM and SELP
JAM3 antibody	>5 publications with ITGAM and JAM3

Pathways associated with anti-ITGAM antibody

Products by Pathway	Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hematopoietic Cell Lineage Pathway antibodies	Hematopoietic Cell Lineage Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram

Diseases associated with anti-ITGAM antibody

Disease Name	Pubmed Publications
Inflammation Antibodies	>1371 publications with ITGAM and Inflammation
Necrosis Antibodies	>812 publications with ITGAM and Necrosis
Disease Models, Animal Antibodies	>798 publications with ITGAM and Disease Models, Animal
Nervous System Diseases Antibodies	>620 publications with ITGAM and Nervous System Diseases
Cardiovascular Diseases Antibodies	>493 publications with ITGAM and Cardiovascular Diseases
Brain Diseases Antibodies	>422 publications with ITGAM and Brain Diseases
Neoplasms, Experimental Antibodies	>176 publications with ITGAM and Neoplasms, Experimental
Heart Diseases Antibodies	>154 publications with ITGAM and Heart Diseases
Pain Antibodies	>124 publications with ITGAM and Pain
Atherosclerosis Antibodies	>121 publications with ITGAM and Atherosclerosis

Organs/Tissues associated with anti-ITGAM antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>4430 publications with ITGAM and Blood
Bone Antibodies	>823 publications with ITGAM and Bone
Bone Marrow Antibodies	>765 publications with ITGAM and Bone Marrow
Brain Antibodies	>610 publications with ITGAM and Brain
Lung Antibodies	>525 publications with ITGAM and Lung
Spleen Antibodies	>505 publications with ITGAM and Spleen
Lymph Node Antibodies	>302 publications with ITGAM and Lymph Node
Nerve Antibodies	>238 publications with ITGAM and Nerve
Intestine Antibodies	>154 publications with ITGAM and Intestine
Kidney Antibodies	>139 publications with ITGAM and Kidney