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ST6GAL1 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438268

MBS438268 is an antibody targeting ST6GAL1. Also known as CDw75 (B-Cell Marker) Mouse Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
CDw75 (ST6GAL1), Monoclonal Antibody
Full Product Name
CDw75 (B-Cell Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-ST6GAL1 antibody
Product Synonym Names
6-sialyltransferase 1 (6-ST1), Alpha 2,6 ST1, Beta galactoside alpha 2,6 sialyltransferase 1, CMP N acetylneuraminate beta galactosamide alpha 2,6 sialyltransferase 1, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2, SIAT1, ST6 beta galactosamide alpha 2,6 sialyltranferase 1, ST6Gal I, ST6N
Clonality
Monoclonal
Isotype
IgM, kappa
Clone Number
LN-1
Host
Mouse
Species Reactivity
Human
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by PEG precipitation. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Recognizes a neuraminidase-sensitive sialoprotein (CDw75), present on cell membrane and cytoplasm of germinal center B-cells and derived lymphomas. This MAb reacts with RBC precursors of bone marrow, ductal and ciliated epithelial cells of kidney, breast, prostate, pancreas, lung, and with glioblastomas, astrocytomas, and Reed Sternberg cells in lymphocyte predominant Hodgkin's disease. It is shown to be a helpful antibody for ascribing a B-cell phenotype in known lymphoid tissues.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
46kDa
OMIM
109675
3D Structure
ModBase 3D Structure for P15907
Cellular Localization
Cell Surface and Cytoplasmic
Immunogen
Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes.
Hu-Chromosome Location
3q27.3
Positive Control
HeLa or Daudi Cells. Germinal center B-cells in a lymph node or tonsil. Use LN1 s ability to stain surface of erythrocytes as an internal positive control.
Applications Tested/Suitable for anti-ST6GAL1 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-ST6GAL1 antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-ST6GAL1 antibody

Formalin-fixed, paraffin-embedded normal human Spleen stained with CDw75 Monoclonal Antibody (LN-1).
Mouse Anti-ST6GAL1 Monoclonal Antibody – Cat# MBS438268

Testing Data of anti-ST6GAL1 antibody

Surface staining of human lymphocytes with CDw75 Monoclonal Antibody (LN-1) (red) and isotype control (green). PPI negative population analyzed.
Mouse Anti-ST6GAL1 Monoclonal Antibody, Testing Data – Cat# MBS438268

Product References and Citations for anti-ST6GAL1 antibody

Epstein AL et. al. J of Immunology 133: 1028-1036, 1984. Marder RJ et. al. Lab Invest 52: 497-504, 1985.

Other Notes

Small volumes of anti-ST6GAL1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for ST6GAL1. It may not necessarily be applicable to this product.

NCBI GI #
4506949
NCBI GeneID
6480
NCBI Accession #
NP_003023.1
UniProt Primary Accession #
P15907
UniProt Secondary Accession #
A8KA14; B2R513; D3DNV3
UniProt Related Accession #
P15907
GenBank Nucleotide #
NM_003032.2
NCBI Official Full Name
beta-galactoside alpha-2,6-sialyltransferase 1 isoform a
NCBI Official Synonym Full Names
ST6 beta-galactoside alpha-2,6-sialyltransferase 1
NCBI Official Symbol
ST6GAL1
NCBI Official Synonym Symbols
ST6N; SIAT1; ST6GalI
UniProt Protein Name
Beta-galactoside alpha-2,6-sialyltransferase 1
UniProt Synonym Protein Names
B-cell antigen CD75; CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,6-sialyltransferase 1; ST6Gal I; ST6GalI; Sialyltransferase 1
Protein Family
Beta-galactoside alpha-2,6-sialyltransferase
UniProt Gene Name
ST6GAL1
UniProt Synonym Gene Names
SIAT1; Alpha 2,6-ST 1; ST6GalI
UniProt Entry Name
SIAT1_HUMAN
NCBI Protein Information
beta-galactoside alpha-2,6-sialyltransferase 1

NCBI Summary for ST6GAL1

This gene encodes a member of glycosyltransferase family 29. The encoded protein is a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. The protein, which is normally found in the Golgi but can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CD75, and CD76. This gene has been incorrectly referred to as CD75. Three transcript variants encoding two different isoforms have been described. [provided by RefSeq, Aug 2009]
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UniProt Comments for ST6GAL1

SIAT1: a type II membrane protein that catalyzes the transfer of sialic acid from CMP-sialic acid to galactose-containing substrates. Is normally found in the trans cisternae of the Golgi but which can be proteolytically processed to a soluble form, is involved in the generation of the cell-surface carbohydrate determinants and differentiation antigens HB-6, CDw75, and CD76. This protein is a member of glycosyltransferase family 29. Three transcript variants encoding two different isoforms have been found.

Protein type: Membrane protein, integral; Glycan Metabolism - N-glycan biosynthesis; Transferase; EC 2.4.99.1

Chromosomal Location of Human Ortholog: 3q27.3

Cellular Component: Golgi membrane

Molecular Function: beta-galactoside alpha-2,6-sialyltransferase activity; protein binding; sialyltransferase activity

Biological Process: humoral immune response; N-acetylneuraminate metabolic process; O-glycan processing; oligosaccharide metabolic process; protein amino acid N-linked glycosylation via asparagine
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Research Articles on ST6GAL1

1. These results suggest a role for ST6Gal-I to promote the growth and invasion of osteosarcoma cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-ST6GAL1 antibody

 Products by Pathway  Pathway Diagram
 Asparagine N-linked Glycosylation Pathway antibodies  Asparagine N-linked Glycosylation Pathway Diagram
 Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway antibodies  Biosynthesis Of The N-glycan Precursor (dolichol Lipid-linked Oligosaccharide, LLO) And Transfer To A Nascent Protein Pathway Diagram
 Metabolic Pathways antibodies  Metabolic Pathways Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 N-Glycan Antennae Elongation Pathway antibodies  N-Glycan Antennae Elongation Pathway Diagram
 N-Glycan Biosynthesis Pathway antibodies  N-Glycan Biosynthesis Pathway Diagram
 N-Glycan Biosynthesis Pathway antibodies  N-Glycan Biosynthesis Pathway Diagram
 N-glycan Antennae Elongation In The Medial/trans-Golgi Pathway antibodies  N-glycan Antennae Elongation In The Medial/trans-Golgi Pathway Diagram
 N-glycan Biosynthesis, Complex Type Pathway antibodies  N-glycan Biosynthesis, Complex Type Pathway Diagram
 N-glycan Biosynthesis, Complex Type Pathway antibodies  N-glycan Biosynthesis, Complex Type Pathway Diagram

Diseases associated with anti-ST6GAL1 antibody

 Disease Name  Pubmed Publications
 Neoplasm Metastasis Antibodies  >14 publications with ST6GAL1 and Neoplasm Metastasis
 Liver Diseases Antibodies  >11 publications with ST6GAL1 and Liver Diseases
 Liver Neoplasms Antibodies  >8 publications with ST6GAL1 and Liver Neoplasms
 Inflammation Antibodies  >8 publications with ST6GAL1 and Inflammation
 Cardiovascular Diseases Antibodies  >6 publications with ST6GAL1 and Cardiovascular Diseases
 Carcinoma, Hepatocellular Antibodies  >5 publications with ST6GAL1 and Carcinoma, Hepatocellular
 Kidney Diseases Antibodies  >4 publications with ST6GAL1 and Kidney Diseases
 Breast Neoplasms Antibodies  >4 publications with ST6GAL1 and Breast Neoplasms
 Disease Models, Animal Antibodies  >3 publications with ST6GAL1 and Disease Models, Animal
 Cell Transformation, Neoplastic Antibodies  >3 publications with ST6GAL1 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-ST6GAL1 antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >36 publications with ST6GAL1 and Blood
 Liver Antibodies  >18 publications with ST6GAL1 and Liver
 Lymph Antibodies  >10 publications with ST6GAL1 and Lymph
 Lymph Node Antibodies  >10 publications with ST6GAL1 and Lymph Node
 Lung Antibodies  >9 publications with ST6GAL1 and Lung
 Vascular Antibodies  >6 publications with ST6GAL1 and Vascular
 Ovary Antibodies  >6 publications with ST6GAL1 and Ovary
 Stomach Antibodies  >6 publications with ST6GAL1 and Stomach
 Brain Antibodies  >6 publications with ST6GAL1 and Brain
 Bone Antibodies  >5 publications with ST6GAL1 and Bone
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