Mouse Anti-MYOG Monoclonal Antibody – Cat# MBS438336

Formalin-fixed, paraffin-embedded Rhabdomyosarcoma stained with Myogenin Monoclonal Antibody (MGN185)

Myogenin (MYOG) Antibody – Mouse Monoclonal

Name: MYOG Antibody; Monoclonal; Mouse; Human/Mouse/Rat/Pig-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS438336
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438336

MBS438336 is an antibody targeting MYOG. Also known as Myogenin (Skeletal Muscle Marker) Mouse Monoclonal Antibody. Reactive in Human, Mouse, Rat, Cat, Pig. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Myogenin, Monoclonal Antibody

Full Product Name

Myogenin (Skeletal Muscle Marker) Mouse Monoclonal Antibody

Product Gene Name

anti-MYOG antibody

Product Synonym Names

bHLHc3, cb553, Class C basic helix-loop-helix protein 3, Myf-4, MYF4, MYOG, Myogenic factor 4

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

MGN185

Host

Mouse

Species Reactivity

Human, Mouse, Rat, Cat, Pig

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Specificity

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

34kDa

OMIM

159980

3D Structure

ModBase 3D Structure for P15173

Cellular Localization

Nuclear

Immunogen

Human myogenin recombinant protein

Hu-Chromosome Location

1q32.1

Positive Control

Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.

Applications Tested/Suitable for anti-MYOG antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

Application Notes for anti-MYOG antibody

Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blot (0.5-1ug/ml)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-MYOG antibody

Wang NP et. al. Am J Pathol 1995, 147:1799-1810

Other Notes

Small volumes of anti-MYOG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for MYOG. It may not necessarily be applicable to this product.

NCBI GI #

18765727

NCBI GeneID

4656

NCBI Accession #

NP_002470.2

UniProt Primary Accession #

P15173

UniProt Secondary Accession #

Q53XW6

UniProt Related Accession #

P15173

GenBank Nucleotide #

NM_002479.5

NCBI Official Full Name

myogenin

NCBI Official Synonym Full Names

myogenin (myogenic factor 4)

NCBI Official Symbol

MYOG

NCBI Official Synonym Symbols

MYF4; myf-4; bHLHc3

UniProt Protein Name

Myogenin

UniProt Synonym Protein Names

Class C basic helix-loop-helix protein 3; bHLHc3; Myogenic factor 4; Myf-4

Protein Family

Myogenin

UniProt Gene Name

MYOG

UniProt Synonym Gene Names

BHLHC3; MYF4; bHLHc3; Myf-4

UniProt Entry Name

MYOG_HUMAN

NCBI Protein Information

myogenin

NCBI Summary for MYOG

Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq, Jul 2008]

UniProt Comments for MYOG

myogenin: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 1q31-q41

Cellular Component: nucleoplasm; nucleus; transcription factor complex

Molecular Function: chromatin DNA binding; protein binding; protein heterodimerization activity; RNA polymerase II transcription factor activity, enhancer binding; sequence-specific DNA binding; transcription factor activity

Biological Process: mRNA transcription from RNA polymerase II promoter; muscle cell fate commitment; negative regulation of cell proliferation; positive regulation of muscle atrophy; positive regulation of muscle cell differentiation; positive regulation of myoblast differentiation; positive regulation of skeletal muscle fiber development; positive regulation of transcription from RNA polymerase II promoter; regulation of satellite cell proliferation; response to denervation involved in regulation of muscle adaptation; response to electrical stimulus involved in regulation of muscle adaptation; response to muscle activity involved in regulation of muscle adaptation; skeletal muscle development; striated muscle atrophy

Research Articles on MYOG

1. results indicate that myogenin is a positive regulator in transcriptional regulation of MEGF10 in skeletal muscle

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-MYOG antibody

Reference Product	PubMed Publications
PAX7 antibody	>61 publications with MYOG and PAX7
MEF2C antibody	>21 publications with MYOG and MEF2C
MEF2A antibody	>17 publications with MYOG and MEF2A
CTNNB1 antibody	>11 publications with MYOG and CTNNB1
SRF antibody	>9 publications with MYOG and SRF
MEF2D antibody	>4 publications with MYOG and MEF2D
FGF4 antibody	>3 publications with MYOG and FGF4
TCF3 antibody	>2 publications with MYOG and TCF3

Pathways associated with anti-MYOG antibody

Products by Pathway	Pathway Diagram
CDO In Myogenesis Pathway antibodies	CDO In Myogenesis Pathway Diagram
Developmental Biology Pathway antibodies	Developmental Biology Pathway Diagram
Hypertrophy Model Pathway antibodies	Hypertrophy Model Pathway Diagram
Id Signaling Pathway antibodies	Id Signaling Pathway Diagram
Myogenesis Pathway antibodies	Myogenesis Pathway Diagram
Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway antibodies	Regulation Of Wnt-mediated Beta Catenin Signaling And Target Gene Transcription Pathway Diagram

Diseases associated with anti-MYOG antibody

Disease Name	Pubmed Publications
Atrophy Antibodies	>36 publications with MYOG and Atrophy
Hypertrophy Antibodies	>33 publications with MYOG and Hypertrophy
Necrosis Antibodies	>22 publications with MYOG and Necrosis
Inflammation Antibodies	>17 publications with MYOG and Inflammation
Fibrosis Antibodies	>17 publications with MYOG and Fibrosis
Insulin Resistance Antibodies	>11 publications with MYOG and Insulin Resistance
Hyperplasia Antibodies	>11 publications with MYOG and Hyperplasia
Cell Transformation, Neoplastic Antibodies	>9 publications with MYOG and Cell Transformation, Neoplastic
Chromosome Aberrations Antibodies	>7 publications with MYOG and Chromosome Aberrations
Kidney Diseases Antibodies	>7 publications with MYOG and Kidney Diseases

Organs/Tissues associated with anti-MYOG antibody

Organ/Tissue Name	Pubmed Publications
Muscle Antibodies	>849 publications with MYOG and Muscle
Nerve Antibodies	>56 publications with MYOG and Nerve
Eye Antibodies	>9 publications with MYOG and Eye
Placenta Antibodies	>1 publications with MYOG and Placenta