Mouse Anti-GLG1 Monoclonal Antibody – Cat# MBS438610

Formalin-fixed, paraffin-embedded human Pancreas stained with GLG1 Monoclonal Antibody (GLG1/970).

GLG1 Antibody – Mouse Monoclonal

Name: GLG1 Antibody; Monoclonal; Mouse; Human/Mouse/Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS438610
Price: 280 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438610

MBS438610 is an antibody targeting GLG1. Also known as GLG1 (Golgi Glycoprotein 1) (Marker for Human Cells) Mouse Monoclonal Antibody. Reactive in Human.Does not react with Mouse and Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

GLG1 (Golgi Glycoprotein 1), Monoclonal Antibody

Full Product Name

GLG1 (Golgi Glycoprotein 1) (Marker for Human Cells) Mouse Monoclonal Antibody

Product Gene Name

anti-GLG1 antibody

Product Synonym Names

CFR-1; Cysteine-rich fibroblast growth factor receptor; E-selectin ligand-1 (ESL-1); Golgi Glycoprotein 1 (GLG1); Golgi apparatus protein 1; Golgi sialoglycoprotein MG-160; Slectin, endothelial cell, ligand

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone Number

GLG1/970

Host

Mouse

Species Reactivity

Human.
Does not react with Mouse and Rat

Form/Format

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Specificity

This MAb recognizes a protein of 134kDa, which binds fibroblast growth factor and E-selectin (cell-adhesion lectin on endothelial cells mediating the binding of neutrophils). Fucosylation is essential for binding to E-selectin. It contains sialic acid residues and 16 Cys-rich GLG1 repeats. This MAb can be used to stain the Golgi complex in cell or tissue preparations and can be used as a Golgi marker in subcellular fractions. It produces a diffuse staining pattern of the Golgi zone in normal and malignant cells. This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. The Golgi apparatus is an organelle present in all eukaryotic cells that forms a part of the endomembrane system. The primary function of the Golgi apparatus is to process and package macromolecules synthesized by the cell for exocytosis or use within the cell. The Golgi is made up of a stack of flattened, membrane-bound sacs known as cisternae, with three functional regions: the cis face, medial region and trans face. Each region consists of various enzymes that selectively modify the macromolecules passing though them, depending on where they are destined to reside. Several spherical vesicles that have budded off of the Golgi are present surrounding the main cisternae. The Golgi tends to be more pronounced and numerous in cells that make and secrete many substances such as plasma B cells.

Preparation and Storage

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Molecular Weight

134kDa

OMIM

600753

3D Structure

ModBase 3D Structure for Q92896

Cellular Localization

Golgi complex in Cytoplasm

Immunogen

Golgi fraction from human liver cells

Hu-Chromosome Location

16q22.3

Positive Control

HepG2, A431 or HeLa cells. Placenta, Tonsil, Testis and Ovary.

Applications Tested/Suitable for anti-GLG1 antibody

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin

Application Notes for anti-GLG1 antibody

Flow Cytometry (0.5-1.0ug/million cells in 0.1ml)
Immunofluorescence (0.5-1ug/ml)
Western Blot (0.5-1.0ug/ml)
Immunocytochemistry (Acetone or paraformaldehyde fixed) (0.5-1ug/ml for 30 min)
Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Product References and Citations for anti-GLG1 antibody

Nakamura et. al. 1995. J. Cell Biol. 131:1715-26. Nakamura et.al. 1997. Cell. 89(3):445-55

Other Notes

Small volumes of anti-GLG1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for GLG1. It may not necessarily be applicable to this product.

NCBI GI #

224586815

NCBI GeneID

2734

NCBI Accession #

NP_001139138.1

UniProt Primary Accession #

Q92896

UniProt Secondary Accession #

Q13221; Q6P9D1; B7Z8Y4; D3DUJ7

UniProt Related Accession #

Q92896

GenBank Nucleotide #

NM_001145666.1

NCBI Official Full Name

Golgi apparatus protein 1 isoform 2

NCBI Official Synonym Full Names

golgi glycoprotein 1

NCBI Official Symbol

GLG1

NCBI Official Synonym Symbols

CFR-1; ESL-1; MG160; MG-160

UniProt Protein Name

Golgi apparatus protein 1

UniProt Synonym Protein Names

CFR-1; Cysteine-rich fibroblast growth factor receptor; E-selectin ligand 1; ESL-1; Golgi sialoglycoprotein MG-160

Protein Family

Glucose-1-phosphate adenylyltransferase

UniProt Gene Name

GLG1

UniProt Synonym Gene Names

CFR1; ESL1; MG160; ESL-1

UniProt Entry Name

GSLG1_HUMAN

NCBI Protein Information

Golgi apparatus protein 1

UniProt Comments for GLG1

GLG1: Binds fibroblast growth factor and E-selectin (cell- adhesion lectin on endothelial cells mediating the binding of neutrophils). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Vesicle

Chromosomal Location of Human Ortholog: 16q22.3

Cellular Component: Golgi apparatus; membrane; plasma membrane

Molecular Function: fibroblast growth factor binding; receptor binding

Biological Process: leukocyte migration

Research Articles on GLG1

1. Our results highlight the fact that interaction between APN and ESL-1 could provide a fundamental mechanism underlying the anti-atherogenic properties of APN.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-GLG1 antibody

Products by Pathway	Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Adhesion Molecules (CAMs) Pathway antibodies	Cell Adhesion Molecules (CAMs) Pathway Diagram
Cell Surface Interactions At The Vascular Wall Pathway antibodies	Cell Surface Interactions At The Vascular Wall Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram
TNF-alpha/NF-kB Signaling Pathway antibodies	TNF-alpha/NF-kB Signaling Pathway Diagram

Diseases associated with anti-GLG1 antibody

Disease Name	Pubmed Publications
Breast Neoplasms Antibodies	>2 publications with GLG1 and Breast Neoplasms
Brain Diseases Antibodies	>1 publications with GLG1 and Brain Diseases
Disease Models, Animal Antibodies	>1 publications with GLG1 and Disease Models, Animal
Prostatic Neoplasms Antibodies	>1 publications with GLG1 and Prostatic Neoplasms
Nervous System Diseases Antibodies	>1 publications with GLG1 and Nervous System Diseases
Hyperplasia Antibodies	>1 publications with GLG1 and Hyperplasia

Organs/Tissues associated with anti-GLG1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>3 publications with GLG1 and Blood
Vascular Antibodies	>2 publications with GLG1 and Vascular
Brain Antibodies	>2 publications with GLG1 and Brain
Lymph Node Antibodies	>1 publications with GLG1 and Lymph Node
Stomach Antibodies	>1 publications with GLG1 and Stomach
Pancreas Antibodies	>1 publications with GLG1 and Pancreas
Prostate Antibodies	>1 publications with GLG1 and Prostate
Kidney Antibodies	>1 publications with GLG1 and Kidney
Embryonic Tissue Antibodies	>1 publications with GLG1 and Embryonic Tissue
Lymph Antibodies	>1 publications with GLG1 and Lymph