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Vimentin (VIM) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS438855

MBS438855 is an antibody targeting VIM. Also known as Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody. Reactive in Human.Does not react with Mouse and Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Vimentin, Monoclonal Antibody
Full Product Name
Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-VIM antibody
Product Synonym Names
VIM
Clonality
Monoclonal
Isotype
IgG1
Clone Number
VM1170
Host
Mouse
Species Reactivity
Human.
Does not react with Mouse and Rat
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
57-60kDa
OMIM
116300
3D Structure
ModBase 3D Structure for P08670
Cellular Localization
Cytoplasmic
Immunogen
Recombinant full-length human vimentin protein
Hu-Chromosome Location
10p12.33
Positive Control
Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.
Applications Tested/Suitable for anti-VIM antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
Application Notes for anti-VIM antibody
Flow Cytometry (0.5-1ug/million cells in 0.1ml)
Immunofluorescence (1-2ug/ml)
Western Blot (0.25-0.5ug/ml)
Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

Immunohistochemistry (IHC) of anti-VIM antibody

Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Monoclonal Antibody (VM1170).
Mouse Anti-VIM Monoclonal Antibody – Cat# MBS438855

Immunohistochemistry (IHC) of anti-VIM antibody

Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Vimentin Monoclonal Antibody (VM1170).
Mouse Anti-VIM Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438855

Immunohistochemistry (IHC) of anti-VIM antibody

Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with Vimentin Monoclonal Antibody (VM1170).
Mouse Anti-VIM Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438855

Immunohistochemistry (IHC) of anti-VIM antibody

Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).
Mouse Anti-VIM Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438855

Immunohistochemistry (IHC) of anti-VIM antibody

Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).
Mouse Anti-VIM Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438855

Immunohistochemistry (IHC) of anti-VIM antibody

Formalin-fixed, paraffin-embedded human Uterus stained with Vimentin Monoclonal Antibody (VM1170).
Mouse Anti-VIM Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS438855

Product References and Citations for anti-VIM antibody

Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143.

Other Notes

Small volumes of anti-VIM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for VIM. It may not necessarily be applicable to this product.

NCBI GI #
62414289
NCBI GeneID
7431
NCBI Accession #
NP_003371.2
UniProt Primary Accession #
P08670
UniProt Secondary Accession #
Q15867; Q15868; Q15869; Q548L2; Q6LER9; Q8N850; Q96ML2; Q9NTM3; B0YJC2; D3DRU4
UniProt Related Accession #
P08670
GenBank Nucleotide #
NM_003380.3
NCBI Official Full Name
vimentin
NCBI Official Synonym Full Names
vimentin
NCBI Official Symbol
VIM
NCBI Official Synonym Symbols
HEL113; CTRCT30
UniProt Protein Name
Vimentin
Protein Family
Vimentin
UniProt Gene Name
VIM
UniProt Entry Name
VIME_HUMAN
NCBI Protein Information
vimentin

NCBI Summary for VIM

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]
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UniProt Comments for VIM

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

Research Articles on VIM

1. Studied the link between VEGFR-1 and PKG-1 in copper regression of cardiomyocyte hypertrophy.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-VIM antibody

 Reference Product  PubMed Publications
 CASP3 antibody  >8 publications with VIM and CASP3
 AKT1 antibody  >4 publications with VIM and AKT1
 TPM3 antibody  >1 publications with VIM and TPM3
 CASP8 antibody  >1 publications with VIM and CASP8

Pathways associated with anti-VIM antibody

 Products by Pathway  Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Apoptosis Pathway antibodies  Apoptosis Pathway Diagram
 Apoptotic Cleavage Of Cellular Proteins Pathway antibodies  Apoptotic Cleavage Of Cellular Proteins Pathway Diagram
 Apoptotic Execution Phase Pathway antibodies  Apoptotic Execution Phase Pathway Diagram
 Aurora B Signaling Pathway antibodies  Aurora B Signaling Pathway Diagram
 Caspase Cascade In Apoptosis Pathway antibodies  Caspase Cascade In Apoptosis Pathway Diagram
 Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway antibodies  Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 Epstein-Barr Virus Infection Pathway antibodies  Epstein-Barr Virus Infection Pathway Diagram
 MicroRNAs In Cancer Pathway antibodies  MicroRNAs In Cancer Pathway Diagram

Diseases associated with anti-VIM antibody

 Disease Name  Pubmed Publications
 Neurodegenerative Diseases Antibodies  >185 publications with VIM and Neurodegenerative Diseases
 Inflammation Antibodies  >70 publications with VIM and Inflammation
 Kidney Diseases Antibodies  >46 publications with VIM and Kidney Diseases
 Disease Models, Animal Antibodies  >45 publications with VIM and Disease Models, Animal
 Neoplasm Metastasis Antibodies  >43 publications with VIM and Neoplasm Metastasis
 Breast Neoplasms Antibodies  >42 publications with VIM and Breast Neoplasms
 Liver Diseases Antibodies  >39 publications with VIM and Liver Diseases
 Fibrosis Antibodies  >38 publications with VIM and Fibrosis
 Neoplasm Invasiveness Antibodies  >28 publications with VIM and Neoplasm Invasiveness
 Necrosis Antibodies  >28 publications with VIM and Necrosis

Organs/Tissues associated with anti-VIM antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >550 publications with VIM and Brain
 Blood Antibodies  >250 publications with VIM and Blood
 Muscle Antibodies  >133 publications with VIM and Muscle
 Bone Antibodies  >89 publications with VIM and Bone
 Nerve Antibodies  >89 publications with VIM and Nerve
 Kidney Antibodies  >69 publications with VIM and Kidney
 Liver Antibodies  >63 publications with VIM and Liver
 Bone Marrow Antibodies  >55 publications with VIM and Bone Marrow
 Eye Antibodies  >53 publications with VIM and Eye
 Lung Antibodies  >51 publications with VIM and Lung
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