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CD3E Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439364

MBS439364 is an antibody targeting CD3E. Also known as CD3 (T-Cell Marker) Mouse Monoclonal Antibody. Reactive in Human, Mouse, Rat. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD3 (CD3E), Monoclonal Antibody
Full Product Name
CD3 (T-Cell Marker) Mouse Monoclonal Antibody
Product Gene Name
anti-CD3E antibody
Product Synonym Names
CD3E, CD3 epsilon, CD3 TCR complex, CD3E, CD3e antigen epsilon polypeptide (TiT3 complex), T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex
Clonality
Monoclonal
Isotype
IgG3, kappa
Clone Number
RIV9
Host
Mouse
Species Reactivity
Human, Mouse, Rat
Form/Format
200ug/ml of Ab Purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.
Specificity
Reacts with five invariable CD3 chains (designated as and) with molecular weight ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb is particularly useful for induction of T cell activation in vitro and in vivo due to its unusual IgG3 isotype.
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Non-hazardous. No MSDS required.
Molecular Weight
20kDa
OMIM
186830
3D Structure
ModBase 3D Structure for P07766
Cellular Localization
Cell Surface and Cytoplasmic
Immunogen
Human peripheral lymphocytes
Hu-Chromosome Location
11q23.3
Positive Control
Jurkat cells. Tonsil and lymph node
Applications Tested/Suitable for anti-CD3E antibody
Functional Studies, Flow Cytometry (FC), Immunofluorescence (IF)
Application Notes for anti-CD3E antibody
Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
Optimal dilution for a specific application should be determined

SDS-PAGE of anti-CD3E antibody

SDS-PAGE Analysis
CD3e Mouse Monoclonal Antibody (RIV9).
Confirmation of Integrity and Purity of Antibody
Mouse Anti-CD3E Monoclonal Antibody – Cat# MBS439364

Flow Cytometry (FC) of anti-CD3E antibody

Flow Cytometric Analysis of Jurkat Cells using CD3e Mouse Monoclonal Antibody (RIV9).
Followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)
Mouse Anti-CD3E Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS439364

Flow Cytometry (FC) of anti-CD3E antibody

Flow cytometry analysis of lymphocyte-gated PBMCs unstained (gray) or stained with CF488A-labeled CD3 mouse monoclonal antibody (RIV9) (green)
Mouse Anti-CD3E Monoclonal Antibody, Flow Cytometry (FC) – Cat# MBS439364

Immunofluorescence (IF) of anti-CD3E antibody

Immunofluorescence Analysis of Jurkat cells labeling CD3e with CD3e Mouse Monoclonal Antibody (RIV9) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red dot (Red).
Mouse Anti-CD3E Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS439364

Immunofluorescence (IF) of anti-CD3E antibody

Immunofluorescence Analysis of methanol-fixed human tonsil cryosection stained with CF488A CD3e clone RIV9 (green), mounted in EverBrite’ Mounting Medium with DAPI (nuclei, blue).
Mouse Anti-CD3E Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS439364

Product References and Citations for anti-CD3E antibody

Leerling MF et al. Quality control of anti human CD3 and CD4 monoclonal antibodies. Dev Biol Stand 1990, 71:191-200 Vaessen LM et al. RIV-9: a mouse IgG3 anti-human CD3 monoclonal antibody with strong antigen modulating and T cell eliminating properties. Transplant Proc 1989, 21:1026-7 Jiskoot W et al. Purification and stabilisation of a poorly soluble mouse IgG3 monoclonal antibody. J Immunol Methods 1991, 138(2):181-189 Ceuppens JL and Van Vaeck F. Human T cell activation induced by a monoclonal mouse IgG3 anti-CD3 antibody (RIV9) requires binding of the Fc part of the antibody to the monocytic 72-kDa high-affinity Fc receptor (FcRI). Cell Immunol 1989, 118(1):136-46 Knapp W et al. (eds) Leukocyte Typing IV, p245 and 1059, Oxford University Press, Oxford, 1989 Schlossman S et al. (eds) Leukocyte Typing V. Oxford University Press, Oxford, 1995. Alberola-Ila J et al. Stimulation through the TCR/CD3 complex up-regulates the CD2 surface expression on human T lymphocytes. J Immunol 1991, 146(4):108

Other Notes

Small volumes of anti-CD3E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD3E. It may not necessarily be applicable to this product.

NCBI GI #
4502671
NCBI GeneID
916
NCBI Accession #
NP_000724.1
UniProt Primary Accession #
P07766
UniProt Secondary Accession #
A8K997
UniProt Related Accession #
P07766
GenBank Nucleotide #
NM_000733.3
NCBI Official Full Name
T-cell surface glycoprotein CD3 epsilon chain
NCBI Official Synonym Full Names
CD3e molecule
NCBI Official Symbol
CD3E
NCBI Official Synonym Symbols
T3E; TCRE; IMD18
UniProt Protein Name
T-cell surface glycoprotein CD3 epsilon chain
UniProt Synonym Protein Names
T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e
Protein Family
T-cell surface glycoprotein
UniProt Gene Name
CD3E
UniProt Synonym Gene Names
T3E
UniProt Entry Name
CD3E_HUMAN
NCBI Protein Information
T-cell surface glycoprotein CD3 epsilon chain

NCBI Summary for CD3E

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]
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UniProt Comments for CD3E

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain.

Protein type: Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 11q23

Cellular Component: external side of plasma membrane; plasma membrane; T cell receptor complex

Molecular Function: protein binding; protein heterodimerization activity; receptor signaling protein activity; SH3 domain binding

Biological Process: G-protein coupled receptor protein signaling pathway; positive regulation of T cell proliferation; regulation of immune response; signal complex assembly; T cell costimulation; T cell receptor signaling pathway; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Immunodeficiency 18
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Research Articles on CD3E

1. The docking site for CD3 subunits on the T Cell receptor beta chain has been identified by solution NMR.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-CD3E antibody

 Reference Product  PubMed Publications
 LCK antibody  >20 publications with CD3E and LCK
 ZAP70 antibody  >20 publications with CD3E and ZAP70
 CD3D antibody  >15 publications with CD3E and CD3D
 CD3G antibody  >9 publications with CD3E and CD3G
 LAT antibody  >7 publications with CD3E and LAT
 SYK antibody  >4 publications with CD3E and SYK
 CD247 antibody  >3 publications with CD3E and CD247
 CSK antibody  >2 publications with CD3E and CSK

Pathways associated with anti-CD3E antibody

 Products by Pathway  Pathway Diagram
 Adaptive Immune System Pathway antibodies  Adaptive Immune System Pathway Diagram
 CXCR4-mediated Signaling Events Pathway antibodies  CXCR4-mediated Signaling Events Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Costimulation By The CD28 Family Pathway antibodies  Costimulation By The CD28 Family Pathway Diagram
 Downstream TCR Signaling Pathway antibodies  Downstream TCR Signaling Pathway Diagram
 Downstream Signaling In Naive CD8+ T Cells Pathway antibodies  Downstream Signaling In Naive CD8+ T Cells Pathway Diagram
 Generation Of Second Messenger Molecules Pathway antibodies  Generation Of Second Messenger Molecules Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram
 HTLV-I Infection Pathway antibodies  HTLV-I Infection Pathway Diagram

Diseases associated with anti-CD3E antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >23 publications with CD3E and Inflammation
 Disease Models, Animal Antibodies  >19 publications with CD3E and Disease Models, Animal
 Necrosis Antibodies  >14 publications with CD3E and Necrosis
 Immunologic Deficiency Syndromes Antibodies  >14 publications with CD3E and Immunologic Deficiency Syndromes
 Carcinoma Antibodies  >7 publications with CD3E and Carcinoma
 Lung Diseases Antibodies  >6 publications with CD3E and Lung Diseases
 Vascular Diseases Antibodies  >6 publications with CD3E and Vascular Diseases
 Severe Combined Immunodeficiency Antibodies  >5 publications with CD3E and Severe Combined Immunodeficiency
 Arthritis, Rheumatoid Antibodies  >5 publications with CD3E and Arthritis, Rheumatoid
 Neoplasm Metastasis Antibodies  >4 publications with CD3E and Neoplasm Metastasis

Organs/Tissues associated with anti-CD3E antibody

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >209 publications with CD3E and Blood
 Thymus Antibodies  >48 publications with CD3E and Thymus
 Spleen Antibodies  >37 publications with CD3E and Spleen
 Lymph Node Antibodies  >17 publications with CD3E and Lymph Node
 Liver Antibodies  >13 publications with CD3E and Liver
 Intestine Antibodies  >11 publications with CD3E and Intestine
 Lung Antibodies  >7 publications with CD3E and Lung
 Kidney Antibodies  >7 publications with CD3E and Kidney
 Brain Antibodies  >6 publications with CD3E and Brain
 Heart Antibodies  >5 publications with CD3E and Heart
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