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Filaggrin (FLG) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439956

MBS439956 is an antibody targeting FLG. Also known as Filaggrin (Keratinocyte Differentiation Marker). Reactive in Human. Others not tested.. Host: Mouse. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Filaggrin (FLG), Monoclonal Antibody
Full Product Name
Filaggrin (Keratinocyte Differentiation Marker)
Product Gene Name
Product Synonym Names
ATOD2; Epidermal Filaggrin; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin
Clonality
Monoclonal
Isotype
IgG1, kappa
Clone Number
FLG/1562
Host
Mouse
Species Reactivity
Human. Others not tested.
Form/Format
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
Filaggrin is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Active filaggrin is present at a level of the epidermis where keratinocytes are in transition between the live nucleated granular layer and the anucleate cornified layer, suggesting that filaggrin aids in the terminal differentiation process by facilitating apoptotic machinery.
Preparation and Storage
Antibody with azide - store at 2 to 8 degree C.
Antibody without azide - store at -20 to -80 degree C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
26-45kDa (Processed); 350kDa (Profilaggrin)
3D Structure
ModBase 3D Structure for P20930
Immunogen
Recombinant human Filaggrin protein fragment (aa 998-1104)
Cellular Localization
Cytoplasmic
Hu-chromosome Location
1q21.3
Positive Control
U-251-MG cells or Skin
Applications Tested/Suitable for anti-FLG antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin
Application Notes for anti-FLG antibody
FC/FACS: 0.5-1ug/million cells
IF: 1-2ug/ml
IHC-F: 0.5-1ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.

Immunohistochemistry (IHC) of anti-FLG antibody

Formalin-fixed, paraffin-embedded human Skin stained with Filaggrin Monoclonal Antibody (FLG/1562).
Mouse Anti-FLG Monoclonal Antibody – Cat# MBS439956

Immunohistochemistry (IHC) of anti-FLG antibody

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with TAG-72 Monoclonal Antibody (CC49).
Mouse Anti-FLG Monoclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS439956

Product References and Citations for anti-FLG antibody

Gan, S.Q., et al. 1990. Organization, structure, and polymorphisms of the human profilaggrin gene. Biochemistry 29: 9432-9440.

Other Notes

Small volumes of anti-FLG antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for FLG. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
filaggrin
NCBI Official Synonym Full Names
filaggrin
NCBI Official Symbol
FLG
NCBI Official Synonym Symbols
ATOD2
UniProt Protein Name
Filaggrin
Protein Family
UniProt Gene Name
FLG
NCBI Protein Information
filaggrin

NCBI Summary for FLG

The protein encoded by this gene is an intermediate filament-associated protein that aggregates keratin intermediate filaments in mammalian epidermis. It is initially synthesized as a polyprotein precursor, profilaggrin (consisting of multiple filaggrin units of 324 aa each), which is localized in keratohyalin granules, and is subsequently proteolytically processed into individual functional filaggrin molecules. Mutations in this gene are associated with ichthyosis vulgaris.[provided by RefSeq, Dec 2009]
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UniProt Comments for FLG

Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-FLG antibody

 Products by Pathway  Pathway Diagram
 AhR Pathway antibodies  AhR Pathway Diagram
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