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CD15 Antibody – Rabbit Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS439984

MBS439984 is an antibody targeting CD15. Also known as CD15/FUT4 (Reed-Sternberg Cell Marker). Reactive in Human, Mouse, Rat. Others not known.. Host: Rabbit. More details

$280 / 0.02 mg (With BSA & Azide at 0.2mg/mL) +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
CD15/FUT4, Monoclonal Antibody
Full Product Name
CD15/FUT4 (Reed-Sternberg Cell Marker)
Product Gene Name
Product Synonym Gene Name
FUT4
Product Synonym Names
3 Fucosyl N Acetyl Lactosamine; Alpha (1, 3) Fucosyltransferase; Alpha 13 fucosyltransferase FucT; ELAM Ligand Fucosyltransferase; ELFT; FCT3A; Fuc-TIV; Fucosyltransferase 4 Alpha 1 3 Fucosyltransferase Myeloid Specific; Fucosyltransferase 4; Galactoside 3 L Fucosyltransferase; Lewis X; LeX; SSEA1; Stage Specific Embryonic Antigen 1
Clonality
Monoclonal
Isotype
IgG
Clone Number
FUT4/1478R
Host
Rabbit
Species Reactivity
Human, Mouse, Rat. Others not known.
Form/Format
200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Specificity
CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkins disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.
Preparation and Storage
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20°C to -80°C.
Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Molecular Weight
~220kDa
3D Structure
ModBase 3D Structure for P22083
Human Unigene
654379
Human Gene Symbol
FUT4
Human Chromosome Location
11q21
Immunogen
Recombinant human full-length CD15 protein
Cellular Localization
Cell Surface and granular paranuclear
Positive Control
U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma
Applications Tested/Suitable for anti-CD15 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) (Formalin-fixed)
Application Notes for anti-CD15 antibody
Flow Cytometry (1-2ug/million cells);
Immunofluorescence (1-2ug/ml);
Immunohistochemistry (Formalin-fixed) (5-10ug/ml for 30 minutes at RT)
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 minutes followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC) of anti-CD15 antibody

Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R).
Rabbit Anti-CD15 Monoclonal Antibody – Cat# MBS439984

Immunofluorescence (IF) of anti-CD15 antibody

Immunofluorescence staining of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.
Rabbit Anti-CD15 Monoclonal Antibody, Immunofluorescence (IF) – Cat# MBS439984

Flow Cytometry (FC/FACS) of anti-CD15 antibody

Flow Cytometric Analysis of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).
Rabbit Anti-CD15 Monoclonal Antibody, Flow Cytometry (FC/FACS) – Cat# MBS439984

Product References and Citations for anti-CD15 antibody

Hakomori, S. 1992. LeX and related structures as adhesion molecules. Histochem. J. 24: 771-776.

Other Notes

Small volumes of anti-CD15 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CD15. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
alpha-(1,3)-fucosyltransferase 4
NCBI Official Synonym Full Names
fucosyltransferase 4
NCBI Official Symbol
FUT4
NCBI Official Synonym Symbols
LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV
UniProt Protein Name
Alpha-(1,3)-fucosyltransferase 4
UniProt Synonym Protein Names
ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase
UniProt Gene Name
FUT4
UniProt Synonym Gene Names
ELFT; FCT3A; Fuc-TIV; FucT-IV
NCBI Protein Information
alpha-(1,3)-fucosyltransferase 4

NCBI Summary for CD15

The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]

UniProt Comments for CD15

May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.CautionIt is uncertain whether Met-1 or Met-126 is the initiator.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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