Smac/Diablo Antibody – Rabbit Polyclonal

Second mitochondrial activator of caspases or Smac/DIABLO can promote apoptosis by relieving the inhibition of caspases by the IAPs (1&2). The other mitochondrial activator of apoptosis is cytochrome c (1). Smac/DIABLO export from the mitochondria into the cytosol can be triggered by cytotoxic drugs, DNA damage, and the ligation of FAS (CD95) death receptor (3). Once in the cytosol Smac/DIABLO eliminates the inhibitory effects of the IAPs through and amino terminal interaction with the IAP proteins, and thus allow the activation of caspases (2&4). The interaction of Smac/DIABLO's amino terminus with the IAP proteins has been localized to their BIR domains, for XIAP the key domain is BIR3 (4&5).

1.) Kim JS, Eom JI, Cheong JW, Choi AJ, Lee JK, Yang WI, Min YH. 2007. Protein kinase CK2alpha as an unfavorable prognostic marker and novel therapeutic target in acute myeloid leukemia. Clin Cancer Res. Feb 1;13(3):1019-28.

1. Du C., Fang M., Li Y., Li L., Wang X. 2000. Smac, a Mitochondrial Protein that promotes Cytochrome c-Dependent Caspase Activation by Eliminating IAP Inhibition. Cell 102:33-42.2. Springs S.L., Diavolitsis V.M., Goodhouse J., Mclendon G.L. 2002. The kinetics of translocation of Smac/DIABLO from the mitochondria to the cytosol in HeLa cells. J Biol Chem 3. Adrain C., Creagh E.M., Martin S.J. 2001. Apoptosis-associated release of Smac/DIABLO from mitochondria requires active caspases and is blocked by Bcl-2. EMBO J 20(23):6627-6636.4. Wu G., Chai J., Suber T.L., Wu J.W., Du C., Wang X., Shi Y. 2000. Structural basis of IAP recognition by Smac/DIABLO. Nature 408(6815):1008-1012.5. Liu Z., Sun C., Olejniczak E.T., Meadows R.P., Betz S.F., Oost T., Herrmann J., Wu J.C., Fesik S.W. 2000. Structural basis for binding of Smac/DIABLO to the XIAP BIR3 domain. Nature 408(6815):1004-1008.

Small volumes of anti-Smac/Diablo antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS440042 is an antibody targeting Smac/Diablo. Also known as Smac/Diablo (C17) Antibody. Reactive in Human. Host: Rabbit.