Human CCL19 Active Protein – Cat# MBS444010

Migration Assay: Cells expressing recombinant CCR7 were assayed for migration through a transwell filter at various concentrations of MIP-3beta. Responses are expressed as the % of total input cells.

CCL19 Human Active Protein

Name: CCL19 Active Protein; Human; MyBioSource
Brand: MyBioSource
SKU: MBS444010
Price: 175 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS444010

Macrophage inflammatory protein-3-beta (MIP3beta) (CCL19), also known as EBI1 ligand chemokine (ELC), directs chemotaxis of dendritic cells, and certain B- and T- lymphocytes, but not monocytes or granulocytes. It is constituitively expressed in thymus and… More details

$175 / 0.005 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

CCL19, Active Protein

Full Product Name

Human CCL19 (MIP-3beta)

Product Gene Name

CCL19 active protein

Product Synonym Names

CCL19 (MIP-3beta)

Sequence

GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPW VERIIQRLQR TSAKMKRRSS

Host

E Coli derived

Form/Format

Lyophilized

Purity/Purification

> 97% by SDS PAGE

Preparation and Storage

Avoid repeated freeze-thaw cycles
12 months from date of receipt, -20 to -70 degree C as supplied.
1 month, 2 to 8 degree C under sterile conditions after reconstitution.
3 months, -20 to -70 degree C under sterile conditions after reconstitution.

Matching Pairs

Unconjugated Antibody: CCL19 (MBS444010)
Biotin Conjugated Antibody: CCL19 (MBS444013)

Molecular Weight

Actual Molecular Mass: Mass Spec data confirmed expected mass of 8.800 kDa
Predicted Molecular Mass: 8.800 kDa

Sequence Positions

22-98

3D Structure

ModBase 3D Structure for P99731

Active Protein Note

Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.

Reconstitution

Spin sample prior to reconstitution. Recommended at 100mug/mL in sterile water

Carrier Protein

None

Activity

EC50 =7.2nM determined by migration assay with cells expressing recombinant CCR7

Extinction Coefficient

8,730 M-1 cm-1

Endotoxin Level

<0.01 EU per 1mug of the protein by the LAL method

Modification

None

UNSPSC Code

12352202

Product References and Citations for CCL19 active protein

1. "Molecular Cloning of a Novel Human CC Chemokine EBI1-ligand Chemokine That Is a Specific Functional Ligand for EBI1, CCR7" Yoshida R., Imai T., Hieshima K., Kusuda J., Baba M., Kitaura M., Nishimura M., Kakizaki M., Nomiyama H., Yoshie O.J Biol Chem 272:13803-13809 (1997)
2. "CK beta-11/macrophage inflammatory protein-3 beta/EBI1-ligand chemokine is an efficacious chemoattractant for T and B cells."Kim C.H., Pelus L.M., White J.R., Applebaum E., Johanson K., Broxmeyer H.E. J. Immunol. 160:2418-2424(1998)
3. "Homeostatic chemokines CCL19 and CCL21 promote inflammation in human immunodeficiency virus-infected patients with ongoing viral replication." Damås J.K., Landrø L., Fevang B., Heggelund L., Tjønnfjord G.E., Fløisand Y., Halvorsen B., Frøland S.S., Aukrust P. Clin Exp Immunol. 157:400-7 (2009)

Other Notes

Small volumes of CCL19 active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CCL19 purchase

MBS444010 is an active protein targeting CCL19. Also known as Human CCL19 (MIP-3beta). Host: E Coli derived.

Additional Details

NCBI/Uniprot data below describe general gene information for CCL19. It may not necessarily be applicable to this product.

UniProt Primary Accession #

P99731

Protein Family

C-C motif chemokine

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.