• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

ZO-1 Antibody – Goat Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS448025
13 units are in stock for immediate shipment. Please contact us for more details.

Goat polyclonal to TJPl tight Junction protein 1 (TJP1). TJPl is located on a cytoplasmic membrane surface of intercellular tight junctions and may be involved in signal transduction at cell-cell junctions. More details

$405 / 0.6 mg +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
ZO-1, Polyclonal Antibody
Full Product Name
ZO-1 Polyclonal Antibody
Product Gene Name
anti-ZO-1 antibody
Product Synonym Names
ZO-1 and zona occludens protein 1 antibody
Clonality
Polyclonal
Host
Goat
Species Reactivity
Reacts against Human, Rat, Mouse, Canine and Monkey proteins.
Form/Format
Polyclonal antibody supplied as a 200 ul (3 mg/ml) aliquot in PBS, 20% glycerol and 0.05% sodium azide.
Purity/Purification
Epitope-affinity purified from goat antiserum
Specificity
Detects endogenous levels of total ZO-1 by Western blot in whole cell and tissue lysates.
Preparation and Storage
For continuous use, store at 2-8 degree C for one-two days. For extended storage, store in -20 degree C freezer. Working dilution samples should be discarded if not used within 12 hours.
Chromosome Location
Chromosome: 7; NC_000073.6 (65296165..65527807, complement). Location: 7 C; 7 35.02 cM
3D Structure
ModBase 3D Structure for P39447
Immunogen
Purified recombinant peptide derived from within residues 1580 aa to the C-terminus of human ZO-1 produced in E. coli.
Applications Tested/Suitable for anti-ZO-1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC) - Frozen/Paraffin
Application Notes for anti-ZO-1 antibody
Western blot: 1:500-1:2,000
lmmunofluorescence: 1:50-1:250
Immunohistochemistry (paraffin): 1:200-1:1000
Immunohistochemistry (frozen): 1:200-1:1000

Special instructions: The antibody solution should be gently mixed before use. Avoid freeze/thaw cycles..

Reactivity Chart of anti-ZO-1 antibody

Goat Anti-ZO-1 Polyclonal Antibody – Cat# MBS448025

Immunoperoxidase of anti-ZO-1 antibody

Immunoperoxidase of polyclonal antibody to ZO-1 (1/200) on paraformaldehyde-fixed paraffin-embedded mouse kidney.
Goat Anti-ZO-1 Polyclonal Antibody, Immunoperoxidase – Cat# MBS448025

Western Blot of anti-ZO-1 antibody

Anti-ZO1 Ab at 1/2,500 dilution; 50ug of total protein per lane; rabbit polyclonal to goat IgG (HRP) at 1/10,000 dilution.
Goat Anti-ZO-1 Polyclonal Antibody, Western Blot – Cat# MBS448025

Immunofluorescence (IF) of anti-ZO-1 antibody

Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.
Goat Anti-ZO-1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS448025

Immunofluorescence (IF) of anti-ZO-1 antibody

Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.
Goat Anti-ZO-1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS448025

Immunofluorescence (IF) of anti-ZO-1 antibody

Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.
Goat Anti-ZO-1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS448025

Immunofluorescence (IF) of anti-ZO-1 antibody

Immunofluorescence of primary RPE cells with Z01 Ab at 1/100 dilution.
Goat Anti-ZO-1 Polyclonal Antibody, Immunofluorescence (IF) – Cat# MBS448025

Immunofluorescence of anti-ZO-1 antibody

Immunofluorescence of primary RPE cells with ZO1 Ab at 1/100 dilution.
Goat Anti-ZO-1 Polyclonal Antibody, Immunofluorescence – Cat# MBS448025

Immunohistochemistry (IHC) of anti-ZO-1 antibody

IHC of human pancreas using Z01 antibody and FFPE tissue after heat-induced antigen retrieval Anti-Z01 Ab at 1:750/DAB detection.
Goat Anti-ZO-1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS448025

Immunohistochemistry (IHC) of anti-ZO-1 antibody

IHC of human pancreas using Z01 antibody and FFPE tissue after heat-induced antigen retrieval Anti-Z01 Ab at 1:750/DAB detection.
Goat Anti-ZO-1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS448025

Immunohistochemistry (IHC) of anti-ZO-1 antibody

IHC of Mouse kidney using Z01 antibody and FFPE tissue after heat-induced retrieval. Anti-Z01 Ab at 1:750/DAB detection.
Goat Anti-ZO-1 Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS448025

Other Notes

Small volumes of anti-ZO-1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ZO-1 purchase

MBS448025 is an antibody targeting ZO-1. Also known as ZO-1 Polyclonal Antibody. Reactive in Reacts against Human, Rat, Mouse, Canine and Monkey proteins.. Host: Goat.

Additional Details

NCBI/Uniprot data below describe general gene information for ZO-1. It may not necessarily be applicable to this product.

NCBI GI #
303710
NCBI GeneID
21872
NCBI Accession #
BAA03274.1
UniProt Primary Accession #
P39447
UniProt Secondary Accession #
E9QK00
UniProt Related Accession #
P39447
NCBI Official Full Name
ZO-1
NCBI Official Synonym Full Names
tight junction protein 1
NCBI Official Symbol
Tjp1
NCBI Official Synonym Symbols
ZO1
UniProt Protein Name
Tight junction protein ZO-1
UniProt Synonym Protein Names
Tight junction protein 1; Zona occludens protein 1; Zonula occludens protein 1
UniProt Gene Name
Tjp1
UniProt Synonym Gene Names
Zo1
UniProt Entry Name
ZO1_MOUSE
NCBI Protein Information
tight junction protein ZO-1; zona occludens protein 1; zonula occludens protein 1

UniProt Comments for ZO-1

Function: The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells

By similarity.

Subunit structure: Homodimer, and heterodimer with TJP2/ZO-2 and TJP3/ZO-3. Interacts with CGN/cingulin, CALM, CXADR, GJD3, HSPA4 and UBN1. Interacts (via ZU5 domain) with CDC42BPB. Interacts (via PDZ domain) with GJA1

By similarity. Interacts with OCLN, claudins, GJA12 and KIRREL1. Interacts with BVES (via the C-terminus cytoplasmic tail). Interacts (via ZU5 domain) with MYZAP. Interacts (via PDZ domains) with ANKRD2

By similarity. Ref.3 Ref.4 Ref.5 Ref.12

Subcellular location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

By similarity. Cell junction › tight junction

By similarity. Cell junction › gap junction. Cytoplasm › myofibril › sarcomere › I band. Note: Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact. Detected at the leading edge of migrating and wounded cells

By similarity. Ref.12

Domain: The second PDZ domain mediates interaction with GJA12. Ref.4

Post-translational modification: Phosphorylated. Dephosphorylated by PTPRJ

By similarity.

Sequence similarities: Belongs to the MAGUK family.Contains 1 guanylate kinase-like domain.Contains 3 PDZ (DHR) domains.Contains 1 SH3 domain.Contains 1 ZU5 domain.
Show more

Research Articles on ZO-1

1. The JAM-A and ZO-1 genes were highly expressed in all the tested tissues.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-ZO-1 antibody

 Reference Product  PubMed Publications
 Ocln antibody  >513 publications with ZO-1 and Ocln
 Cldn5 antibody  >114 publications with ZO-1 and Cldn5
 Cldn4 antibody  >45 publications with ZO-1 and Cldn4
 F11r antibody  >24 publications with ZO-1 and F11r
 Cgn antibody  >18 publications with ZO-1 and Cgn
 Gja1 antibody  >11 publications with ZO-1 and Gja1
 Jam2 antibody  >1 publications with ZO-1 and Jam2

Pathways associated with anti-ZO-1 antibody

 Products by Pathway  Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Adherens Junction Pathway antibodies  Adherens Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Pathway antibodies  Gap Junction Pathway Diagram
 Gap Junction Trafficking And Regulation Pathway antibodies  Gap Junction Trafficking And Regulation Pathway Diagram
 Membrane Trafficking Pathway antibodies  Membrane Trafficking Pathway Diagram
 PodNet: Protein-protein Interactions In The Podocyte Pathway antibodies  PodNet: Protein-protein Interactions In The Podocyte Pathway Diagram
 Regulation Of Gap Junction Activity Pathway antibodies  Regulation Of Gap Junction Activity Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram
 Salmonella Infection Pathway antibodies  Salmonella Infection Pathway Diagram

Diseases associated with anti-ZO-1 antibody

 Disease Name  Pubmed Publications
 Inflammation Antibodies  >181 publications with ZO-1 and Inflammation
 Neoplasms Antibodies  >152 publications with ZO-1 and Neoplasms
 Disease Models, Animal Antibodies  >141 publications with ZO-1 and Disease Models, Animal
 Nervous System Diseases Antibodies  >128 publications with ZO-1 and Nervous System Diseases
 Necrosis Antibodies  >116 publications with ZO-1 and Necrosis
 Cardiovascular Diseases Antibodies  >101 publications with ZO-1 and Cardiovascular Diseases
 Edema Antibodies  >63 publications with ZO-1 and Edema
 Kidney Diseases Antibodies  >62 publications with ZO-1 and Kidney Diseases
 Fibrosis Antibodies  >38 publications with ZO-1 and Fibrosis
 Liver Diseases Antibodies  >38 publications with ZO-1 and Liver Diseases

Organs/Tissues associated with anti-ZO-1 antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >330 publications with ZO-1 and Brain
 Kidney Antibodies  >275 publications with ZO-1 and Kidney
 Eye Antibodies  >254 publications with ZO-1 and Eye
 Intestine Antibodies  >238 publications with ZO-1 and Intestine
 Liver Antibodies  >135 publications with ZO-1 and Liver
 Muscle Antibodies  >120 publications with ZO-1 and Muscle
 Embryonic Tissue Antibodies  >110 publications with ZO-1 and Embryonic Tissue
 Lung Antibodies  >92 publications with ZO-1 and Lung
 Testis Antibodies  >69 publications with ZO-1 and Testis
 Heart Antibodies  >68 publications with ZO-1 and Heart
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000