Glutamate Cysteine Ligase Catalytic Subunit (GCLC) ELISA Kit for Rabbit

Name: Rabbit GCLC ELISA Kit; Glutamate Cysteine Ligase Catalytic Subunit; MyBioSource
Brand: MyBioSource
SKU: MBS044980
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS044980

Background: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used to determine the level of GCLC (hereafter termed "analyte") in undiluted original… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Glutamate Cysteine Ligase Catalytic Subunit (GCLC), ELISA Kit

Full Product Name

Rabbit Glutamate Cysteine Ligase Catalytic Subunit ELISA Kit

Product Gene Name

GCLC elisa kit

Species Reactivity

Rabbit

Assay Type

Quantitative Sandwich

Detection Range

0.625ng/ml-20ng/ml

Sensitivity

0.1ng/ml

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

72,766 Da

Chromosome Location

Chromosome: 6; NC_000006.11 (53362139..53409927, complement). Location: 6p12

OMIM

230450

3D Structure

ModBase 3D Structure for P48506

Other Notes

Small volumes of GCLC elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for GCLC purchase

MBS044980 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Glutamate Cysteine Ligase Catalytic Subunit (GCLC) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing GCLC. The ELISA analytical biochemical technique of the MBS044980 kit is based on GCLC antibody-GCLC antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect GCLC antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, GCLC. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for GCLC. It may not necessarily be applicable to this product.

NCBI GI #

308199423

NCBI GeneID

2729

NCBI Accession #

NP_001184044.1

UniProt Primary Accession #

P48506

UniProt Secondary Accession #

Q14399

UniProt Related Accession #

P48506

GenBank Nucleotide #

NM_001197115.1

NCBI Official Full Name

glutamate--cysteine ligase catalytic subunit isoform b

NCBI Official Synonym Full Names

glutamate-cysteine ligase, catalytic subunit

NCBI Official Symbol

GCLC

NCBI Official Synonym Symbols

GCL; GCS; GLCL; GLCLC

UniProt Protein Name

Glutamate--cysteine ligase catalytic subunit

UniProt Synonym Protein Names

GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase

Protein Family

Glutamate--cysteine ligase

UniProt Gene Name

GCLC

UniProt Synonym Gene Names

GLCL; GLCLC

UniProt Entry Name

GSH1_HUMAN

NCBI Protein Information

glutamate--cysteine ligase catalytic subunit; gamma-ECS; GCS heavy chain; gamma-glutamylcysteine synthetase

NCBI Summary for GCLC

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]

UniProt Comments for GCLC

GCLC: Defects in GCLC are the cause of hemolytic anemia (HAGGSD). Belongs to the glutamate--cysteine ligase type 3 family.

Protein type: EC 6.3.2.2; Ligase; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 6p12

Cellular Component: glutamate-cysteine ligase complex; cytosol

Molecular Function: glutamate binding; protein heterodimerization activity; glutamate-cysteine ligase activity; magnesium ion binding; ADP binding; coenzyme binding; ATP binding

Biological Process: glutamate metabolic process; response to arsenic; response to hormone stimulus; L-ascorbic acid metabolic process; response to nitrosative stress; cysteine metabolic process; apoptotic mitochondrial changes; response to heat; cell redox homeostasis; sulfur amino acid metabolic process; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; xenobiotic metabolic process; glutathione biosynthetic process; negative regulation of protein ubiquitination; response to oxidative stress; negative regulation of neuron apoptosis; regulation of mitochondrial depolarization; regulation of blood vessel size; negative regulation of transcription, DNA-dependent; negative regulation of apoptosis

Disease: Gamma-glutamylcysteine Synthetase Deficiency, Hemolytic Anemia Due To; Myocardial Infarction, Susceptibility To

Research Articles on GCLC

1. Genistein up-regulated HO-1 and Glutamate-Cysteine Ligase expression through the EKR1/2 and PKC /Nrf2 pathways during oxidative stress.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with GCLC elisa kit

Reference Product	PubMed Publications
GCLM elisa kit	>147 publications with GCLC and GCLM
GSS elisa kit	>7 publications with GCLC and GSS
GGT1 elisa kit	>1 publications with GCLC and GGT1

Pathways associated with GCLC elisa kit

Products by Pathway	Pathway Diagram
Biological Oxidations Pathway antibodies	Biological Oxidations Pathway Diagram
Glutathione Biosynthesis, Glutamate => Glutathione Pathway antibodies	Glutathione Biosynthesis, Glutamate => Glutathione Pathway Diagram
Glutathione Biosynthesis, Glutamate => Glutathione Pathway antibodies	Glutathione Biosynthesis, Glutamate => Glutathione Pathway Diagram
Glutathione Conjugation Pathway antibodies	Glutathione Conjugation Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Metabolism Pathway antibodies	Glutathione Metabolism Pathway Diagram
Glutathione Synthesis And Recycling Pathway antibodies	Glutathione Synthesis And Recycling Pathway Diagram
Keap1-Nrf2 Pathway antibodies	Keap1-Nrf2 Pathway Diagram
Metabolism Pathway antibodies	Metabolism Pathway Diagram

Diseases associated with GCLC elisa kit

Disease Name	Pubmed Publications
Drug Toxicity Antibodies	>61 publications with GCLC and Drug Toxicity
Liver Diseases Antibodies	>40 publications with GCLC and Liver Diseases
Neoplasms Antibodies	>27 publications with GCLC and Neoplasms
Necrosis Antibodies	>24 publications with GCLC and Necrosis
Inflammation Antibodies	>19 publications with GCLC and Inflammation
Cardiovascular Diseases Antibodies	>17 publications with GCLC and Cardiovascular Diseases
Brain Diseases Antibodies	>14 publications with GCLC and Brain Diseases
Drug-Induced Liver Injury Antibodies	>13 publications with GCLC and Drug-Induced Liver Injury
Nervous System Diseases Antibodies	>10 publications with GCLC and Nervous System Diseases
Fibrosis Antibodies	>9 publications with GCLC and Fibrosis

Organs/Tissues associated with GCLC elisa kit

Organ/Tissue Name	Pubmed Publications
Liver Antibodies	>83 publications with GCLC and Liver
Blood Antibodies	>47 publications with GCLC and Blood
Lung Antibodies	>44 publications with GCLC and Lung
Brain Antibodies	>27 publications with GCLC and Brain
Kidney Antibodies	>19 publications with GCLC and Kidney
Muscle Antibodies	>13 publications with GCLC and Muscle
Skin Antibodies	>8 publications with GCLC and Skin
Pancreas Antibodies	>7 publications with GCLC and Pancreas
Nerve Antibodies	>7 publications with GCLC and Nerve
Ovary Antibodies	>7 publications with GCLC and Ovary