Poly A Specific Ribonuclease (PARN) ELISA Kit for Human

Name: Human PARN ELISA Kit; Poly A Specific Ribonuclease; MyBioSource
Brand: MyBioSource
SKU: MBS453699
Price: 520 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS453699

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PARN in human tissue homogenates and other biological fluids.Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an… More details

$520 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Poly A Specific Ribonuclease (PARN), ELISA Kit

Full Product Name

Human Poly A Specific Ribonuclease (PARN) ELISA Kit

Product Gene Name

PARN elisa kit

Product Synonym Names

DAN Deadenylation Nuclease Deadenylating nuclease Polyadenylate-specific ribonuclease

Species Reactivity

Human

Assay Type

Sandwich

Samples

Tissue homogenates and other biological fluids.

Detection Range

0.781-50ng/mL

Sensitivity

0.27ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of PARN. No significant cross-reactivity or interference between PARN and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PARN were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PARN were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

52,989 Da

OMIM

AJ005698 mRNA

3D Structure

ModBase 3D Structure for O95453

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of PARN elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for PARN purchase

MBS453699 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Poly A Specific Ribonuclease (PARN) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing PARN. The ELISA analytical biochemical technique of the MBS453699 kit is based on PARN antibody-PARN antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect PARN antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, PARN. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for PARN. It may not necessarily be applicable to this product.

NCBI GI #

197333695

NCBI GeneID

5073

NCBI Accession #

NP_001127949.1

UniProt Primary Accession #

O95453

UniProt Secondary Accession #

B2RCB3; B4DDG8; B4DSB0; B4DWR4; B4E1H6

UniProt Related Accession #

O95453

GenBank Nucleotide #

NM_001134477.2

NCBI Official Full Name

poly(A)-specific ribonuclease PARN isoform 2

NCBI Official Synonym Full Names

poly(A)-specific ribonuclease

NCBI Official Symbol

PARN

NCBI Official Synonym Symbols

DAN; DKCB6; PFBMFT4

UniProt Protein Name

Poly(A)-specific ribonuclease PARN

UniProt Synonym Protein Names

Deadenylating nuclease; Deadenylation nuclease; Polyadenylate-specific ribonuclease

Protein Family

Poly(A)-specific ribonuclease

UniProt Gene Name

PARN

UniProt Synonym Gene Names

DAN

NCBI Protein Information

poly(A)-specific ribonuclease PARN

NCBI Summary for PARN

The protein encoded by this gene is a 3'-exoribonuclease, with similarity to the RNase D family of 3'-exonucleases. It prefers poly(A) as the substrate, hence, efficiently degrades poly(A) tails of mRNAs. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs. This protein is also involved in silencing of certain maternal mRNAs during oocyte maturation and early embryonic development, as well as in nonsense-mediated decay (NMD) of mRNAs that contain premature stop codons. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

UniProt Comments for PARN

3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization.

Research Articles on PARN

1. we found a polyadenylation-dependent 3' end maturation pathway for the human telomerase RNA that relies on the nuclear poly(A)-binding protein PABPN1 and the poly(A)-specific RNase PARN.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with PARN elisa kit

Reference Product	PubMed Publications
EIF4E elisa kit	>7 publications with PARN and EIF4E
DCP2 elisa kit	>3 publications with PARN and DCP2
KHSRP elisa kit	>2 publications with PARN and KHSRP

Pathways associated with PARN elisa kit

Products by Pathway	Pathway Diagram
ATF4 Activates Genes Pathway antibodies	ATF4 Activates Genes Pathway Diagram
Deadenylation Of MRNA Pathway antibodies	Deadenylation Of MRNA Pathway Diagram
Deadenylation-dependent MRNA Decay Pathway antibodies	Deadenylation-dependent MRNA Decay Pathway Diagram
Gene Expression Pathway antibodies	Gene Expression Pathway Diagram
KSRP (KHSRP) Binds And Destabilizes MRNA Pathway antibodies	KSRP (KHSRP) Binds And Destabilizes MRNA Pathway Diagram
Lung Fibrosis Pathway antibodies	Lung Fibrosis Pathway Diagram
Metabolism Of Proteins Pathway antibodies	Metabolism Of Proteins Pathway Diagram
PERK Regulates Gene Expression Pathway antibodies	PERK Regulates Gene Expression Pathway Diagram
RNA Degradation Pathway antibodies	RNA Degradation Pathway Diagram
RNA Degradation Pathway antibodies	RNA Degradation Pathway Diagram

Diseases associated with PARN elisa kit

Disease Name	Pubmed Publications
Idiopathic Pulmonary Fibrosis Antibodies	>6 publications with PARN and Idiopathic Pulmonary Fibrosis
Necrosis Antibodies	>3 publications with PARN and Necrosis
Nervous System Diseases Antibodies	>2 publications with PARN and Nervous System Diseases
Inflammation Antibodies	>2 publications with PARN and Inflammation
Heart Diseases Antibodies	>1 publications with PARN and Heart Diseases
Infertility, Male Antibodies	>1 publications with PARN and Infertility, Male
Fetal Growth Retardation Antibodies	>1 publications with PARN and Fetal Growth Retardation
Adenocarcinoma Antibodies	>1 publications with PARN and Adenocarcinoma
Disease Models, Animal Antibodies	>1 publications with PARN and Disease Models, Animal
Learning Disorders Antibodies	>1 publications with PARN and Learning Disorders

Organs/Tissues associated with PARN elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>9 publications with PARN and Blood
Lung Antibodies	>7 publications with PARN and Lung
Bone Antibodies	>5 publications with PARN and Bone
Bone Marrow Antibodies	>4 publications with PARN and Bone Marrow
Muscle Antibodies	>3 publications with PARN and Muscle
Brain Antibodies	>3 publications with PARN and Brain
Skin Antibodies	>2 publications with PARN and Skin
Eye Antibodies	>2 publications with PARN and Eye
Connective Tissue Antibodies	>2 publications with PARN and Connective Tissue
Ovary Antibodies	>2 publications with PARN and Ovary