Htra Serine Peptidase 1 (HTRA1) ELISA Kit for Human

Name: Human HTRA1 ELISA Kit; Htra Serine Peptidase 1; MyBioSource
Brand: MyBioSource
SKU: MBS454847
Price: 555 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS454847

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to HTRA1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details

$555 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

HtrA Serine Peptidase 1 (HTRA1), ELISA Kit

Full Product Name

Human HtrA Serine Peptidase 1 (HTRA1) ELISA Kit

Product Gene Name

HTRA1 elisa kit

Product Synonym Names

HtrA; L56; ORF480; PRSS11; HtrA Serine Peptidase 1; Protease,Serine,11; IGFBP5-Protease; High-temperature requirement A serine peptidase 1

Species Reactivity

Human

Assay Type

Quantitative Sandwich

Samples

Human serum, plasma or other biological fluids

Detection Range

31.2-2000pg/mL

Sensitivity

< 13.2pg/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of HTRA1. No significant cross-reactivity or interference between HTRA1 and analogues was observed.

Preparation and Storage

For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level HTRA1 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level HTRA1 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

51,287 Da

OMIM

Y07921 mRNA

3D Structure

ModBase 3D Structure for Q92743

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of HTRA1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for HTRA1 purchase

MBS454847 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the HtrA Serine Peptidase 1 (HTRA1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing HTRA1. The ELISA analytical biochemical technique of the MBS454847 kit is based on HTRA1 antibody-HTRA1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect HTRA1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, HTRA1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for HTRA1. It may not necessarily be applicable to this product.

NCBI GI #

4506141

NCBI GeneID

5654

NCBI Accession #

NP_002766.1

UniProt Primary Accession #

Q92743

UniProt Secondary Accession #

Q9UNS5; D3DRE4

UniProt Related Accession #

Q92743

GenBank Nucleotide #

NM_002775.4

NCBI Official Full Name

serine protease HTRA1

NCBI Official Synonym Full Names

HtrA serine peptidase 1

NCBI Official Symbol

HTRA1

NCBI Official Synonym Symbols

L56; HtrA; ARMD7; ORF480; PRSS11; CARASIL; CADASIL2

UniProt Protein Name

Serine protease HTRA1

UniProt Synonym Protein Names

High-temperature requirement A serine peptidase 1; L56; Serine protease 11

Protein Family

Serine protease

UniProt Gene Name

HTRA1

UniProt Synonym Gene Names

HTRA; PRSS11

NCBI Protein Information

serine protease HTRA1

NCBI Summary for HTRA1

This gene encodes a member of the trypsin family of serine proteases. This protein is a secreted enzyme that is proposed to regulate the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. It has also been suggested to be a regulator of cell growth. Variations in the promoter region of this gene are the cause of susceptibility to age-related macular degeneration type 7. [provided by RefSeq, Jul 2008]

UniProt Comments for HTRA1

Serine protease with a variety of targets, including extracellular matrix proteins such as fibronectin. HTRA1-generated fibronectin fragments further induce synovial cells to up-regulate MMP1 and MMP3 production. May also degrade proteoglycans, such as aggrecan, decorin and fibromodulin. Through cleavage of proteoglycans, may release soluble FGF-glycosaminoglycan complexes that promote the range and intensity of FGF signals in the extracellular space. Regulates the availability of insulin-like growth factors (IGFs) by cleaving IGF-binding proteins. Inhibits signaling mediated by TGF-beta family members. This activity requires the integrity of the catalytic site, although it is unclear whether TGF-beta proteins are themselves degraded. By acting on TGF-beta signaling, may regulate many physiological processes, including retinal angiogenesis and neuronal survival and maturation during development. Intracellularly, degrades TSC2, leading to the activation of TSC2 downstream targets.

Research Articles on HTRA1

1. correlation between TGFb1 and pSmad2 as well as between HtrA1 and TGFb1 and the very significant increase of Ki67 in stromal compartment of eutopic endometrium suggest a possible involvement of HtrA1 in the pathogenesis of endometriosis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with HTRA1 elisa kit

Reference Product	PubMed Publications
ARMS2 elisa kit	>150 publications with HTRA1 and ARMS2
EGFR elisa kit	>3 publications with HTRA1 and EGFR
XIAP elisa kit	>2 publications with HTRA1 and XIAP
TSC2 elisa kit	>1 publications with HTRA1 and TSC2

Pathways associated with HTRA1 elisa kit

Products by Pathway	Pathway Diagram
Degradation Of The Extracellular Matrix Pathway antibodies	Degradation Of The Extracellular Matrix Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram

Diseases associated with HTRA1 elisa kit

Disease Name	Pubmed Publications
Macular Degeneration, Age-Related, 7 Antibodies	>78 publications with HTRA1 and Macular Degeneration, Age-Related, 7
Cardiovascular Diseases Antibodies	>51 publications with HTRA1 and Cardiovascular Diseases
Disease Models, Animal Antibodies	>20 publications with HTRA1 and Disease Models, Animal
Inflammation Antibodies	>18 publications with HTRA1 and Inflammation
Carcinoma Antibodies	>16 publications with HTRA1 and Carcinoma
Adenocarcinoma Antibodies	>11 publications with HTRA1 and Adenocarcinoma
Neoplasms, Experimental Antibodies	>10 publications with HTRA1 and Neoplasms, Experimental
Vision Disorders Antibodies	>9 publications with HTRA1 and Vision Disorders
Hypertension Antibodies	>8 publications with HTRA1 and Hypertension
Breast Neoplasms Antibodies	>8 publications with HTRA1 and Breast Neoplasms

Organs/Tissues associated with HTRA1 elisa kit

Organ/Tissue Name	Pubmed Publications
Eye Antibodies	>160 publications with HTRA1 and Eye
Vascular Antibodies	>68 publications with HTRA1 and Vascular
Blood Antibodies	>55 publications with HTRA1 and Blood
Brain Antibodies	>33 publications with HTRA1 and Brain
Bone Antibodies	>31 publications with HTRA1 and Bone
Ear Antibodies	>25 publications with HTRA1 and Ear
Placenta Antibodies	>25 publications with HTRA1 and Placenta
Connective Tissue Antibodies	>15 publications with HTRA1 and Connective Tissue
Embryonic Tissue Antibodies	>14 publications with HTRA1 and Embryonic Tissue
Nerve Antibodies	>12 publications with HTRA1 and Nerve