Bone Morphogenetic Protein 10 (BMP10) ELISA Kit for Mouse

Name: Mouse BMP10 ELISA Kit; Bone Morphogenetic Protein 10; MyBioSource
Brand: MyBioSource
SKU: MBS457928
Price: 550 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS457928

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BMP10. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to… More details

$550 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Bone Morphogenetic Protein 10 (BMP10), ELISA Kit

Full Product Name

Mouse Bone Morphogenetic Protein 10 (BMP10) ELISA Kit

Product Gene Name

BMP10 elisa kit

Species Reactivity

Mouse

Assay Type

Quantitative Sandwich

Samples

Mouse serum, plasma, tissue homogenates or other biological fluids.

Detection Range

0.156-10ng/mL

Sensitivity

< 0.057ng/mL

Specificity

This assay has high sensitivity and excellent specificity for detection of BMP10. No significant cross-reactivity or interference between BMP10 and analogues was observed.

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Inter-assay Precision

Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BMP10 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%

Intra-assay Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BMP10 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%

Molecular Weight

47,888 Da

3D Structure

ModBase 3D Structure for Q9R229

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of BMP10 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BMP10 purchase

MBS457928 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Bone Morphogenetic Protein 10 (BMP10) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing BMP10. The ELISA analytical biochemical technique of the MBS457928 kit is based on BMP10 antibody-BMP10 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect BMP10 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, BMP10. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for BMP10. It may not necessarily be applicable to this product.

NCBI GI #

157837977

NCBI GeneID

12154

NCBI Accession #

NP_033886.2

UniProt Primary Accession #

Q9R229

UniProt Secondary Accession #

Q9Z1V8; A6H6S5

UniProt Related Accession #

Q9R229

GenBank Nucleotide #

NM_009756.3

NCBI Official Full Name

bone morphogenetic protein 10 preproprotein

NCBI Official Synonym Full Names

bone morphogenetic protein 10

NCBI Official Symbol

Bmp10

NCBI Official Synonym Symbols

b2b2711Clo

UniProt Protein Name

Bone morphogenetic protein 10

Protein Family

Bone morphogenetic protein

UniProt Gene Name

Bmp10

UniProt Synonym Gene Names

BMP-10

NCBI Protein Information

bone morphogenetic protein 10

NCBI Summary for BMP10

This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate the mature protein, which binds to the activin receptor-like kinase 1 (ALK1) and plays important roles in cardiovascular development including cardiomyocyte proliferation and regulation of heart size, closure of the ductus arteriosus, angiogenesis and ventricular trabeculation. Homozygous knockout mice for this gene exhibit impaired heart development and embryonic lethality. [provided by RefSeq, Aug 2016]

UniProt Comments for BMP10

Required for maintaining the proliferative activity of embryonic cardiomyocytes by preventing premature activation of the negative cell cycle regulator CDKN1C/p57KIP and maintaining the required expression levels of cardiogenic factors such as MEF2C and NKX2-5. Acts as a ligand for ACVRL1/ALK1, BMPR1A/ALK3 and BMPR1B/ALK6, leading to activation of SMAD1, SMAD5 and SMAD8 transcription factors. Inhibits endothelial cell migration and growth. May reduce cell migration and cell matrix adhesion in breast cancer cell lines ().

Research Articles on BMP10

1. CV2 binds directly to BMP10, as determined by co-immunoprecipitation, and inhibits BMP10 from initiating SMAD signaling, as determined by luciferase reporter gene assays. BMP10 and CV2 have important roles in coordinating cardiomyogenesis in progenitor cells.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with BMP10 elisa kit

Reference Product	PubMed Publications
Bmpr2 elisa kit	>14 publications with BMP10 and Bmpr2
Acvrl1 elisa kit	>12 publications with BMP10 and Acvrl1
Bmp2 elisa kit	>9 publications with BMP10 and Bmp2
Nkx2-5 elisa kit	>8 publications with BMP10 and Nkx2-5
Bmp4 elisa kit	>6 publications with BMP10 and Bmp4
Bmp7 elisa kit	>5 publications with BMP10 and Bmp7
Gdf5 elisa kit	>2 publications with BMP10 and Gdf5
Cdkn1c elisa kit	>1 publications with BMP10 and Cdkn1c

Pathways associated with BMP10 elisa kit

Products by Pathway	Pathway Diagram
Elastic Fibre Formation Pathway antibodies	Elastic Fibre Formation Pathway Diagram
Extracellular Matrix Organization Pathway antibodies	Extracellular Matrix Organization Pathway Diagram
Heart Development Pathway antibodies	Heart Development Pathway Diagram
Molecules Associated With Elastic Fibres Pathway antibodies	Molecules Associated With Elastic Fibres Pathway Diagram

Diseases associated with BMP10 elisa kit

Disease Name	Pubmed Publications
Vascular Diseases Antibodies	>8 publications with BMP10 and Vascular Diseases
Hypertrophy Antibodies	>4 publications with BMP10 and Hypertrophy
Vascular Malformations Antibodies	>4 publications with BMP10 and Vascular Malformations
Cardiomegaly Antibodies	>4 publications with BMP10 and Cardiomegaly
Fibrosis Antibodies	>3 publications with BMP10 and Fibrosis
Breast Neoplasms Antibodies	>2 publications with BMP10 and Breast Neoplasms
Inflammation Antibodies	>2 publications with BMP10 and Inflammation
Abnormalities, Multiple Antibodies	>1 publications with BMP10 and Abnormalities, Multiple
Neoplasm Metastasis Antibodies	>1 publications with BMP10 and Neoplasm Metastasis
Edema Antibodies	>1 publications with BMP10 and Edema

Organs/Tissues associated with BMP10 elisa kit

Organ/Tissue Name	Pubmed Publications
Heart Antibodies	>28 publications with BMP10 and Heart