Deoxyribonuclease 1 (DNASE1) ELISA Kit for Human

Name: Human DNASE1 ELISA Kit; Deoxyribonuclease 1; MyBioSource
Brand: MyBioSource
SKU: MBS458891
Price: 525 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS458891

Test Principle: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DNASE1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DNASE1.… More details

$525 / 48 Tests +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Request Current Manual

Product Overview

Online Datasheet in PDF

Product Name

Deoxyribonuclease I (DNASE1), ELISA Kit

Full Product Name

Human Deoxyribonuclease I (DNASE1) ELISA Kit

Product Gene Name

DNASE1 elisa kit

Product Synonym Names

DNaseI; DNL1; DRNI; DNase-I; Dornase alfa

Species Reactivity

Human

Assay Type

Sandwich

Samples

Serum, Plasma, Urine, Saliva or Other Biological Fluids.

Detection Range

1.56-100ng/mL

Sensitivity

0.65ng/mL

Preparation and Storage

For unopened kits: All the reagents should be kept at 4 degree C upon receipt.
For opened kits: Once the kit is opened, the remaining reagents still need to be stored according to the above storage conditions. In addition, return the unused wells to the foil pouch containing the desiccant pack and reseal along entire edge of zip-seal.

Molecular Weight

19,771 Da

OMIM

125505

3D Structure

ModBase 3D Structure for P24855

RTU ELISA Kit's Advantage

The Ready-To-Use (RTU) Kits require fewer steps in setup and take less time to run. Detection Solutions are pre-diluted and total incubation time is shorter than many other ELISA Kits.

ISO Certification

Manufactured in an ISO 13485:2003 and EN ISO 13485:2012 Certified Laboratory.

Other Notes

Small volumes of DNASE1 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DNASE1 purchase

MBS458891 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Deoxyribonuclease I (DNASE1) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing DNASE1. The ELISA analytical biochemical technique of the MBS458891 kit is based on DNASE1 antibody-DNASE1 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect DNASE1 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, DNASE1. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for DNASE1. It may not necessarily be applicable to this product.

NCBI GI #

21361254

NCBI GeneID

1773

NCBI Accession #

NP_005214.2

UniProt Primary Accession #

P24855

UniProt Secondary Accession #

Q14UU9; Q14UV0; B4DV35

UniProt Related Accession #

P24855

GenBank Nucleotide #

NM_005223.3

NCBI Official Full Name

deoxyribonuclease-1

NCBI Official Synonym Full Names

deoxyribonuclease 1

NCBI Official Symbol

DNASE1

NCBI Official Synonym Symbols

DNL1; DRNI

UniProt Protein Name

Deoxyribonuclease-1

UniProt Synonym Protein Names

Deoxyribonuclease I; DNase I; INN: Dornase alfa

Protein Family

Deoxyribonuclease

UniProt Gene Name

DNASE1

UniProt Synonym Gene Names

DNase I

NCBI Protein Information

deoxyribonuclease-1

NCBI Summary for DNASE1

This gene encodes a member of the DNase family. This protein is stored in the zymogen granules of the nuclear envelope and functions by cleaving DNA in an endonucleolytic manner. At least six autosomal codominant alleles have been characterized, DNASE1*1 through DNASE1*6, and the sequence of DNASE1*2 represented in this record. Mutations in this gene have been associated with systemic lupus erythematosus (SLE), an autoimmune disease. A recombinant form of this protein is used to treat the one of the symptoms of cystic fibrosis by hydrolyzing the extracellular DNA in sputum and reducing its viscosity. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]

UniProt Comments for DNASE1

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs (PubMed:2251263, PubMed:11241278, PubMed:2277032). Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA (). Among other functions, seems to be involved in cell death by apoptosis (PubMed:11241278). Binds specifically to G-actin and blocks actin polymerization (). Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs) (). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation (). Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation ().

Research Articles on DNASE1

1. Genetic variants in DNase I hypersensitive sites play an important role in carcinogenesis. two novel single nucleotide polymorphisms rs12309362 (odds ratio = 0.64, P = 5.61 x 10(-6) ) and rs9970827 (odds ratio = 0.73, P = 7.23 x 10(-6) ) significantly associated with decreased risk of HCC.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with DNASE1 elisa kit

Reference Product	PubMed Publications
ACTA2 elisa kit	>1 publications with DNASE1 and ACTA2

Diseases associated with DNASE1 elisa kit

Disease Name	Pubmed Publications
Fibrosis Antibodies	>220 publications with DNASE1 and Fibrosis
Lupus Erythematosus, Systemic Antibodies	>28 publications with DNASE1 and Lupus Erythematosus, Systemic
Inflammation Antibodies	>25 publications with DNASE1 and Inflammation
Kidney Diseases Antibodies	>12 publications with DNASE1 and Kidney Diseases
Necrosis Antibodies	>10 publications with DNASE1 and Necrosis
Heart Defects, Congenital Antibodies	>6 publications with DNASE1 and Heart Defects, Congenital
Proteinuria Antibodies	>4 publications with DNASE1 and Proteinuria
Drug-Induced Liver Injury Antibodies	>3 publications with DNASE1 and Drug-Induced Liver Injury
Liver Diseases Antibodies	>3 publications with DNASE1 and Liver Diseases
Pain Antibodies	>3 publications with DNASE1 and Pain

Organs/Tissues associated with DNASE1 elisa kit

Organ/Tissue Name	Pubmed Publications
Lung Antibodies	>102 publications with DNASE1 and Lung
Blood Antibodies	>50 publications with DNASE1 and Blood
Kidney Antibodies	>15 publications with DNASE1 and Kidney
Heart Antibodies	>14 publications with DNASE1 and Heart
Liver Antibodies	>11 publications with DNASE1 and Liver
Muscle Antibodies	>5 publications with DNASE1 and Muscle
Eye Antibodies	>5 publications with DNASE1 and Eye
Skin Antibodies	>5 publications with DNASE1 and Skin
Bone Antibodies	>4 publications with DNASE1 and Bone
Vascular Antibodies	>3 publications with DNASE1 and Vascular