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DBF4 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460395

The human DBF4/ASK protein contains a putative site for phosphorylation by CDK, PEST protease cleavage sites, nuclear localization signals and a short-looped zinc finger-like domain. DBF4 interacts physically with CDC7 and MCM2 as a cyclin-like regulatory… More details

$355 / 0.1 mg +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
DBF4, Polyclonal Antibody
Full Product Name
Rabbit anti DBF4 Polyclonal Antibody
Product Gene Name
Product Synonym Names
DBF4; DBF4A
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Rat, Mouse
Form/Format
Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.
Purity/Purification
Purified by Epitope Affinity Purification
Specificity
This antibody recognizes ~77 kDa of DBF4 protein. It cross reacts to human, mouse and rat. The other species are not tested.
Preparation and Storage
The antibodies are stable when stored at -20°C to -70°C. The antibodies can be stored at 2°C-8°C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Molecular Weight
77.0
Preparation
A synthetic peptide corresponding to the N-terminus of DBF4 protein. This sequence is identical to human, rat and mouse.
Positive Control
Kidney Tissue
Cellular Location
Cell Membrane
Applications Tested/Suitable for anti-DBF4 antibody
ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)
Application Notes for anti-DBF4 antibody
WB: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
IP: 2-5 ug/ml
IHC: 2-10 ug/ml

Western Blot(WB) of anti-DBF4 antibody

Western Blot: The tissue extract derived from mouse spleen was immunoblotted by Rabbit anti DBF4 (Cat#MBS460395) at 1:500. An immunoreactive band is observed ~77 kDa.
Rabbit Anti-DBF4 Polyclonal Antibody – Cat# MBS460395

Product References and Citations for anti-DBF4 antibody

Lepke, M., Putter, V., Staib, C., Kneissl, M., Berger, C., Hoehn, K., Nanda, I., Schmid, M. and Grummt, F. Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes. Mol. Gen. Genet. 262 (2), 220-229 (1999).

Other Notes

Small volumes of anti-DBF4 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DBF4 purchase

MBS460395 is an antibody targeting DBF4. Also known as Rabbit anti DBF4 Polyclonal Antibody. Reactive in Human, Rat, Mouse. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DBF4. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
Dbf4
NCBI Official Synonym Full Names
DBF4 zinc finger L homeolog
NCBI Official Symbol
dbf4.L
NCBI Official Synonym Symbols
dbf4; dbf4a; xdbf4
UniProt Protein Name
Protein DBF4 homolog A
Protein Family
UniProt Gene Name
dbf4
UniProt Synonym Gene Names
dbf4a
NCBI Protein Information
protein DBF4 homolog A

UniProt Comments for DBF4

Regulatory subunit for cdc7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Not required during the initiation of DNA replication in egg and during early embryonic development but is required later and throughout development. The complex cdc7-dbf4a phosphorylates mcm2 subunit.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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