Rabbit Anti-DBF4B Polyclonal Antibody – Cat# MBS460402

DBF4B Antibody – Rabbit Polyclonal

Name: DBF4B Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS460402
Price: 355 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460402

The human DBF4/ASK protein contains a putative site for phosphorylation by CDK, PEST protease cleavage sites, nuclear localization signals and a short-looped zinc finger-like domain. DBF4 interacts physically with CDC7 and MCM2 as a cyclin-like regulatory… More details

$355 / 0.1 mg +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

DBF4B, Polyclonal Antibody

Full Product Name

Rabbit anti DBF4B Polyclonal Antibody

Product Gene Name

anti-DBF4B antibody

Product Synonym Names

DBF4 homolog B

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit

Species Reactivity

Human

Form/Format

Supplied in sterile Phosphate buffered saline (pH7.2) containing antibody stabilizer.

Purity/Purification

Purified by Epitope Affinity Purification

Specificity

This antibody recognizes ~77 kDa of DBF4 protein. It cross reacts to human, mouse and rat. The other species are not tested.

Preparation and Storage

The antibodies are stable for 12 months from date of receipt when stored at -20 degree C. The antibodies can be stored at 2 degree C - 8 degree C for one month without detectable loss of activity. Avoid repeated freezing-thawing cycles.

Molecular Weight

18,392 Da

OMIM

AF448801 mRNA

Preparation

A synthetic peptide corresponding to the N-terminus of DBF4B protein. This sequence is identical to human, rat and mouse.

Applications Tested/Suitable for anti-DBF4B antibody

ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB)

Application Notes for anti-DBF4B antibody

ELISA: 0.01-0.1 ug/ml
Flow Cytometry: 5-10 ug/ml
Immunohistochemistry: 2-10 ug/ml
Immunoprecipitation: 2-5 ug/ml
Western Blot: 0.1-1 ug/ml

Product References and Citations for anti-DBF4B antibody

Lepke, M., Putter, V., Staib, C., Kneissl, M., Berger, C., Hoehn, K., Nanda, I., Schmid, M. and Grummt, F. Identification, characterization and chromosomal localization of the cognate human and murine DBF4 genes. Mol. Gen. Genet. 262 (2), 220-229 (1999).

Other Notes

Small volumes of anti-DBF4B antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for DBF4B purchase

MBS460402 is an antibody targeting DBF4B. Also known as Rabbit anti DBF4B Polyclonal Antibody. Reactive in Human. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for DBF4B. It may not necessarily be applicable to this product.

NCBI GI #

16740566

NCBI GeneID

80174

NCBI Accession #

AAH16158.1

UniProt Secondary Accession #

Q8TEX0; Q96B19; Q9H912; D3DX56

UniProt Related Accession #

Q8NFT6

NCBI Official Full Name

DBF4B protein

NCBI Official Synonym Full Names

DBF4 zinc finger B

NCBI Official Symbol

DBF4B

NCBI Official Synonym Symbols

DRF1; ASKL1; CHIFB; ZDBF1B

UniProt Protein Name

Protein DBF4 homolog B

UniProt Synonym Protein Names

Activator of S phase kinase-like protein 1; ASK-like protein 1; Chiffon homolog B; Dbf4-related factor 1

Protein Family

Protein

UniProt Gene Name

DBF4B

UniProt Synonym Gene Names

ASKL1; DRF1; ASK-like protein 1

NCBI Protein Information

protein DBF4 homolog B

NCBI Summary for DBF4B

This gene encodes a regulator of the cell division cycle 7 homolog (S. cerevisiae) protein, a serine-threonine kinase which links cell cycle regulation to genome duplication. This protein localizes to the nucleus and, in complex with the cell division cycle 7 homolog (S. cerevisiae) protein, may facilitate M phase progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]

UniProt Comments for DBF4B

DBF4B: Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S and M phases. The complex CDC7-DBF4B selectively phosphorylates MCM2 subunit at 'Ser-40' and then is involved in regulating the initiation of DNA replication during cell cycle. 4 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 17q21.31|17q21

Cellular Component: cytoplasm; intracellular membrane-bound organelle; nuclear chromatin; nucleoplasm; nucleus

Molecular Function: protein binding; protein kinase activator activity; protein kinase binding

Biological Process: positive regulation of cell proliferation

Research Articles on DBF4B

1. These results indicate that Ddk functions as an upstream regulator to monitor S-phase checkpoint signaling.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.