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HBsAg Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS460736

MBS460736 is an antibody targeting HBsAg. Also known as Mouse Anti HBsAg Monoclonal Antibody. Reactive in Human. Host: Mouse. More details

$355 / 0.1 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
HBsAg, Monoclonal Antibody
Full Product Name
Mouse Anti HBsAg Monoclonal Antibody
Product Gene Name
Product Synonym Names
Mouse anti-HBsAg Monoclonal Antibody; Hepatitis B Virus, S protein; HBVgp2; Hepatitis B Surface Antigen (HBsAg); Mouse anti-HBsAg
Clonality
Monoclonal
Isotype
IgG1/M.kappa
Clone Number
ABM105
Host
Mouse
Species Reactivity
Human
Purity/Purification
Protein A affinity purification eluted according to the isotyping IgG1.kappa.
Specificity
HEPATITIS B SURFACE-ANTIGEN (HBsAg)
Affinicity Constant
2x1010 L/Mol
Immunogen
HBsAg purified from human serum by affinitive columns
Lymphocyte Strain
Babl/c mouse
Myeloma
SP2/0
Immunization Procedure
100ul of emulsion, mixed with equal volume of HBsAg solution and Freund complete adjuvant was injected by using the intracutaneous injection method to each balb/c mouse, followed by boost-inject every 2 to 3 weeks with emulsion; the mice with high titer was chosen for the fusion procedure.
Fusion Procedure
Fusion was performed by addition of chemical fusion reagent PEG to the mixture of spleen cells and myeloma cells at a ratio 5:1; Seeding the cell suspension to 96 well cell culture plates and selected according to principle of HAT drug blocking the de novo synthesis of nucleotides.
Screening Method
EIA method is used for clone screening, in which HBsAg is labeled with HRP for the screening of positive clones.
Target Antigen
HBsAg Purity>90%
Hybridoma Cloning History
The clone was screened from a total of four 96 well cell culture plate and was subcloned twice by using EIA method. Both supernatant and purified IgG/M from ascites were evaluated by the method of EIA. Further evaluation was accomplished by applying purified IgM to the EIA test against normal human sera and HBsAg positive sera specimens; no cross reaction was found with HBeAg, HBcAg, HCV and AFP.
Applications Tested/Suitable for anti-HBsAg antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Radioimmunoassay (RIA)

Product References and Citations for anti-HBsAg antibody

Lane DP and EB Lane, 1981. A rapid antibody assay system for screening hybridoma cultures, J. Immunol. Methods, 47: 303-307..

Other Notes

Small volumes of anti-HBsAg antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for HBsAg. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Official Full Name
HBsAg, partial
NCBI Official Symbol
S
NCBI Protein Information
large envelope protein;middle envelope protein;small envelope protein

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
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