Akt (Thr-34) Antibody – Rabbit Polyclonal

Akt, also known as Protein Kinase B (PKB) and Rac (Related to the A and C kinases), is a 60kDa serine/threonine kinase critical for controlling diverse cellular functions, including glucose metabolism, gene transcription, cell proliferation, and apoptosis. Akt phosphorylates a number of physiological substrates including MBP, glycogen synthetase, PKA RII subunit, and histone H1. Akt is activated in response to insulin and growth factors in a PI3-kinase dependent manner. Activation of PI3-Kinase generates phosphatidylinositol 3,4- bisphosphate which may induce the membrane translocation of Akt coincident with its phosphorylation and activation. Upon activation, Akt associates with members of the PKC family of kinases, such as PKC-delta and PKC-zeta. Ceramide-activated PKC-zeta leads to phosphorylation of the Akt-Pleckstrin Homology (PH) domain on threonine 34. This phosphorylation inhibits PIP3 binding to Akt, preventing activation of the kinase by insulin. Since Akt confers a prosurvival signal and regulates pathways in response to insulin, suppressing its activation may be one mechanism by which ceramide promotes cell death and induces insulin resistance.

• Jones, P.F. et al. (1991) Proc. Natl. Acad. Sci. USA 88:4171-4175.
• Marte, B. & Downward. J. (1997) TIBS. 22:355-358.
• Powell, D.J. et al. (2003) Mol. Cell Biol. 23:7794-7808.

Small volumes of anti-Akt (Thr-34) antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS472002 is an antibody targeting Akt (Thr-34). Also known as Akt (Thr-34), phospho-specific. Reactive in Human, Rat, Rabbit. Host: Rabbit.