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CHD3 Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475184

Entrez Summary: This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone… More details

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Product Overview

Online Datasheet in PDF
Product Name
CHD3, Monoclonal Antibody
Full Product Name
Anti-CHD3 (C-term) Mouse mAb
Product Gene Name
Product Synonym Gene Name
ZFH; Mi-2a; Mi2-ALPHA
Product Synonym Names
226; 2600010P09Rik; AF020312; ATP dependent helicase CHD3; ATP-dependent helicase Chd3; CHD-3; Chd3; CHD3_HUMAN; Chd7; Chromodomain-helicase-DNA-binding protein 3; hZFH; MGC40857; Mi 2 autoantigen 240 kDa protein; Mi 2a; Mi-2 autoantigen 240 kDa protein; Mi2 ALPHA; Mi2-alpha; Prp7; Prp9 1; ZFH; Zinc finger helicase; zinc-finger helicase (Snf2-like)
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7F6-G2-E5
Host
Mouse
Source: Human
Species Reactivity
Human, Mouse, Rat
Sensitivity
This antibody detects endogenous levels of CHD3 (C-terminus) and does not cross-react with related proteins.
Form/Format
Ascites
Molecular Weight
260
OMIM
U91543 mRNA
3D Structure
ModBase 3D Structure for Q12873
Immunogen
Purified recombinant human CHD3 (C-terminus) protein fragments expressed in E Coli.
Sequence Similarities
Belongs to the SNF2/RAD54 helicase family. Contains 2 chromo domains. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain. Contains 2 PHD-type zinc fingers
Subunit Structure
Central component of the nucleosome remodeling and histone deacetylase (NuRD) repressive complex. Interacts with TRIM28 and SERBP1. Interacts (via its C-terminal) with HABP4. Interacts with PCNT; the interaction regulates centrosome integrity.
Subcellular Location
Nucleus. Cytoplasm
Applications Tested/Suitable for anti-CHD3 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)
Application Notes for anti-CHD3 antibody
WB: 1:1000
ICC: 1:400

Western Blot (WB) of anti-CHD3 antibody

Western blot detection of CHD3 (C-terminus) in NTERA2, 3T3, C6, K562 and Hela cell lysates using CHD3 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size: 260KDa.Observed band size: 260KDa.
Mouse Anti-CHD3 Monoclonal Antibody – Cat# MBS475184

Immunocytochemistry (ICC) of anti-CHD3 antibody

Immunocytochemistry staining of HeLa cells fixed with -20 degree C Methanol and using CHD3 (C-terminus) mouse mAb (dilution 1:400).
Mouse Anti-CHD3 Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS475184

Immunoprecipitation (IP) of anti-CHD3 antibody

Immunoprecipitation analysis of Hela cell lysates using CHD3 mouse mAb.
Mouse Anti-CHD3 Monoclonal Antibody, Immunoprecipitation (IP) – Cat# MBS475184

Other Notes

Small volumes of anti-CHD3 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for CHD3 purchase

MBS475184 is an antibody targeting CHD3. Also known as Anti-CHD3 (C-term) Mouse mAb. Reactive in Human, Mouse, Rat. Host: Mouse
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for CHD3. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
chromodomain-helicase-DNA-binding protein 3 isoform 3
NCBI Official Synonym Full Names
chromodomain helicase DNA binding protein 3
NCBI Official Symbol
CHD3
NCBI Official Synonym Symbols
ZFH; Mi-2a; Mi2-ALPHA
UniProt Protein Name
Chromodomain-helicase-DNA-binding protein 3
UniProt Synonym Protein Names
ATP-dependent helicase CHD3; Mi-2 autoantigen 240 kDa protein; Mi2-alpha; Zinc finger helicase; hZFH
UniProt Gene Name
CHD3
UniProt Synonym Gene Names
CHD-3; hZFH
NCBI Protein Information
chromodomain-helicase-DNA-binding protein 3

NCBI Summary for CHD3

This gene encodes a member of the CHD family of proteins which are characterized by the presence of chromo (chromatin organization modifier) domains and SNF2-related helicase/ATPase domains. This protein is one of the components of a histone deacetylase complex referred to as the Mi-2/NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Chromatin remodeling is essential for many processes including transcription. Autoantibodies against this protein are found in a subset of patients with dermatomyositis. Three alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
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UniProt Comments for CHD3

Component of the histone deacetylase NuRD complex which participates in the remodeling of chromatin by deacetylating histones. Required for anchoring centrosomal pericentrin in both interphase and mitosis, for spindle organization and centrosome integrity.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-CHD3 antibody

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