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eIF4E Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS475628

Entrez Summary: The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of cellular mRNAs. The encoded protein aids in translation… More details

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Product Overview

Product Name
eIF4E, Monoclonal Antibody
Full Product Name
Anti-eIF4E Mouse mAb
Product Gene Name
anti-eIF4E antibody
Product Synonym Gene Name
CBP; EIF4F; AUTS19; EIF4E1; EIF4EL1
Product Synonym Names
AUTS19; CBP; CBP; eIF 4E; eIF 4F 25 kDa subunit; IF 4F 25 kDa subunit; EIF 4F; eIF-4E; eIF-4F 25 kDa subunit; eIF4E; EIF4E1; EIF4EL1; EIF4EL1; EIF4F; EIF4F; Eukaryotic Translation Initiation Factor 4 E; Eukaryotic Translation Initiation Factor 4 E; Eukaryotic translation initiation factor 4E; Eukaryotic translation initiation factor 4E like 1; IF4E_HUMAN; Messanger RNA Cap Binding Protein eIF 4E; MGC111573; MGC111573; mRNA cap binding protein; mRNA cap-binding protein
Clonality
Monoclonal
Isotype
IgG1
Clone Number
5D11-B8-H9
Host
Mouse
Source: Human
Species Reactivity
Human
Sensitivity
The antibody detects endogenous level of EIF4E protein.
Form/Format
Ascitic fluid containing 0.03% sodium azide.
Molecular Weight
25
OMIM
M15353 mRNA
3D Structure
ModBase 3D Structure for P06730
Immunogen
Purified recombinant fragment of human eIF4E expressed in E Coli.
Sequence Similarities
Belongs to the eukaryotic initiation factor 4E family.
Subunit Structure
eIF4F is a multi-subunit complex, the composition of which varies with external and internal environmental conditions. It is composed of at least EIF4A, EIF4E and EIF4G1/EIF4G3. EIF4E is also known to interact with other partners. The interaction with EIF4ENIF1 mediates the import into the nucleus. Nonphosphorylated EIF4EBP1, EIF4EBP2 and EIF4EBP3 compete with EIF4G1/EIF4G3 to interact with EIF4E; insulin stimulated MAP-kinase (MAPK1 and MAPK3) phosphorylation of EIF4EBP1 causes dissociation of the complex allowing EIF4G1/EIF4G3 to bind and consequent initiation of translation. Rapamycin can attenuate insulin stimulation, mediated by FKBPs. Interacts mutually exclusive with EIF4A1 or EIF4A2. Interacts with NGDN and PIWIL2. Component of the CYFIP1-EIF4E-FMR1 complex composed of CYFIP, EIF4E and FMR1. Interacts directly with CYFIP1. Interacts with Lassa virus Z protein. Binds to MKNK2 in nucleus. Interacts with LIMD1, WTIP and AJUBA. Interacts with APOBEC3G in an RNA-dependent manner.
Applications Tested/Suitable for anti-eIF4E antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes for anti-eIF4E antibody
WB: 1:1000
ICC: 1:100

Western Blot (WB) of anti-eIF4E antibody

Western blot detection of eIF4E in 293, K562 and Hela cell lysates using eIF4E mouse mAb (dilution 1:1000).Predicted band size:25 Kda.Observed band size:28KDa.
Mouse Anti-eIF4E Monoclonal Antibody – Cat# MBS475628

Immunocytochemistry (ICC) of anti-eIF4E antibody

Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using eIF4E mouse mAb (dilution 1:100).
Mouse Anti-eIF4E Monoclonal Antibody, Immunocytochemistry (ICC) – Cat# MBS475628

Other Notes

Small volumes of anti-eIF4E antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for eIF4E purchase

MBS475628 is an antibody targeting eIF4E. Also known as Anti-eIF4E Mouse mAb. Reactive in Human. Host: Mouse
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for eIF4E. It may not necessarily be applicable to this product.

NCBI GI #
194578907
NCBI GeneID
1977
NCBI Accession #
NP_001124150.1
UniProt Primary Accession #
P06730
UniProt Secondary Accession #
Q96E95; B7Z6V1; D6RCQ6
UniProt Related Accession #
P06730
GenBank Nucleotide #
NM_001968.3
NCBI Official Full Name
eukaryotic translation initiation factor 4E isoform 3
NCBI Official Synonym Full Names
eukaryotic translation initiation factor 4E
NCBI Official Symbol
EIF4E
NCBI Official Synonym Symbols
CBP; EIF4F; AUTS19; EIF4E1; eIF-4E; EIF4EL1
UniProt Protein Name
Eukaryotic translation initiation factor 4E
UniProt Synonym Protein Names
eIF-4F 25 kDa subunit; mRNA cap-binding protein
Protein Family
Eukaryotic translation initiation factor
UniProt Gene Name
EIF4E
UniProt Synonym Gene Names
EIF4EL1; EIF4F; eIF-4E; eIF4E
NCBI Protein Information
eukaryotic translation initiation factor 4E

NCBI Summary for eIF4E

The protein encoded by this gene is a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. Several pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
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UniProt Comments for eIF4E

Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates the binding to the mRNA cap.
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Research Articles on eIF4E

1. Our studies also suggest that nuclear entry is important for the prooncogenic activity of eIF4E, at least in this context. These findings position nuclear trafficking of eIF4E as a critical step in its regulation and position the importin 8-eIF4E complex as a novel therapeutic target.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-eIF4E antibody

 Reference Product  PubMed Publications
 EIF4EBP1 antibody  >460 publications with eIF4E and EIF4EBP1
 MKNK1 antibody  >72 publications with eIF4E and MKNK1
 EIF4B antibody  >46 publications with eIF4E and EIF4B
 EIF4EBP2 antibody  >43 publications with eIF4E and EIF4EBP2
 EIF4G1 antibody  >43 publications with eIF4E and EIF4G1
 MKNK2 antibody  >25 publications with eIF4E and MKNK2
 PML antibody  >19 publications with eIF4E and PML
 EIF4G2 antibody  >17 publications with eIF4E and EIF4G2
 EIF4ENIF1 antibody  >13 publications with eIF4E and EIF4ENIF1
 EIF4EBP3 antibody  >9 publications with eIF4E and EIF4EBP3

Pathways associated with anti-eIF4E antibody

 Products by Pathway  Pathway Diagram
 Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway antibodies  Activation Of The MRNA Upon Binding Of The Cap-binding Complex And EIFs, And Subsequent Binding To 43S Pathway Diagram
 Alpha6-Beta4 Integrin Signaling Pathway antibodies  Alpha6-Beta4 Integrin Signaling Pathway Diagram
 Antiviral Mechanism By IFN-stimulated Genes Pathway antibodies  Antiviral Mechanism By IFN-stimulated Genes Pathway Diagram
 BDNF Signaling Pathway antibodies  BDNF Signaling Pathway Diagram
 Cap-dependent Translation Initiation Pathway antibodies  Cap-dependent Translation Initiation Pathway Diagram
 Cytokine Signaling In Immune System Pathway antibodies  Cytokine Signaling In Immune System Pathway Diagram
 Deadenylation Of MRNA Pathway antibodies  Deadenylation Of MRNA Pathway Diagram
 Deadenylation-dependent MRNA Decay Pathway antibodies  Deadenylation-dependent MRNA Decay Pathway Diagram
 Eukaryotic Translation Initiation Pathway antibodies  Eukaryotic Translation Initiation Pathway Diagram
 GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway antibodies  GTP Hydrolysis And Joining Of The 60S Ribosomal Subunit Pathway Diagram

Diseases associated with anti-eIF4E antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >543 publications with eIF4E and Neoplasms
 Carcinoma Antibodies  >185 publications with eIF4E and Carcinoma
 Cell Transformation, Neoplastic Antibodies  >101 publications with eIF4E and Cell Transformation, Neoplastic
 Adenocarcinoma Antibodies  >72 publications with eIF4E and Adenocarcinoma
 Nervous System Diseases Antibodies  >64 publications with eIF4E and Nervous System Diseases
 Hypertrophy Antibodies  >52 publications with eIF4E and Hypertrophy
 Necrosis Antibodies  >48 publications with eIF4E and Necrosis
 Neoplasms, Experimental Antibodies  >46 publications with eIF4E and Neoplasms, Experimental
 Cardiovascular Diseases Antibodies  >45 publications with eIF4E and Cardiovascular Diseases
 Disease Models, Animal Antibodies  >42 publications with eIF4E and Disease Models, Animal

Organs/Tissues associated with anti-eIF4E antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >310 publications with eIF4E and Muscle
 Blood Antibodies  >250 publications with eIF4E and Blood
 Liver Antibodies  >126 publications with eIF4E and Liver
 Brain Antibodies  >116 publications with eIF4E and Brain
 Vascular Antibodies  >103 publications with eIF4E and Vascular
 Heart Antibodies  >86 publications with eIF4E and Heart
 Lung Antibodies  >75 publications with eIF4E and Lung
 Kidney Antibodies  >69 publications with eIF4E and Kidney
 Nerve Antibodies  >41 publications with eIF4E and Nerve
 Pancreas Antibodies  >38 publications with eIF4E and Pancreas
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