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c-JUN Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS476978

Entrez Summary: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by… More details

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Product Overview

Product Name
c-Jun, Polyclonal Antibody
Full Product Name
Anti-c-Jun (Phospho-Ser243) Rabbit pAb
Product Gene Name
Product Synonym Names
AH119; AP1; Jun A ; c-Jun p39
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Sensitivity
The antibody detects endogenous level of c-Jun only when phosphorylated at serine 243.
Form/Format
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1.0mg/mL (lot specific)
Molecular Weight
43
OMIM
J04111 Genomic DNA
3D Structure
ModBase 3D Structure for P05412
Immunogen
Peptide sequence around phosphorylation site of serine 243 (P-L-S (p)-P-I) derived from Human c-Jun.
Applications Tested/Suitable for anti-c-Jun antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes for anti-c-Jun antibody
WB: 1:500-1:1000
IHC: 1:50-1:100

Western Blot (WB) of anti-c-Jun antibody

Western blot analysis of extracts from sorbitol-treated 293, Serum-treated C2C12 and UV-treated HepG2 cells using c-Jun (Phospho-Ser243) Antibody.
Rabbit Anti-c-JUN Polyclonal Antibody – Cat# MBS476978

Immunohistochemistry (IHC) of anti-c-Jun antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun (Phospho-Ser243) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Rabbit Anti-c-JUN Polyclonal Antibody, Immunohistochemistry (IHC) – Cat# MBS476978

Western Blot (WB) of anti-c-Jun antibody

Western blot detection of c-Jun (Phospho-Ser243) in Hela cells untreated or serum-treated, using c-Jun (Phospho-Ser243) Rabbit pAb (dilution 1:500, upper) or beta-Actin Mouse mAb (200068-8F10, lower).Predicted band size:43kDa.Observed band size:43kDa.
Rabbit Anti-c-JUN Polyclonal Antibody, Western Blot (WB) – Cat# MBS476978

Other Notes

Small volumes of anti-c-Jun antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for c-Jun purchase

MBS476978 is an antibody targeting c-Jun. Also known as Anti-c-Jun (Phospho-Ser243) Rabbit pAb. Reactive in Human, Mouse, Rat. Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for c-Jun. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
transcription factor AP-1
NCBI Official Synonym Full Names
Jun proto-oncogene, AP-1 transcription factor subunit
NCBI Official Symbol
JUN
NCBI Official Synonym Symbols
AP1; AP-1; c-Jun
UniProt Protein Name
Transcription factor AP-1
UniProt Synonym Protein Names
Activator protein 1; AP1; Proto-oncogene c-Jun; V-jun avian sarcoma virus 17 oncogene homolog; p39
UniProt Gene Name
JUN
UniProt Synonym Gene Names
AP1
NCBI Protein Information
transcription factor AP-1

NCBI Summary for c-Jun

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies. [provided by RefSeq, Jul 2008]
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UniProt Comments for c-Jun

Transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. Promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. Involved in activated KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells (PubMed:24623306). Binds to the USP28 promoter in colorectal cancer (CRC) cells (PubMed:24623306).
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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