Rabbit Anti-BRCA1 Polyclonal Antibody – Cat# MBS477130

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using BRCA1 (Ab-1524) antibody.

BRCA1 Antibody – Rabbit Polyclonal

Name: BRCA1 Antibody; Polyclonal; Rabbit; Human-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS477130
Price: 530 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS477130

Entrez Summary: This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to… More details

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Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Product Name

BRCA1, Polyclonal Antibody

Full Product Name

Anti-BRCA1 Rabbit pAb

Product Gene Name

anti-BRCA1 antibody

Product Synonym Gene Name

IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53

Product Synonym Names

BRAC 1; BRCA 1; BRCA1; BRCA1/BRCA2 containing complex subunit 1; BRCA1/BRCA2-containing complex, subunit 1; BRCA1_HUMAN; BRCAI; BRCC 1; BRCC1; Breast and ovarian cancer susceptibility protein 1; Breast Cancer 1; Breast Cancer 1 Early Onset; Breast cancer type 1 susceptibility protein; Breast Ovarian Cancer Susceptibility; Breast Ovarian Cancer Susceptibility; BROVCA1; IRIS; Papillary Serous Carcinoma Of The Peritoneum; PNCA4; PPP1R53; Protein phosphatase 1 regulatory subunit 53; PSCP; RING finger protein 53; RING finger protein 53; RNF53

Clonality

Polyclonal

Isotype

IgG

Host

Rabbit
Source: Human

Species Reactivity

Human

Sensitivity

This antibody detects endogenous levels of BRCA1 and does not cross-react with related proteins.

Form/Format

10mM PBS with 0.02% sodium azide, 50% glycerol.

Purity/Purification

Affinity Purified

Molecular Weight

207

OMIM

U14680 mRNA

3D Structure

ModBase 3D Structure for P38398

Immunogen

The antiserum was produced against synthesized non-phosphopeptide derived from human BRCA1 around the phosphorylation site of serine 1524.

Sequence Similarities

Contains 2 BRCT domains.Contains 1 RING-type zinc finger.

Subunit Structure

Heterodimer with BARD1. Part of the BRCA1-associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the MRE11-RAD50-NBN protein (MRN) complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1, BRCC3, BRE and BABAM1. Interacts (via the BRCT domains) with FAM175A. Component of the BRCA1-RBBP8 complex. Interacts (via the BRCT domains) with RBBP8 ('Ser-327' phosphorylated form); the interaction ubiquitinates RBBP8, regulates CHEK1 activation, and involves RBBP8 in BRCA1-dependent G2/M checkpoint control on DNA damage. Associates with RNA polymerase II holoenzyme. Interacts with SMC1A and COBRA1. Interacts (via BRCT domains) with BRIP1 (phosphorylated form). Interacts with FANCD2 (ubiquitinated form). Interacts with BAP1. Interacts with DCLRE1C and CLSPN. Interacts with H2AFX (phosphorylated on 'Ser-140'). Interacts with CHEK1 and CHEK2. Interacts with BRCC3. Interacts (via the BRCT domains) with ACACA (phosphorylated form); the interaction prevents dephosphorylation of ACACA. Interacts with AURKA. Interacts with UBXN1. Part of a trimeric complex containing BRCA1, BRCA2 and PALB2. Interacts directly with PALB2; the interaction is essential for its function in HRR. Interacts directly with BRCA2; the interaction occurs only in the presence of PALB2 which serves as the bridging protein. Interacts (via the BRCT domains) with LMO4; the interaction represses the transcriptional activity of BRCA1. Interacts with KIAA0101/PAF15.

Subcellular Location

Nucleus. Chromosome. Note: Localizes at sites of DNA damage at double-strand breaks (DSBs); recruitment to DNA damage sites is mediated by the BRCA1-A complex. Isoform 3: Cytoplasm. Isoform 5: Cytoplasm.

Applications Tested/Suitable for anti-BRCA1 antibody

Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)

Other Notes

Small volumes of anti-BRCA1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for BRCA1 purchase

MBS477130 is an antibody targeting BRCA1. Also known as Anti-BRCA1 Rabbit pAb. Reactive in Human. Host: Rabbit
Source: Human.

Additional Details

NCBI/Uniprot data below describe general gene information for BRCA1. It may not necessarily be applicable to this product.

NCBI GI #

6552299

NCBI GeneID

672

NCBI Accession #

NP_009225.1

UniProt Primary Accession #

P38398

UniProt Secondary Accession #

O15129; Q1RMC1; Q3LRJ0; Q3LRJ6; Q6IN79; Q7KYU9; E9PFZ0

UniProt Related Accession #

P38398

GenBank Nucleotide #

NM_007294.3

NCBI Official Full Name

breast cancer type 1 susceptibility protein isoform 1

NCBI Official Synonym Full Names

BRCA1, DNA repair associated

NCBI Official Symbol

BRCA1

NCBI Official Synonym Symbols

IRIS; PSCP; BRCAI; BRCC1; FANCS; PNCA4; RNF53; BROVCA1; PPP1R53

UniProt Protein Name

Breast cancer type 1 susceptibility protein

UniProt Synonym Protein Names

RING finger protein 53; RING-type E3 ubiquitin transferase BRCA1Curated

Protein Family

Breast cancer type 1 susceptibility protein

UniProt Gene Name

BRCA1

UniProt Synonym Gene Names

RNF53

NCBI Protein Information

breast cancer type 1 susceptibility protein

NCBI Summary for BRCA1

This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). This gene product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. Mutations in this gene are responsible for approximately 40% of inherited breast cancers and more than 80% of inherited breast and ovarian cancers. Alternative splicing plays a role in modulating the subcellular localization and physiological function of this gene. Many alternatively spliced transcript variants, some of which are disease-associated mutations, have been described for this gene, but the full-length natures of only some of these variants has been described. A related pseudogene, which is also located on chromosome 17, has been identified. [provided by RefSeq, May 2009]

UniProt Comments for BRCA1

E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. It is unclear whether it also mediates the formation of other types of polyubiquitin chains. The E3 ubiquitin-protein ligase activity is required for its tumor suppressor function. The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Regulates centrosomal microtubule nucleation. Required for normal cell cycle progression from G2 to mitosis. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation. Contributes to homologous recombination repair (HRR) via its direct interaction with PALB2, fine-tunes recombinational repair partly through its modulatory role in the PALB2-dependent loading of BRCA2-RAD51 repair machinery at DNA breaks. Component of the BRCA1-RBBP8 complex which regulates CHEK1 activation and controls cell cycle G2/M checkpoints on DNA damage via BRCA1-mediated ubiquitination of RBBP8. Acts as a transcriptional activator (PubMed:20160719).

Research Articles on BRCA1

1. BRCA1 Mutation is associated with Breast Cancer.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-BRCA1 antibody

Reference Product	PubMed Publications
BRCA2 antibody	>4970 publications with BRCA1 and BRCA2
JUN antibody	>838 publications with BRCA1 and JUN
RAD51 antibody	>328 publications with BRCA1 and RAD51
TP53 antibody	>316 publications with BRCA1 and TP53
BARD1 antibody	>216 publications with BRCA1 and BARD1
ATR antibody	>166 publications with BRCA1 and ATR
CHEK2 antibody	>147 publications with BRCA1 and CHEK2
MLH1 antibody	>125 publications with BRCA1 and MLH1
FANCD2 antibody	>118 publications with BRCA1 and FANCD2
MYC antibody	>118 publications with BRCA1 and MYC

Pathways associated with anti-BRCA1 antibody

Products by Pathway	Pathway Diagram
ATF-2 Transcription Factor Network Pathway antibodies	ATF-2 Transcription Factor Network Pathway Diagram
ATM Mediated Phosphorylation Of Repair Proteins Pathway antibodies	ATM Mediated Phosphorylation Of Repair Proteins Pathway Diagram
ATM Mediated Response To DNA Double-strand Break Pathway antibodies	ATM Mediated Response To DNA Double-strand Break Pathway Diagram
Androgen Receptor Signaling Pathway antibodies	Androgen Receptor Signaling Pathway Diagram
Aurora A Signaling Pathway antibodies	Aurora A Signaling Pathway Diagram
BARD1 Signaling Events Pathway antibodies	BARD1 Signaling Events Pathway Diagram
BRCA1-associated Genome Surveillance Complex (BASC) Pathway antibodies	BRCA1-associated Genome Surveillance Complex (BASC) Pathway Diagram
Cell Cycle Pathway antibodies	Cell Cycle Pathway Diagram
Chromosome Maintenance Pathway antibodies	Chromosome Maintenance Pathway Diagram
Coregulation Of Androgen Receptor Activity Pathway antibodies	Coregulation Of Androgen Receptor Activity Pathway Diagram

Diseases associated with anti-BRCA1 antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>7469 publications with BRCA1 and Neoplasms
Breast Neoplasms Antibodies	>6273 publications with BRCA1 and Breast Neoplasms
Ovarian Neoplasms Antibodies	>3353 publications with BRCA1 and Ovarian Neoplasms
Carcinoma Antibodies	>1334 publications with BRCA1 and Carcinoma
Adenocarcinoma Antibodies	>572 publications with BRCA1 and Adenocarcinoma
Neoplasms, Experimental Antibodies	>357 publications with BRCA1 and Neoplasms, Experimental
Anemia Antibodies	>247 publications with BRCA1 and Anemia
Hereditary Breast and Ovarian Cancer Syndrome Antibodies	>217 publications with BRCA1 and Hereditary Breast and Ovarian Cancer Syndrome
Mammary Neoplasms, Animal Antibodies	>181 publications with BRCA1 and Mammary Neoplasms, Animal
Cell Transformation, Neoplastic Antibodies	>181 publications with BRCA1 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-BRCA1 antibody

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>572 publications with BRCA1 and Blood
Prostate Antibodies	>258 publications with BRCA1 and Prostate
Ovary Antibodies	>255 publications with BRCA1 and Ovary
Lung Antibodies	>194 publications with BRCA1 and Lung
Mammary Gland Antibodies	>175 publications with BRCA1 and Mammary Gland
Lymph Antibodies	>146 publications with BRCA1 and Lymph
Lymph Node Antibodies	>145 publications with BRCA1 and Lymph Node
Bone Antibodies	>93 publications with BRCA1 and Bone
Brain Antibodies	>84 publications with BRCA1 and Brain
Kidney Antibodies	>80 publications with BRCA1 and Kidney