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TCR Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS488504

Describes the ability desire-1 to modulate the cytolytic activity of cytotoxic T lymphocyte clone KB5-C20.Recombinant monoclonal antibody to TCR. Manufactured using our Recombinant Platform with variable regions (i.e. specificity) from the hybridoma Desire-1. More details

$635 / 0.2 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
TCR, Monoclonal Antibody
Full Product Name
Anti-TCR [Desire-1], Mouse IgG1, Kappa
Product Gene Name
Product Synonym Names
T cell receptor; T-cell receptor; Ti
Clonality
Monoclonal
Isotype
Mouse IgG1, Kappa
Clone Number
Desire-1
Host
Mouse
Species Reactivity
Mouse
Form/Format
PBS with 0.02% Proclin 300
Purity/Purification
Protein A affinity purified
Concentration
1mg/ml (lot specific)
Specificity
mAb desire-1 recognises an epitope contributed by both the alpha and beta chains of the KB5-C20 TCR.
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Molecular Weight
19,346 Da
3D Structure
ModBase 3D Structure for P01852
Animal-free Manufacturing Platform
The recombinant antibody is manufactured using a recombinant, mammalian HEK293-based expression platform. The manufacturing platform is animal-free and animal component-free.
Antibody Type
Recombinant Antibody
Immunogen
mAb desire-1 was obtained after fusion of spleen cells from a BALB.K mouse immunised twice with KB5-C20 clone cells in RPMI 1640 medium, with non-Ig-producing myeloma cells X63-Ag8.653. Desire-1 was selected for its capacity to inhibit selectively the H-2Kb-specific cytolysis by clone KB5-C20.
Chimeric Use Statement
This reformatted mouse antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.

Product Categories/Family for anti-TCR antibody

ISO Certification
Manufactured in an ISO 9001:2015 Certified Laboratory.
Applications Tested/Suitable for anti-TCR antibody
Crystallisation, Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
Application Notes for anti-TCR antibody
Desire-1 has been used for the immunoprecipitation of soluble TCR molecules (Gregoire 1991 & Hua 1986). An FITC conjugated desire-1 has been used in flow cytometric analysis as a marker for clonotype expression (Schonrich 1991). Further, a Fab fragment derived from desire-1 has been used in the crystallisation of the T-cell receptor for 3D structure determination (Housset 1997).

Immunofluorescence (IF) of anti-TCR antibody

Immunofluorescence staining of mouse splenocytes using anti-TCR antibody at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.
Mouse Anti-TCR Monoclonal Antibody – Cat# MBS488504

Product References and Citations for anti-TCR antibody

Hua et al.; Eur. J. Immunol. 1985. 15: 1029-1032

Other Notes

Small volumes of anti-TCR antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for TCR purchase

MBS488504 is an antibody targeting TCR. Also known as Anti-TCR [Desire-1], Mouse IgG1, Kappa. Reactive in Mouse. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for TCR. It may not necessarily be applicable to this product.

NCBI GI #
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
NCBI Official Full Name
T-cell receptor beta-1 chain C region
UniProt Protein Name
T-cell receptor beta-1 chain C region
Protein Family

UniProt Comments for TCR

MiscellaneousClone B10.A was isolated from a cytotoxic T lymphocyte.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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