Mouse Anti-NEFM Monoclonal Antibody – Cat# MBS500008

Neurofilament Nf-M Ms (NEFM) Antibody – Mouse Monoclonal

Name: NEFM Antibody; Monoclonal; Mouse; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS500008
Price: 435 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS500008

MBS500008 is an antibody targeting NEFM. Also known as Anti-Neurofilament M (NF-M). Reactive in Rat. Host: Mouse. Mouse monoclonal antibody More details

$435 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Neurofilament NF-M ms (NEFM), Monoclonal Antibody

Full Product Name

Anti-Neurofilament M (NF-M)

Product Gene Name

anti-NEFM antibody

Clonality

Monoclonal

Isotype

IgG1

Clone Number

3H11

Host

Mouse

Species Reactivity

Rat

Form/Format

Liquid containing 10mM sodium azide.

Purity/Purification

Total IgG fraction (Unpurified, concentrated culture supernatant)

Specificity

Specific for the ~145k neurofilament M protein.

Preparation and Storage

Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

Molecular Weight

145

Chromosome Location

Chromosome: 15; NC_005114.3 (46736787..46741632). Location: 15p12

3D Structure

ModBase 3D Structure for P12839

Antigen

Recombinant fusion protein containing the extreme C-terminus of rat NF-M expressed in and purified from E. coli.

Immunogen Information

Preparation containing the extreme C-terminus expressed in and purified from E. Coli

Immunogen Species

Rat

Reactivity Assumed Based on 100% Sequence Homology

Chicken, human, mouse

Species Reactivity Note

The antibody has been directly tested for reactivity in chicken, human, mouse and rat tissues.

Biological Significance

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2).

Applications Tested/Suitable for anti-NEFM antibody

Western Blot (WB), Immunofluorescence (IF)

Application Notes for anti-NEFM antibody

Quality Control: Western blots performed on each lot.
WB: 1:1,000
IF: 1:100

Product References and Citations for anti-NEFM antibody

• Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
• Hu YY, He SS, Wang XC, Duan QH, Khatoon S, Igbal K, Grundke-Igbal I, Wang JZ (2002) Elevated levels of phosphorylated neurofilament proteins in cerebrospinal fluid of Alzheimer disease patients. Neurosci Lett 320(3):156-60.

Other Notes

Small volumes of anti-NEFM antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEFM. It may not necessarily be applicable to this product.

NCBI GI #

8393823

NCBI GeneID

24588

NCBI Accession #

NP_058725.1

UniProt Primary Accession #

P12839

UniProt Secondary Accession #

Q63370

UniProt Related Accession #

P12839

GenBank Nucleotide #

NM_017029.2

NCBI Official Full Name

neurofilament medium polypeptide

NCBI Official Synonym Full Names

neurofilament, medium polypeptide

NCBI Official Symbol

Nefm

NCBI Official Synonym Symbols

Nfm; Nef3

UniProt Protein Name

Neurofilament medium polypeptide

UniProt Synonym Protein Names

160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein

Protein Family

Neurofilament medium polypeptide

UniProt Gene Name

Nefm

UniProt Synonym Gene Names

Nef3; Nfm; NF-M

UniProt Entry Name

NFM_RAT

NCBI Protein Information

neurofilament medium polypeptide; NF-M; neurofilament 3, medium; 160 kDa neurofilament protein; neurofilament triplet M protein; Neurofilament protein, middle polypeptide

NCBI Summary for NEFM

neuronal intermediate filament protein; important component of the cytoskeleton [RGD, Feb 2006]

UniProt Comments for NEFM

Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Post-translational modification: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Ref.4Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function.Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization

By similarity. Ref.4

Sequence similarities: Belongs to the intermediate filament family.

Research Articles on NEFM

1. head domain phosphorylation of endogenous NF-M by forskolin-activated PKA inhibits NF-M tail domain phosphorylation in rat cortical neurons

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NEFM antibody

Reference Product	PubMed Publications
Nefl antibody	>2 publications with NEFM and Nefl
Nes antibody	>1 publications with NEFM and Nes

Pathways associated with anti-NEFM antibody

Products by Pathway	Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram

Diseases associated with anti-NEFM antibody

Disease Name	Pubmed Publications
Neoplasms Antibodies	>2 publications with NEFM and Neoplasms
Nervous System Diseases Antibodies	>2 publications with NEFM and Nervous System Diseases
Peripheral Nervous System Diseases Antibodies	>1 publications with NEFM and Peripheral Nervous System Diseases
Brain Diseases Antibodies	>1 publications with NEFM and Brain Diseases

Organs/Tissues associated with anti-NEFM antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>3 publications with NEFM and Brain