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Nestin (NES) Antibody – Mouse Monoclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS500041

Target Description: Nestin is a member of the class IV intermediate filament protein family which is expressed in neuronal stem cells (Lendahl et al., 1990). Nestin was originally identified as a result of the production of a series of monoclonal antibodies… More details

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Sample Manual Insert View PDF
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Nestin, Monoclonal Antibody
Full Product Name
Anti-Nestin
Product Gene Name
anti-NES antibody
Clonality
Monoclonal
Isotype
IgG1
Clone Number
4D11
Host
Mouse
Form/Format
100ul in PBS + 50% Glycerol + 5 mM NaN3. Adequate amount of material to conduct 10 mini Western blots.
Purity/Purification
Protein G purified culture supernatant
Specificity
Specific for the ~220-240 kDa nestin doublet in Western blots of rat brain homogenate.
Preparation and Storage
Store at -20°C; stable for at least one year.
Molecular Weight
220-240 kDa
Chromosome Location
Chromosome: 1; NC_000001.11 (156668763..156677397, complement). Location: 1q23.1
OMIM
600915
3D Structure
ModBase 3D Structure for P48681
Quality Control Tests
Western blots performed on each lot.
Species Tested
Human, Mouse, Rat
Immunogen
Recombinant human Nestin expressed in and purifeid from E. coli
Applications Tested/Suitable for anti-NES antibody
Western Blot (WB), Immunofluorescence (IF)
Application Notes for anti-NES antibody
WB: 1:2,000
IF: 1:500

Western Blot (WB) of anti-NES antibody

Mouse Anti-NES Monoclonal Antibody – Cat# MBS500041

Immunostaining of anti-NES antibody

Immunostaining of cultured E20 rat cortical neurons and glia stained with anti-nestin antibody (MBS500041, red, 1:500) and anti-MAP2 antibody (MBS502140, green, 1:500). The blue is Hoechst staining for nuclear DNA. The nestin antibody labels developing astrocytes and neuronal stem cells in a clearly filamentous fashion, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
Mouse Anti-NES Monoclonal Antibody, Immunostaining – Cat# MBS500041

Product References and Citations for anti-NES antibody

• Lendahl U, Zimmerman LB and McKay RD. CNS stem cells express a new class of intermediate filament protein. Cell 60:585-95 (1990).
• Hockfield S and McKay RD. Identification of major cell classes in the developing mammalian nervous system. J. Neurosci. 5:3310-3328 (1985).

Other Notes

Small volumes of anti-NES antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for NES purchase

MBS500041 is an antibody targeting NES. Also known as Anti-Nestin. Host: Mouse.

Additional Details

NCBI/Uniprot data below describe general gene information for NES. It may not necessarily be applicable to this product.

NCBI GI #
38176300
NCBI GeneID
10763
NCBI Accession #
NP_006608.1
UniProt Primary Accession #
P48681
UniProt Secondary Accession #
O00552; Q3LIF5; Q5SYZ6
UniProt Related Accession #
P48681
GenBank Nucleotide #
NM_006617.1
NCBI Official Full Name
nestin
NCBI Official Synonym Full Names
nestin
NCBI Official Symbol
NES
NCBI Official Synonym Symbols
Nbla00170
UniProt Protein Name
Nestin
Protein Family
Nestin
UniProt Gene Name
NES
UniProt Entry Name
NEST_HUMAN
NCBI Protein Information
nestin

NCBI Summary for NES

This gene encodes a member of the intermediate filament protein family and is expressed primarily in nerve cells. [provided by RefSeq, Sep 2011]

UniProt Comments for NES

Function: Required for brain and eye development. Promotes the disassembly of phosphorylated vimentin intermediate filaments (IF) during mitosis and may play a role in the trafficking and distribution of IF proteins and other cellular factors to daughter cells during progenitor cell division. Required for survival, renewal and mitogen-stimulated proliferation of neural progenitor cells

By similarity.

Subunit structure: Forms homodimers and homotetramers in vitro. In mixtures with other intermediate filament proteins such as vimentin and alpha-internexin, tis protein preferentially forms heterodimers which can assemble to form intermediate filaments if nestin does not exceed 25%. Interacts with FHOD3

By similarity.

Tissue specificity: CNS stem cells.

Developmental stage: Upon terminal neural differentiation, nestin is down-regulated and replaced by neurofilaments.

Post-translational modification: Constitutively phosphorylated. This increases during mitosis when the cytoplasmic intermediate filament network is reorganized

By similarity.

Sequence similarities: Belongs to the intermediate filament family.
Show more

Research Articles on NES

1. Data indicate that the only constantly present diagnostic marker observed was S100 protein and, in lesser extent, also nestin.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-NES antibody

 Reference Product  PubMed Publications
 EGF antibody  >69 publications with NES and EGF
 BMP4 antibody  >15 publications with NES and BMP4
 MYCN antibody  >14 publications with NES and MYCN
 SOX10 antibody  >13 publications with NES and SOX10
 CDK5 antibody  >12 publications with NES and CDK5
 VSX2 antibody  >6 publications with NES and VSX2

Diseases associated with anti-NES antibody

 Disease Name  Pubmed Publications
 Nervous System Diseases Antibodies  >913 publications with NES and Nervous System Diseases
 Brain Diseases Antibodies  >648 publications with NES and Brain Diseases
 Neoplasms Antibodies  >613 publications with NES and Neoplasms
 Disease Models, Animal Antibodies  >240 publications with NES and Disease Models, Animal
 Cardiovascular Diseases Antibodies  >202 publications with NES and Cardiovascular Diseases
 Seizures Antibodies  >144 publications with NES and Seizures
 Nerve Degeneration Antibodies  >73 publications with NES and Nerve Degeneration
 Breast Neoplasms Antibodies  >67 publications with NES and Breast Neoplasms
 Adenocarcinoma Antibodies  >64 publications with NES and Adenocarcinoma
 Neoplasms, Experimental Antibodies  >55 publications with NES and Neoplasms, Experimental

Organs/Tissues associated with anti-NES antibody

 Organ/Tissue Name  Pubmed Publications
 Nerve Antibodies  >2063 publications with NES and Nerve
 Brain Antibodies  >1252 publications with NES and Brain
 Embryonic Tissue Antibodies  >695 publications with NES and Embryonic Tissue
 Muscle Antibodies  >243 publications with NES and Muscle
 Vascular Antibodies  >231 publications with NES and Vascular
 Eye Antibodies  >166 publications with NES and Eye
 Skin Antibodies  >135 publications with NES and Skin
 Pancreas Antibodies  >114 publications with NES and Pancreas
 Heart Antibodies  >95 publications with NES and Heart
 Kidney Antibodies  >95 publications with NES and Kidney
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