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Fibroblast Growth Factor, Basic Active Protein

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS650175

Fibroblast Growth Factor-basic (FGF-b or FGF-2) is a heparin binding growth factor which stimulates the proliferation of a wide variety of cells including mesenchymal, neuroectodermal and endothelial cells. FGF-b also exerts a potent angiogenic activity in… More details

$370 / 0.01 mg +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Certificate of Analysis (COA) View PDF
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QC Report View PDF
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Material Safety Data Sheet View PDF
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Technical Datasheet View PDF
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Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Fibroblast Growth Factor, Basic, Active Protein
Full Product Name
Fibroblast Growth Factor, Basic, Recombinant, Human (FGF2, BFGF, FGFB, HBGF-2, HBGH-2, Heparin-binding growth factor 2 precursor, Prostatropin)
Sequence
The first five N-terminal aa: Met-Ala-Al a-Gly-Ser. This correspond s to native human FGF-b: MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Host
Recombinant Human (E Coli)
Form/Format
Supplied as a liqlyophilized powder from 5mM TBS, pH 7.5. Reconstitution: Reconstitute with sterile, ddH2O, 0.1% HSA or BSA to.01mg/ml.
Purity/Purification
Highly Purified
98% by SDS-PAGE and HPLC analyses. Contaminants
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer
Molecular Weight
17.2kD
Active Protein Note
Protein activity can be an ambiguous term. Please refer to the data sheet or inquire with Technical Support to determine the definition of activity for this product.
Biological Activity
Human FGF-b is fully biologically active when compared to standards. The ED50 as determined by the dose-dependent stimulation of thymidine uptake by BaF3 cells expressing FGF receptors is  0.5ng/ml, corresponding to a specific activity of  2x10e6u/mg.
Contaminants
Dimers and aggregates. 2%

Product Categories/Family for Fibroblast Growth Factor, Basic active protein

Growth Factors; Cytokines; Growth Factors-FGF

Other Notes

Small volumes of Fibroblast Growth Factor, Basic active protein vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for Fibroblast Growth Factor, Basic purchase

MBS650175 is an active protein targeting Fibroblast Growth Factor, Basic. Also known as Fibroblast Growth Factor, Basic, Recombinant, Human (FGF2, BFGF, FGFB, HBGF-2, HBGH-2, Heparin-binding growth factor 2 precursor, Prostatropin). Host: Recombinant Human (E Coli).
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