• Call +1.858.633.0165 or Fax +1.858.633.0166 or Contact Us

Dopamine Beta-Hydroxylase, C-Terminus, Human (DBH) Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502023

MBS502023 is an antibody targeting DBH. Also known as Anti-Dopamine beta-Hydroxylase, C-Terminus. Reactive in Human, mouse, and non-human primate. Host: Sheep. Affinity purified sheep polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB
Product Documentation
PDF
Sample Manual Insert View PDF
PDF
Testing Data View PDF
PDF
Certificate of Analysis (COA) View PDF
PDF
QC Report View PDF
PDF
Material Safety Data Sheet View PDF
PDF
Technical Datasheet View PDF
PDF
Protocol View PDF

Product Overview

Online Datasheet in PDF
Product Name
Dopamine beta-Hydroxylase, C-Terminus, Human (DBH), Polyclonal Antibody
Full Product Name
Anti-Dopamine beta-Hydroxylase, C-Terminus
Product Gene Name
anti-DBH antibody
Clonality
Polyclonal
Host
Sheep
Species Reactivity
Human, mouse, and non-human primate
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Purity/Purification
Affinity Purified (Prepared from sheep serum by affinity purification using a Sulfo-Link column matrix to which the peptide immunogen was coupled.)
Specificity
Specific for the ~75k DBH protein in Western blots.
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Molecular Weight
75
Chromosome Location
Chromosome: 9; NC_000009.12 (133636363..133659344). Location: 9q34
OMIM
223360
3D Structure
ModBase 3D Structure for P09172
Antigen
Peptide from the C-terminal region of human dopamine beta-hydroxylase (DBH), conjugated to keyhole limpet hemocyanin (KLH).
Immunogen Information
Synthetic peptide corresponding to amino acid residues from the C-terminal region conjugated to KLH
Immunogen Species
Human
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with human, mouse, and non-human primate tissue.
Biological Significance
DBH catalyzes the conversion of dopamine to norepinephrine and serves as a marker of noradrenergic cells. DBH antibodies and antibodies for other markers of catecholamine biosynthesis are widely used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). The expression of DBH is also elevated during stress (Sabban and Kvetnansky, 2001).
Applications Tested/Suitable for anti-DBH antibody
Western Blot (WB)
Application Notes for anti-DBH antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000

Testing Data of anti-DBH antibody

Sheep Anti-DBH Polyclonal Antibody – Cat# MBS502023

Product References and Citations for anti-DBH antibody

• Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.
• Sabban EL, Kvetnansky R (2001) Stress-triggered activation of gene expression in catecholaminergic systems: dynamics of transcriptional events. Trends Neurosci 24:91-98.
• Zhu MY, Klimek V, Haycock JW, Ordway GA (2000) Quantitation of tyrosine hydroxylase protein in the locus coeruleus from postmortem human brain. J Neurosci Meth 99:37-44.
• Zhu MY, Klimek V, Dilley GE, Haycock JW, Stockmeier C, Overholser JC, Meltzer HY, Ordway GA (1999) Elevated levels of tyrosine hydroxylase in the locus coeruleus in major depression. Biol Psychiatry 46:1275-1286.

Other Notes

Small volumes of anti-DBH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for DBH. It may not necessarily be applicable to this product.

NCBI GI #
116534900
NCBI GeneID
1621
NCBI Accession #
NP_000778.3
UniProt Primary Accession #
P09172
UniProt Secondary Accession #
Q5T381; Q96AG2
UniProt Related Accession #
P09172
GenBank Nucleotide #
NM_000787.3
NCBI Official Full Name
dopamine beta-hydroxylase
NCBI Official Synonym Full Names
dopamine beta-hydroxylase (dopamine beta-monooxygenase)
NCBI Official Symbol
DBH
NCBI Official Synonym Symbols
DBM
UniProt Protein Name
Dopamine beta-hydroxylase
UniProt Synonym Protein Names
Dopamine beta-monooxygenase
Protein Family
Dopamine beta-hydroxylase
UniProt Gene Name
DBH
UniProt Entry Name
DOPO_HUMAN
NCBI Protein Information
dopamine beta-hydroxylase

NCBI Summary for DBH

The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008]
Show more

UniProt Comments for DBH

Function: Conversion of dopamine to noradrenaline.

Catalytic activity: 3,4-dihydroxyphenethylamine + ascorbate + O2 = noradrenaline + dehydroascorbate + H2O.

Cofactor: Binds 1 PQQ per subunit.Binds 2 copper ions per subunit.

Pathway: Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1.

Subunit structure: Homotetramer composed of two non-covalently bound disulfide-linked dimers.

Subcellular location: Soluble dopamine beta-hydroxylase: Cytoplasmic vesicle › secretory vesicle lumen. Cytoplasmic vesicle › secretory vesicle › chromaffin granule lumen. Cytoplasmic vesicle › secretory vesicle membrane; Single-pass type II membrane protein. Cytoplasmic vesicle › secretory vesicle › chromaffin granule membrane; Single-pass type II membrane protein

Potential.

Induction: Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids.

Polymorphism: There are two main alleles of DBH: DBH-A with Ala-318 and DBH-B with Ser-318.

Involvement in disease: Dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]: Characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.Note: The disease is caused by mutations affecting the gene represented in this entry.

Sequence similarities: Belongs to the copper type II ascorbate-dependent monooxygenase family.Contains 1 DOMON domain.

Sequence caution: The sequence AAH17174.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA31631.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA31632.1 differs from that shown. Reason: Erroneous initiation. The sequence CAA68285.1 differs from that shown. Reason: Erroneous initiation.
Show more

Research Articles on DBH

1. These results suggested that dopamine beta hydroxylase gene (DBH) might be related to attention-deficit hyperactivity disorder (ADHD)symptoms in korean children.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-DBH antibody

 Reference Product  PubMed Publications
 PNMT antibody  >186 publications with DBH and PNMT
 NPY antibody  >117 publications with DBH and NPY
 COMT antibody  >70 publications with DBH and COMT
 DDC antibody  >32 publications with DBH and DDC
 MAOA antibody  >24 publications with DBH and MAOA
 MAOB antibody  >3 publications with DBH and MAOB
 CYB561 antibody  >1 publications with DBH and CYB561

Pathways associated with anti-DBH antibody

 Products by Pathway  Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Catecholamine Biosynthesis Pathway antibodies  Catecholamine Biosynthesis Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Metabolism Pathway antibodies  Metabolism Pathway Diagram
 Metabolism Of Amino Acids And Derivatives Pathway antibodies  Metabolism Of Amino Acids And Derivatives Pathway Diagram
 Monoamine Transport Pathway antibodies  Monoamine Transport Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram
 Tyrosine Metabolism Pathway antibodies  Tyrosine Metabolism Pathway Diagram

Diseases associated with anti-DBH antibody

 Disease Name  Pubmed Publications
 Mental Disorders Antibodies  >236 publications with DBH and Mental Disorders
 Nervous System Diseases Antibodies  >206 publications with DBH and Nervous System Diseases
 Hypertension Antibodies  >92 publications with DBH and Hypertension
 Movement Disorders Antibodies  >54 publications with DBH and Movement Disorders
 Mood Disorders Antibodies  >50 publications with DBH and Mood Disorders
 Hypotension Antibodies  >41 publications with DBH and Hypotension
 Basal Ganglia Diseases Antibodies  >40 publications with DBH and Basal Ganglia Diseases
 Depressive Disorder Antibodies  >35 publications with DBH and Depressive Disorder
 Bipolar Disorder Antibodies  >27 publications with DBH and Bipolar Disorder
 Parkinsonian Disorders Antibodies  >25 publications with DBH and Parkinsonian Disorders

Organs/Tissues associated with anti-DBH antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >596 publications with DBH and Brain
 Nerve Antibodies  >377 publications with DBH and Nerve
 Adrenal Gland Antibodies  >216 publications with DBH and Adrenal Gland
 Ganglia Antibodies  >157 publications with DBH and Ganglia
 Embryonic Tissue Antibodies  >29 publications with DBH and Embryonic Tissue
 Mouth Antibodies  >16 publications with DBH and Mouth
 Testis Antibodies  >7 publications with DBH and Testis
 Lymph Node Antibodies  >5 publications with DBH and Lymph Node
Request a Quote

Please fill out the form below and our representative will get back to you shortly.

MBS000000
Contact Us

Please fill out the form below and our representative will get back to you shortly.

MBS000000