GABAA Receptor a 6 (GABRA6) Antibody – Rabbit Polyclonal

Polyclonal

Rabbit

Rat, mouse

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Affinity Purified (Prepared from rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.)

Specific for the ~57k alpha6-subunit of the GABAA receptor in Western blots. Labeling is absent in alpha6-subunit knockout animals.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

57

Chromosome: 10; NC_005109.3 (27691630..27706971, complement). Location: 10q21

ModBase 3D Structure for P30191

Fusion protein from the cytoplasmic loop of the alpha6-subunit of rat GABAA receptor.

Fusion protein from the cytoplasmic loop of the alpha 6 subunit

Rat

The antibody has been directly tested for reactivity in Western blots with rat and mouse tissue.

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, coexpression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003).