GABAA Receptor G2 (GABRG2) Antibody – Rabbit Polyclonal

Polyclonal

Rabbit

Rat

50 ul neat serum. Adequate material to conduct 10 mini-Western blots.

Neat Serum (Unpurified neat serum.)

Specific for the ~46k gamma2-subunit of the GABAA receptor in Western blots of rat brain extracts.

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

46

Chromosome: 10; NC_005109.3 (26934991..27024440, complement). Location: 10q21

ModBase 3D Structure for P18508

Peptide representing a sequence that is specific for the gamma2-subunit of rat GABAA receptor. The sequence of the immunogen has no homology with any other GABAA R-subunit.

Synthetic peptide corresponding to amino acid residues specific to the gamma 2 subunit conjugated to KLH

Rat

Chicken, human, mouse, non-human primates and zebra fish

The antibody has been directly tested for reactivity in rat tissue. It is anticipated that the antibody will also work with bovine, chicken, human, mouse, non-human primate and zebra fish tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl- channel associated with the GABAA receptor (GABAA-R) subtype. GABAA-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABAA-R is a multimeric subunit complex. To date six alphas, four betas and four gammas, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for alpha- and beta-subunits results in the expression of functional GABAA-Rs sensitive to GABA. However, co-expression of a gamma-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different alpha-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). Lastly, phosphorylation of beta-subunits of the receptor has been shown to modulate GABAA-R function (Brandon et al., 2003).