Rabbit Anti-SYT1 Polyclonal Antibody – Cat# MBS502122

Synaptotagmin (Ser309) (SYT1) Antibody – Rabbit Polyclonal

Name: SYT1 Antibody; Polyclonal; Rabbit; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502122
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502122

MBS502122 is an antibody targeting SYT1. Also known as Anti-Phospho-Ser309 Synaptotagmin. Reactive in Rat. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Synaptotagmin (Ser309) (SYT1), Polyclonal Antibody

Full Product Name

Anti-Phospho-Ser309 Synaptotagmin

Product Gene Name

anti-SYT1 antibody

Clonality

Polyclonal

Host

Rabbit

Species Reactivity

Rat

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.

Purity/Purification

Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Specificity

Specific for the ~60k-62k synaptotagmin protein phosphorylated at Ser309.

Preparation and Storage

For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.

Molecular Weight

60/62

Chromosome Location

Chromosome: 7; NC_005106.3 (50097034..50289950, complement). Location: 7q21

3D Structure

ModBase 3D Structure for P21707

Antigen

Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser309 of synaptotagmin.

Immunogen Information

Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser309 conjugated to KLH

Immunogen Species

Rat

Reactivity Assumed Based on 100% Sequence Homology

Bovine, canine, chicken, human, mouse, non-human primate and zebra fish

Species Reactivity Note

The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and zebra fish based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Biological Significance

Synaptotagmin is widely regarded as the primary calcium sensor for synaptic vesicle exocytosis (Fernandez-Chacon et al., 2001; Wang et al., 2003). Moreover, recent studies indicate that the protein also plays a key role in endocytosis (Poskanzer et al., 2003). Synaptotagmin can be phosphorylated by multiple protein kinases and this may play a key role in modulation of synaptotagmin's ability to influence both the exocytotic and endocytotic components of synaptic transmission (Hilfiker et al., 1999; Lee et al., 2004).

Applications Tested/Suitable for anti-SYT1 antibody

Western Blot (WB), Immunohistochemistry (IHC)

Application Notes for anti-SYT1 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC: 1:400

Product References and Citations for anti-SYT1 antibody

• Fernandez-Chacon R, Konigstorfer A, Gerber SH, Garcia J, Matos MF, Stevens CF, Brose N, Rizo J, Rosenmund C, Sudhof TC (2001) Synaptotagmin I functions as a calcium regulator of release probability. Nature (London) 410:41-49.
• Hilfiker S, Pieribone VA, Nordstedt C, Greengard P, Czernik AJ (1999) Regulation of synaptotagmin I phosphorylation by multiple protein kinases. J Neurochem 73:921-932.
• Lee BH, Min X, Heise CJ, Xu BE, Chen S, Shu H, Luby-Phelps K, Goldsmith EJ, Cobb MH (2004) WNK1 phosphorylates synaptotagmin 2 and modulates its membrane binding. Mol Cell 15:741-751.
• Poskanzer KE, Marek KW, Sweeney ST, Davis GW (2003) Synaptotagmin I is necessary for compensatory synaptic vesicle endocytosis in vivo. Nature (London) 426:559-563.
• Wang CT, Lu JC, Bai JH, Chang PY, Martin TFJ, Chapman ER, Jackson MB (2003) Different domains of synaptotagmin control the choice between kiss-and-run and full fusion. Nature (London) 424:943-947.

Other Notes

Small volumes of anti-SYT1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for SYT1. It may not necessarily be applicable to this product.

NCBI GI #

148356226

NCBI GeneID

25716

NCBI Accession #

NP_001028852.2

UniProt Primary Accession #

P21707

UniProt Secondary Accession #

Q3S2E6; Q707P5

UniProt Related Accession #

P21707

GenBank Nucleotide #

NM_001033680.2

NCBI Official Full Name

synaptotagmin-1

NCBI Official Synonym Full Names

synaptotagmin I

NCBI Official Symbol

Syt1

NCBI Official Synonym Symbols

P65

UniProt Protein Name

Synaptotagmin-1

UniProt Synonym Protein Names

Synaptotagmin I; SytI; p65

Protein Family

ARF guanine-nucleotide exchange factor

UniProt Gene Name

Syt1

UniProt Synonym Gene Names

SytI

UniProt Entry Name

SYT1_RAT

NCBI Protein Information

synaptotagmin-1; sytI; synaptotagmin 1

NCBI Summary for SYT1

integral component of synaptic vesicle membranes; important for exocytosis and vesicle trafficking to the active zone of synapses; may be the calcium sensor for calcium-dependent exocytosis [RGD, Feb 2006]

UniProt Comments for SYT1

Function: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner; these are neurexins, syntaxin and AP2.

Cofactor: Binds 3 calcium ions per subunit. The ions are bound to the C2 domains.

Subunit structure: Homotetramer

Probable. Interacts with SCAMP5 and STON2. Forms a complex with SV2B, syntaxin 1 and SNAP25

By similarity. Interacts with RIMS1. Interacts with SV2A, SV2B and SV2C. Ref.5 Ref.6 Ref.8

Subcellular location: Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Single-pass membrane protein. Cytoplasmic vesicle › secretory vesicle › chromaffin granule membrane; Single-pass membrane protein. Cytoplasm. Note: Synaptic vesicles and chromaffin granules.

Tissue specificity: Predominantly expressed in rostral, phylogenetically younger brain regions, and in some endocrine tissues.

Domain: The first C2 domain mediates Ca2+-dependent phospholipid binding. Ref.5The second C2 domain mediates interaction with SV2A and probably with STN2. Ref.5

Sequence similarities: Belongs to the synaptotagmin family.Contains 2 C2 domains.

Research Articles on SYT1

1. Analysis of SNARE complex/synaptotagmin-1 interactions by one-dimensional NMR Spectroscopy

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-SYT1 antibody

Products by Pathway	Pathway Diagram
Acetylcholine Neurotransmitter Release Cycle Pathway antibodies	Acetylcholine Neurotransmitter Release Cycle Pathway Diagram
GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies	GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram
Glutamate Neurotransmitter Release Cycle Pathway antibodies	Glutamate Neurotransmitter Release Cycle Pathway Diagram
Neuronal System Pathway antibodies	Neuronal System Pathway Diagram
Neurotransmitter Release Cycle Pathway antibodies	Neurotransmitter Release Cycle Pathway Diagram
Norepinephrine Neurotransmitter Release Cycle Pathway antibodies	Norepinephrine Neurotransmitter Release Cycle Pathway Diagram
Synaptic Vesicle Cycle Pathway antibodies	Synaptic Vesicle Cycle Pathway Diagram
Synaptic Vesicle Cycle Pathway antibodies	Synaptic Vesicle Cycle Pathway Diagram
Transmission Across Chemical Synapses Pathway antibodies	Transmission Across Chemical Synapses Pathway Diagram

Diseases associated with anti-SYT1 antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>25 publications with SYT1 and Nervous System Diseases
Brain Diseases Antibodies	>16 publications with SYT1 and Brain Diseases
Neoplasms Antibodies	>12 publications with SYT1 and Neoplasms
Disease Models, Animal Antibodies	>7 publications with SYT1 and Disease Models, Animal
Inflammation Antibodies	>4 publications with SYT1 and Inflammation
Seizures Antibodies	>4 publications with SYT1 and Seizures
Movement Disorders Antibodies	>3 publications with SYT1 and Movement Disorders
Learning Disorders Antibodies	>3 publications with SYT1 and Learning Disorders
Poisoning Antibodies	>2 publications with SYT1 and Poisoning
Pancreatic Neoplasms Antibodies	>2 publications with SYT1 and Pancreatic Neoplasms

Organs/Tissues associated with anti-SYT1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>123 publications with SYT1 and Brain
Eye Antibodies	>4 publications with SYT1 and Eye
Dorsal Root Ganglion Antibodies	>3 publications with SYT1 and Dorsal Root Ganglion
Pineal Gland Antibodies	>2 publications with SYT1 and Pineal Gland