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Tyrosine Hydroxylase (Ser31) (TH) Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502133

MBS502133 is an antibody targeting TH. Also known as Anti-Phospho-Ser31 Tyrosine Hydroxylase. Reactive in Rat, mouse. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB
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Testing Data View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Tyrosine Hydroxylase (Ser31) (TH), Polyclonal Antibody
Full Product Name
Anti-Phospho-Ser31 Tyrosine Hydroxylase
Product Gene Name
anti-TH antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat, mouse
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots.
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Specificity
Specific for the ~60k tyrosine hydroxylase protein phosphorylated at Ser31.
Preparation and Storage
For long term storage -20 degree C is recommended. Stable at -20 degree C for at least 1 year.
Molecular Weight
60
Chromosome Location
Chromosome: 1; NC_005100.3 (222935462..222942715, complement). Location: 1q41
3D Structure
ModBase 3D Structure for P04177
Antigen
Phosphopeptide corresponding to amino acid residues surrounding phospho-Ser31 of rat tyrosine hydroxylase (TH).
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser31 conjugated to KLH
Immunogen Species
Rat
Reactivity Assumed Based on 100% Sequence Homology
Non-human primates
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots in rat and mouse tissues. It is anticipated that the antibody will also work with non-human primate tissues based on the fact that this species has 100% homology with the amino acid sequence used as antigen.
Biological Significance
Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson's disease and drug abuse (Kish et al., 2001; Zhu et al., 2000; Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000; Salvatore et al., 2001; Dunkley et al., 2004). The activity of TH is also regulated by phosphorylation (Haycock et al., 1982; Haycock et al., 1992; Jedynak et al., 2002). Phospho-specific antibodies for the phosphorylation sites on TH can be used to great effect in studying this regulation and in identifying the cells in which TH phosphorylation occurs.
Applications Tested/Suitable for anti-TH antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-TH antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000
IHC (frozen sections; Witkovsky et al., 2000): 1:1000
IF (frozen sections; Witkovsky et al., 2000): 1:1000

Testing Data of anti-TH antibody

Rabbit Anti-TH Polyclonal Antibody – Cat# MBS502133

Product References and Citations for anti-TH antibody

• Dunkley PR, Bobrovskaya L, Graham ME, Von Nagy-Felsobuki EI, Dickson PW (2004) Tyrosine hydroxylase phosphorylation: regulation and consequences. J Neurochem 91:1025-1043.
• Haycock JW, Ahn NG, Cobb MH, Krebs EG (1992) ERK1 and ERK2, two microtubule-associated protein 2 kinases, mediate the phosphorylation of tyrosine hydroxylase at serine-31 in situ. Proc Natl Acad Sci (USA) 89:2365-2369.
• Haycock JW, Bennett WF, George RJ, Waymire JC (1982) Multiple site phosphorylation of tyrosine hydroxylase. Differential regulation in situ by a 8-bromo-cAMP and acetylcholine. J Biol Chem 257:13699-13703
• Jedynak JP, Ali SF, Haycock JW, Hope BT (2002) Acute administration of cocaine regulates the phosphorylation of serine-19,-31 and-40 in tyrosine hydroxylase. J Neurochem 82:382-388.
• Kish SJ, Kalasinsky KS, Derkach P, Schmunk GA, Guttman M, Ang L, Adams V, Furukawa Y, Haycock JW (2001) Striatal dopaminergic and serotonergic markers in human heroin users. Neuropsychopharmacology 24:561-567.

Other Notes

Small volumes of anti-TH antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for TH. It may not necessarily be applicable to this product.

NCBI GI #
6981652
NCBI GeneID
25085
NCBI Accession #
NP_036872.1
UniProt Primary Accession #
P04177
UniProt Related Accession #
P04177
GenBank Nucleotide #
NM_012740.3
NCBI Official Full Name
tyrosine 3-monooxygenase
NCBI Official Synonym Full Names
tyrosine hydroxylase
NCBI Official Symbol
Th
NCBI Official Synonym Symbols
The
UniProt Protein Name
Tyrosine 3-monooxygenase
UniProt Synonym Protein Names
Tyrosine 3-hydroxylase
UniProt Gene Name
Th
UniProt Synonym Gene Names
TH
UniProt Entry Name
TY3H_RAT
NCBI Protein Information
tyrosine 3-monooxygenase; tyrosine 3-hydroxylase

NCBI Summary for TH

catalyzes the rate limiting step in catecholamine biosynthesis; may be involved in response to hypoxia [RGD, Feb 2006]

UniProt Comments for TH

Function: Plays an important role in the physiology of adrenergic neurons.

Catalytic activity: L-tyrosine + tetrahydrobiopterin + O2 = L-dopa + 4a-hydroxytetrahydrobiopterin.

Cofactor: Fe2+ ion.

Enzyme regulation: Phosphorylation leads to an increase in the catalytic activity.

Pathway: Catecholamine biosynthesis; dopamine biosynthesis; dopamine from L-tyrosine: step 1/2.

Subunit structure: Homotetramer.

Post-translational modification: In vitro, phosphorylation of Ser-19 increases the rate of Ser-40 phosphorylation, which results in enzyme opening and activation.

Sequence similarities: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
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Research Articles on TH

1. Disruption of the blood-brain barrier results in increased activation of Th-containing cells by circulating angiotensin II.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-TH antibody

 Products by Pathway  Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Alcoholism Pathway antibodies  Alcoholism Pathway Diagram
 Amine-derived Hormones Pathway antibodies  Amine-derived Hormones Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Amphetamine Addiction Pathway antibodies  Amphetamine Addiction Pathway Diagram
 Biogenic Amine Synthesis Pathway antibodies  Biogenic Amine Synthesis Pathway Diagram
 Catecholamine Biosynthesis Pathway antibodies  Catecholamine Biosynthesis Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway antibodies  Catecholamine Biosynthesis, Tyrosine => Dopamine => Noradrenaline => Adrenaline Pathway Diagram
 Catecholamine Synthesis Pathway antibodies  Catecholamine Synthesis Pathway Diagram

Diseases associated with anti-TH antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >1195651 publications with TH and Neoplasms
 Nervous System Diseases Antibodies  >871575 publications with TH and Nervous System Diseases
 Heart Diseases Antibodies  >480111 publications with TH and Heart Diseases
 Brain Diseases Antibodies  >416752 publications with TH and Brain Diseases
 Pain Antibodies  >319480 publications with TH and Pain
 Kidney Diseases Antibodies  >208533 publications with TH and Kidney Diseases
 Inflammation Antibodies  >172144 publications with TH and Inflammation
 Hypertension Antibodies  >172135 publications with TH and Hypertension
 Necrosis Antibodies  >111201 publications with TH and Necrosis
 Myocardial Infarction Antibodies  >104291 publications with TH and Myocardial Infarction

Organs/Tissues associated with anti-TH antibody

 Organ/Tissue Name  Pubmed Publications
 Brain Antibodies  >281608 publications with TH and Brain
 Prostate Antibodies  >61828 publications with TH and Prostate
 Adrenal Gland Antibodies  >19434 publications with TH and Adrenal Gland
 Uncharacterized Tissue Antibodies  >100 publications with TH and Uncharacterized Tissue
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