Chicken Anti-NEFL Polyclonal Antibody – Cat# MBS502142

Neurofilament Nf-L Ck (NEFL) Antibody – Chicken Polyclonal

Name: NEFL Antibody; Polyclonal; Chicken; Rat-Reactive; MyBioSource
Brand: MyBioSource
SKU: MBS502142
Price: 435 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502142

MBS502142 is an antibody targeting NEFL. Also known as Anti-Neurofilament L (NF-L). Reactive in Rat. Host: Chicken. Chicken polyclonal antibody More details

$435 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

Neurofilament NF-L ck (NEFL), Polyclonal Antibody

Full Product Name

Anti-Neurofilament L (NF-L)

Product Gene Name

anti-NEFL antibody

Clonality

Polyclonal

Host

Chicken

Species Reactivity

Rat

Form/Format

Liquid containing 10mM sodium azide.

Purity/Purification

Total IgY fraction

Specificity

Specific for the ~68k Neurofilament L protein in Western blots and works well on frozen sections, cells in tissue culture and on mildly formalin fixed histological sections.

Preparation and Storage

Store at -20 degree C in undiluted aliquots; stable for at least 1 year. Avoid freeze/thaw cycles.

Molecular Weight

Chromosome Location

Chromosome: 8; AC_000165.1 (73178729..73184425, complement). Location: chromosome: 8

3D Structure

ModBase 3D Structure for P02548

Antigen

Preparation of bovine spinal cord intermediate filaments

Immunogen Information

Preparation of bovine spinal cord NF-L

Immunogen Species

Bovine

Reactivity Assumed Based on 100% Sequence Homology

Chicken, human, mouse

Species Reactivity Note

The antibody has been directly tested for reactivity in bovine, chicken, human, mouse and rat tissues.

Biological Significance

Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-L is the neurofilament light or low molecular weight polypeptide and runs on SDS-PAGE gels at about 68kDa. Antibodies to NF-L are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. Mutations in the protein coding region of the human NF-L gene cause some forms of Charcot-Marie-Tooth disease (2).

Applications Tested/Suitable for anti-NEFL antibody

Western Blot (WB), Immunofluorescence (IF)

Application Notes for anti-NEFL antibody

Quality Control: Western blots performed on each lot.
WB: 1:10,000
IF: 1:5,000

Product References and Citations for anti-NEFL antibody

• Harris, J., Ayyub, C. and Shaw G. (1991) A molecular dissection of the carboxyterminal tails of the major neurofilament subunits NF-M and NF-H. J Neurosci Res 30:47-62.
• Mersiyanova IV, Perepelov AV, Polyakov AV, Sitnikov VF, Dadali EL, Oparin RB, Petrin AN and Evgrafov OV. (2000) A new variant of Charcot-Marie-Tooth disease type 2 is probably the result of a mutation in the neurofilament-light gene. Am. J. Hum. Genet. 67:37-46.

Other Notes

Small volumes of anti-NEFL antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for NEFL. It may not necessarily be applicable to this product.

NCBI GI #

27806523

NCBI GeneID

281348

NCBI Accession #

NP_776546.1

UniProt Primary Accession #

P02548

UniProt Secondary Accession #

P79127; Q17QQ0

UniProt Related Accession #

P02548

GenBank Nucleotide #

NM_174121.1

NCBI Official Full Name

neurofilament light polypeptide

NCBI Official Synonym Full Names

neurofilament, light polypeptide

NCBI Official Symbol

NEFL

UniProt Protein Name

Neurofilament light polypeptide

UniProt Synonym Protein Names

68 kDa neurofilament protein; Micro glutamic acid-rich protein; Neurofilament triplet L protein

Protein Family

Neurofilament light polypeptide

UniProt Gene Name

NEFL

UniProt Synonym Gene Names

NF-L

UniProt Entry Name

NFL_BOVIN

NCBI Protein Information

neurofilament light polypeptide; NF-L; 68 kDa neurofilament protein; neurofilament triplet L protein; micro glutamic acid-rich protein; neurofilament, light polypeptide 68kDa

UniProt Comments for NEFL

Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Subunit structure: Interacts with ARHGEF28

By similarity. Interacts with TRIM2

By similarity.

Domain: The extra mass and high charge density that distinguish the neurofilament proteins from all other intermediate filament proteins are due to the tailpiece extensions. This region may form a charged scaffolding structure suitable for interaction with other neuronal components or ions.

Post-translational modification: O-glycosylated

By similarity.Phosphorylated in the head and rod regions by the PKC kinase PKN1, leading to the inhibition of polymerization

By similarity. Ref.4Ubiquitinated in the presence of TRIM2 and UBE2D1

By similarity.

Miscellaneous: NF-L is the most abundant of the three neurofilament proteins and, like the other nonepithelial intermediate filament proteins, it can form homopolymeric 10-nm filaments.

Sequence similarities: Belongs to the intermediate filament family.

Mass spectrometry: Molecular mass is 62600 Da from positions 2 - 555. Determined by MALDI. Ref.4

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-NEFL antibody

Products by Pathway	Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram
Amyotrophic Lateral Sclerosis (ALS) Pathway antibodies	Amyotrophic Lateral Sclerosis (ALS) Pathway Diagram

Diseases associated with anti-NEFL antibody

Disease Name	Pubmed Publications
Nervous System Diseases Antibodies	>50 publications with NEFL and Nervous System Diseases
Peripheral Nervous System Diseases Antibodies	>43 publications with NEFL and Peripheral Nervous System Diseases
Polyneuropathies Antibodies	>41 publications with NEFL and Polyneuropathies
Nervous System Malformations Antibodies	>41 publications with NEFL and Nervous System Malformations
Neoplasms Antibodies	>30 publications with NEFL and Neoplasms
Breast Neoplasms Antibodies	>7 publications with NEFL and Breast Neoplasms
Brain Diseases Antibodies	>5 publications with NEFL and Brain Diseases
Charcot-Marie-Tooth disease, Type 1F Antibodies	>4 publications with NEFL and Charcot-Marie-Tooth disease, Type 1F
Pain Antibodies	>3 publications with NEFL and Pain
Lung Neoplasms Antibodies	>2 publications with NEFL and Lung Neoplasms

Organs/Tissues associated with anti-NEFL antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>16 publications with NEFL and Brain
Kidney Antibodies	>2 publications with NEFL and Kidney