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Calretinin (CALB2) Antibody – Sheep Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502148

MBS502148 is an antibody targeting CALB2. Also known as Anti-Calretinin. Reactive in Rat. Host: Sheep. Sheep polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB
Product Documentation
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
Calretinin (CALB2), Polyclonal Antibody
Full Product Name
Anti-Calretinin
Product Gene Name
Clonality
Polyclonal
Host
Sheep
Species Reactivity
Rat
Form/Format
100 ul in 10 mM Tris, 100 mM NaCl.
Purity/Purification
Protein A purified (Protein A purified sheep serum.)
Specificity
Specific for the ~29 kDa calretinin protein in Western blots of rat brain lysate. The antibody also works well for Immunohistochemistry on formalin or paraformaldehyde perfused rat brain tissue sections.
Preparation and Storage
Store at -20 degree C in undiluted aliquots; stable for at least one year after date of receipt. Avoid freeze/thaw cycles.
Molecular Weight
29
Chromosome Location
Chromosome: 19; NC_005118.3 (52310641..52337700). Location: 19q12
3D Structure
ModBase 3D Structure for P47728
Antigen
Purified guinea pig calretinin.
Immunogen Information
Purified guinea pig calretinin
Immunogen Species
Guinea pig
Reactivity Assumed Based on 100% Sequence Homology
Human, mouse, non-human primates
Species Reactivity Note
The antibody has been directly tested for reactivity in rat. It is expected that this antibody will also react with human, mouse and non-human primates.
Biological Significance
Calretinin is a calcium-binding protein involved in calcium signaling. It is abundantly expressed throughout the central nervous system, retina and many other tissues. Studies have shown that calretinin may play a role in neuroprotection by buffering intracellular calcium (Lukas and Jones 1994, Weisenhorn et al., 1996). Additionally, calretinin selectively stains mesothelial cells thus making it an important marker for differentiating malignant mesothelioma from carcinomas (Attanoos et al., 2000).
Applications Tested/Suitable for anti-CALB2 antibody
Western Blot (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes for anti-CALB2 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1,000
IHC: 1:500
IF: 1:500

Testing Data of anti-CALB2 antibody

Sheep Anti-CALB2 Polyclonal Antibody – Cat# MBS502148

Product References and Citations for anti-CALB2 antibody

• Attanoos RL, Dojcinov SD, Webb R, Gibbs AR. (2000) Anti-mesothelial markers in sarcomatoid mesothelioma and other spindle cell neoplasms. Histopathology.37(3):224-31.
• Isaacs KR, Winsky L, Strauss KI, Jacobowitz DM. (1995) Quadruple colocalization of calretinin, calcitonin gene-related peptide, vasoactive intestinal peptide, and substance P in fibers within the villi of the rat intestine. Cell Tissue Res., 280(3):639-51.
• Lukas W, Jones KA (1994) Cortical neurons containing calretinin are selectively resistant to calcium overload and excitotoxicity in vitro. Neurosci. 61(2):307-16.
• Weisenhorn DMV, Weruaga-Prieto E, Celio MR (1996) Calretinin immunoreactivity in organotypic cultures of the rat cerebral cortex: effects of serum deprivation. Exp. Brain Res. 108(1):101-112.

Other Notes

Small volumes of anti-CALB2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for CALB2. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
calretinin
NCBI Official Synonym Full Names
calbindin 2
NCBI Official Symbol
Calb2
UniProt Protein Name
Calretinin
Protein Family
UniProt Gene Name
Calb2
UniProt Synonym Gene Names
CR
UniProt Entry Name
CALB2_RAT
NCBI Protein Information
calretinin; CR

NCBI Summary for CALB2

displays increased mRNA expression following trimethyltin-induced neurodegeneration [RGD, Feb 2006]

UniProt Comments for CALB2

Function: Calretinin is a calcium-binding protein which is abundant in auditory neurons.

Sequence similarities: Belongs to the calbindin family.Contains 6 EF-hand domains.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

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