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EEF1A2 Antibody – Rabbit Polyclonal

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502182

MBS502182 is an antibody targeting EEF1A2. Also known as Anti-Phospho-Ser358 Eukaryotic Elongation Factor 1 alpha 2 (eEF1A2). Reactive in Mouse. Host: Rabbit. Affinity purified rabbit polyclonal antibody More details

$495 / 0.1 mL +1 FREE 8GB USB
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Sample Manual Insert View PDF
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Testing Data View PDF
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Technical Datasheet View PDF
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Product Overview

Online Datasheet in PDF
Product Name
eEF1A2 (Ser358), Polyclonal Antibody
Full Product Name
Anti-Phospho-Ser358 Eukaryotic Elongation Factor 1 alpha 2 (eEF1A2)
Product Gene Name
anti-EEF1A2 antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Mouse
Form/Format
100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots
Purity/Purification
Affinity Purified (Prepared from rabbit serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)
Specificity
Specific for the ~50k eEF1A2 phosphorylated at Ser358. Immunolabeling of the eEF1A2 band is blocked by the treatment of the lysate with lambda phosphatase (30 minutes, 800 units / 1 mg protein).
Preparation and Storage
Store at -20 degree C; stable for at least one year.
Molecular Weight
50
Chromosome Location
Chromosome: 20; NC_000020.11 (63488012..63499315, complement). Location: 20q13.3
OMIM
602959
3D Structure
ModBase 3D Structure for Q05639
Antigen
Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser358 of human eEF1A2.
Immunogen Information
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser358 conjugated to KLH
Immunogen Species
Human
Reactivity Assumed Based on 100% Sequence Homology
Chicken, human, non-human primate, rat, sheep and Xenopus
Species Reactivity Note
The antibody has been directly tested for reactivity in Western blots with mouse tissue. It is anticipated that the antibody will react with chicken, human, non-human primate, rat, sheep, and Xenopus tissues based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
Biological Significance
Eukaryotic Elongation Factor eEF1A exists in two variant forms, eEF1A1 and eEF1A2. While eEF1A1 is almost ubiquitously expressed in humans, eEF1A2 is predominantly found in heart, brain, and skeletal muscle (Knudsen et al., 1993). Expression of eEF1A2 may have a role in ovarian cancer, as its expression is drastically increased in human ovarian tumors (Anand et al., 2002). Due to differences in structural models between the two isoforms, eEF1A1 and eEF1A2 likely have variant-specific phosphorylation sites (Soares et al., 2009). Ribosome-associated JNK phosphorylates Ser358 on eEF1A2 to promote degradation of newly synthesized polypeptides by the proteasome (Gandin et al., 2013).
Applications Tested/Suitable for anti-EEF1A2 antibody
Western Blot (WB)
Application Notes for anti-EEF1A2 antibody
Quality Control: Western blots performed on each lot.
WB: 1:1000

Testing Data of anti-EEF1A2 antibody

Rabbit Anti-EEF1A2 Polyclonal Antibody – Cat# MBS502182

Product References and Citations for anti-EEF1A2 antibody

• Knudsen SM, Frydenberg J, Clark BF, Leffers H. Tissue-dependent variation in the expression of elongation factor-1 alpha isoforms: isolation and characterization of a cDNA encoding a novel variant of human elongation-factor 1 alpha. Eur J Biochem. 1993;215:549-554.
• Anand N, Murphy s, Amann G, Wernick M, Porter LA, Cukier IH, Collins C, Gray JW, Diebold J, Demetrick DJ. Protein elongation factor EEF1A2 is a putative oncogene in ovarian cancer. Nat Genet. 2002;31:301- 305.
• Soares DC, Barlow PN, Newbery HJ, Porteous DJ, Abbot CM. Structural models of human eEF1A1 and eEF1A2 reveal two distant surface clusters of sequence variation and potential differences in phosphorylation. PLoS One. 2009 Jul 28;4(7):e6315.
• Gandin V, Gutierrez GJ, Brill LM, Varsano T, Feng Y, Aza-Blanc P, Au Q, McLaughlan S, Ferreira TA, Alain T, Sonenberg N, Topisirovic I, Ronai ZA. Degradation of newly synthesized polypeptides by ribosomeassociated RACK1/c-Jun N-Terminal Kinase/Eukaryotic Elongation Factor 1A2 complex. 2013 Jul;33(13):2510-26.

Other Notes

Small volumes of anti-EEF1A2 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Additional Details

NCBI/Uniprot data below describe general gene information for EEF1A2. It may not necessarily be applicable to this product.

NCBI GI #
4503475
NCBI GeneID
1917
NCBI Accession #
NP_001949.1
UniProt Primary Accession #
Q05639
UniProt Secondary Accession #
P54266; Q0VGC7; B5BUF3; E1P5J1
UniProt Related Accession #
Q05639
GenBank Nucleotide #
NM_001958.3
NCBI Official Full Name
elongation factor 1-alpha 2
NCBI Official Synonym Full Names
eukaryotic translation elongation factor 1 alpha 2
NCBI Official Symbol
EEF1A2
NCBI Official Synonym Symbols
HS1; STN; EF1A; STNL; EEF1AL; EF-1-alpha-2
UniProt Protein Name
Elongation factor 1-alpha 2
UniProt Synonym Protein Names
Eukaryotic elongation factor 1 A-2; eEF1A-2; Statin-S1
UniProt Gene Name
EEF1A2
UniProt Synonym Gene Names
EEF1AL; STN; EF-1-alpha-2; eEF1A-2
UniProt Entry Name
EF1A2_HUMAN
NCBI Protein Information
elongation factor 1-alpha 2; eEF1A-2; statin S1; statin-S1; elongation factor-1 alpha; eukaryotic elongation factor 1 A-2

NCBI Summary for EEF1A2

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2008]
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UniProt Comments for EEF1A2

Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

Subunit structure: Monomer

By similarity.

Subcellular location: Nucleus

By similarity.

Tissue specificity: Brain, heart, and skeletal muscle.

Sequence similarities: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily.
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Research Articles on EEF1A2

1. results provide evidence of eEF1A2 as a potential therapeutic target in the treatment of aggressive pancreatic cancer

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Pathways associated with anti-EEF1A2 antibody

 Products by Pathway  Pathway Diagram
 Eukaryotic Translation Elongation Pathway antibodies  Eukaryotic Translation Elongation Pathway Diagram
 Gene Expression Pathway antibodies  Gene Expression Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Legionellosis Pathway antibodies  Legionellosis Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 RNA Transport Pathway antibodies  RNA Transport Pathway Diagram
 RNA Transport Pathway antibodies  RNA Transport Pathway Diagram
 Translation Pathway antibodies  Translation Pathway Diagram
 Translation Factors Pathway antibodies  Translation Factors Pathway Diagram

Diseases associated with anti-EEF1A2 antibody

 Disease Name  Pubmed Publications
 Neoplasms Antibodies  >49 publications with EEF1A2 and Neoplasms
 Carcinoma Antibodies  >21 publications with EEF1A2 and Carcinoma
 Adenocarcinoma Antibodies  >14 publications with EEF1A2 and Adenocarcinoma
 Breast Neoplasms Antibodies  >14 publications with EEF1A2 and Breast Neoplasms
 Skin Diseases Antibodies  >12 publications with EEF1A2 and Skin Diseases
 Ovarian Neoplasms Antibodies  >12 publications with EEF1A2 and Ovarian Neoplasms
 Nervous System Diseases Antibodies  >9 publications with EEF1A2 and Nervous System Diseases
 Brain Diseases Antibodies  >8 publications with EEF1A2 and Brain Diseases
 Death Antibodies  >7 publications with EEF1A2 and Death
 Cell Transformation, Neoplastic Antibodies  >7 publications with EEF1A2 and Cell Transformation, Neoplastic

Organs/Tissues associated with anti-EEF1A2 antibody

 Organ/Tissue Name  Pubmed Publications
 Muscle Antibodies  >27 publications with EEF1A2 and Muscle
 Brain Antibodies  >20 publications with EEF1A2 and Brain
 Blood Antibodies  >11 publications with EEF1A2 and Blood
 Heart Antibodies  >7 publications with EEF1A2 and Heart
 Lung Antibodies  >7 publications with EEF1A2 and Lung
 Liver Antibodies  >7 publications with EEF1A2 and Liver
 Intestine Antibodies  >6 publications with EEF1A2 and Intestine
 Nerve Antibodies  >6 publications with EEF1A2 and Nerve
 Prostate Antibodies  >3 publications with EEF1A2 and Prostate
 Thyroid Antibodies  >3 publications with EEF1A2 and Thyroid
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