Rabbit Anti-FMR1 Polyclonal Antibody – Cat# MBS502189

FMRP (Ser499) (FMR1) Antibody – Rabbit Polyclonal

Name: FMR1 Antibody; Polyclonal; Rabbit; MyBioSource
Brand: MyBioSource
SKU: MBS502189
Price: 495 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS502189

Affinity purified rabbit polyclonal antibodyFragile X Mental Retardation Protein (FMRP) is an RNA-binding protein that plays an essential role in cognitive brain function. Mutations in the FMR1 gene, which codes for FMRP, can result in fragile X syndrome,… More details

$495 / 0.1 mL +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

Product Overview

Online Datasheet in PDF

Product Name

FMRP (Ser499) (FMR1), Polyclonal Antibody

Full Product Name

Anti-Phospho-Ser499 FMRP (Fragile X Mental Retardation Protein)

Product Gene Name

anti-FMR1 antibody

Clonality

Polyclonal

Host

Rabbit

Form/Format

100 ul in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 ug per ml BSA and 50% glycerol. Adequate amount of material to conduct 10-mini Western Blots

Purity/Purification

Affinity Purified

Specificity

Specific for ~71k FMRP protein phosphorylated at Ser⁴⁹⁹. Immunolabeling of the FMRP protein is completely eliminated by lambda-phosphatase.

Preparation and Storage

For long term storage -80°C is recommended, but shorter term storage at -20°C is also acceptable as aliquots may be taken without freeze/thawing due to the presence of 50% glycerol. Stock solutions are stable for a minimum of 1 year at -20°C.

Chromosome Location

Chromosome: 1; NC_005100.3 (150408220..150445658, complement). Location: chromosome: 1

3D Structure

ModBase 3D Structure for Q80WE1

Applications Tested/Suitable for anti-FMR1 antibody

Western Blot (WB) and Dot blots (DB). Immunohistochemistry (IHC) has not yet been determined.

Application Notes for anti-FMR1 antibody

Quality Control: Western blots performed on each lot.
WB: 1:1000
DB: 1:1000

Product References and Citations for anti-FMR1 antibody

• Bernard PB, Castano AM, O'Leary H, Simpson K, Browning MD, Benke TA. (2013) Phosphorylation of FMRP and alterations of FMRP complex underlie enhanced mLTD in adult rats triggered by early life seizures. Neurobiol Dis. Nov; 59:1-17.
• Brown V, Small K, Lakkis L, Feng Y, Gunter C, Wilkinson KD, Warren ST (1998) Purified recombinant Fmrp exhibits selective RNA binding as an intrinsic property of the fragile X mental retardation protein. J Biol Chem 273:15521-15527
• Ceman S, O'Donnell WT, Reed M, Patton S, Pohl J, Warren ST. (2003) Phosphorylation influences the translation state of FMRP-associated polyribosomes. Hum Mol Genet. Dec 15;12(24):3295-305
• Goodlin-Jones BL, Tassone F, Gane LW, Hagerman RJ. (2004) Autistic spectrum disorder and the fragile X premutation. J Dev Behav Pediatr. Dec;25(6):392-8
• Narayanan U, Nalavadi V, Nakamoto M, Thomas G, Ceman S, Bassell GJ, Warren ST. (2008) S6K1 phosphorylates and regulates fragile X mental retardation protein (FMRP) with the neuronal protein synthesis-dependent mammalian target of rapamycin (mTOR) signaling cascade. J Biol Chem 283:18478-18482

Other Notes

Small volumes of anti-FMR1 antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for FMR1 purchase

MBS502189 is an antibody targeting FMR1. Also known as Anti-Phospho-Ser499 FMRP (Fragile X Mental Retardation Protein). Host: Rabbit.

Additional Details

NCBI/Uniprot data below describe general gene information for FMR1. It may not necessarily be applicable to this product.

NCBI GI #

30794228

NCBI GeneID

24948

NCBI Accession #

NP_434691.1

UniProt Primary Accession #

Q80WE1

UniProt Secondary Accession #

P70568

UniProt Related Accession #

Q80WE1

GenBank Nucleotide #

NM_052804.1

NCBI Official Full Name

fragile X mental retardation protein 1 homolog

NCBI Official Synonym Full Names

fragile X mental retardation 1

NCBI Official Symbol

Fmr1

NCBI Official Synonym Symbols

FMRP

UniProt Protein Name

Fragile X mental retardation protein 1 homolog

Protein Family

Mat- sexual cell fertilization-promoting factor

UniProt Gene Name

Fmr1

UniProt Synonym Gene Names

FMRP

UniProt Entry Name

FMR1_RAT

NCBI Protein Information

fragile X mental retardation protein 1 homolog; protein FMR-1; fragile X mental retardation-1 protein; fragile X mental retardation syndrome 1 homolog

NCBI Summary for FMR1

may play a role in mRNA trafficking and localization [RGD, Feb 2006]

UniProt Comments for FMR1

Function: Translation repressor. Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit mediates translation repression. RNA-binding protein that plays a role in intracellular RNA transport and in the regulation of translation of target mRNAs. Associated with polysomes. Involved in the transport of mRNA from the nucleus to the cytoplasm. Binds strongly to poly(G), binds moderately to poly(U) but shows very little binding to poly(A) or poly(C)

By similarity.

Subunit structure: Homooligomer. Found in a RNP granule complex with IGF2BP1. Directly interacts with SMN and TDRD3. Interacts with the SMN core complex that contains SMN1, GEMIN2/SIP1, DDX20/GEMIN3, GEMIN4, GEMIN5, GEMIN6, GEMIN7, GEMIN8 and STRAP/UNRIP. Interacts with FXR1, FXR2, IGF2BP1, NUFIP1, NUFIP2, MCRS1 and RANBP9. Component of the CYFIP1-EIF4E-FMR1 complex which is composed of CYFIP, EIF4E and FMR1. Interacts with CYFIP1 and CYFIP2. The interaction with brain cytoplasmic RNA 1 (BC1) increases binding affinity for the CYFIP1-EIF4E complex in the brain

By similarity.

Subcellular location: Cytoplasm

By similarity. Nucleus › nucleolus

By similarity.

Domain: The tandem Tudor domains preferentially recognize trimethylated histone peptides

By similarity.

Post-translational modification: Phosphorylated on several serine residues

By similarity.

Miscellaneous: RNA-binding activity is inhibited by RANBP9

By similarity.

Sequence similarities: Belongs to the FMR1 family.Contains 2 Agenet-like domains.Contains 2 KH domains.

Sequence caution: The sequence AAB07073.1 differs from that shown. Reason: N-ter sequencing errors.

Research Articles on FMR1

1. At least four different isoforms of FMRP are associated with all stages of ovarian follicular growth showing expression patterns that differ from those observed in brain and testis.

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with anti-FMR1 antibody

Reference Product	PubMed Publications
Mtor antibody	>22 publications with FMR1 and Mtor
Cyfip1 antibody	>14 publications with FMR1 and Cyfip1
Eif4e antibody	>6 publications with FMR1 and Eif4e
Cyfip2 antibody	>5 publications with FMR1 and Cyfip2
Nxf1 antibody	>2 publications with FMR1 and Nxf1
Eif2c2 antibody	>1 publications with FMR1 and Eif2c2
Ncl antibody	>1 publications with FMR1 and Ncl

Pathways associated with anti-FMR1 antibody

Products by Pathway	Pathway Diagram
RNA Transport Pathway antibodies	RNA Transport Pathway Diagram
RNA Transport Pathway antibodies	RNA Transport Pathway Diagram

Diseases associated with anti-FMR1 antibody

Disease Name	Pubmed Publications
Fragile X Syndrome Antibodies	>1724 publications with FMR1 and Fragile X Syndrome
Nervous System Diseases Antibodies	>1520 publications with FMR1 and Nervous System Diseases
Intellectual Disability Antibodies	>1489 publications with FMR1 and Intellectual Disability
Congenital Abnormalities Antibodies	>1411 publications with FMR1 and Congenital Abnormalities
Primary Ovarian Insufficiency Antibodies	>126 publications with FMR1 and Primary Ovarian Insufficiency
Ovarian Diseases Antibodies	>117 publications with FMR1 and Ovarian Diseases
Autistic Disorder Antibodies	>92 publications with FMR1 and Autistic Disorder
Cognition Disorders Antibodies	>76 publications with FMR1 and Cognition Disorders
Seizures Antibodies	>66 publications with FMR1 and Seizures
Memory Disorders Antibodies	>58 publications with FMR1 and Memory Disorders

Organs/Tissues associated with anti-FMR1 antibody

Organ/Tissue Name	Pubmed Publications
Brain Antibodies	>629 publications with FMR1 and Brain
Embryonic Tissue Antibodies	>55 publications with FMR1 and Embryonic Tissue
Testis Antibodies	>40 publications with FMR1 and Testis
Muscle Antibodies	>33 publications with FMR1 and Muscle
Eye Antibodies	>30 publications with FMR1 and Eye
Kidney Antibodies	>23 publications with FMR1 and Kidney
Liver Antibodies	>14 publications with FMR1 and Liver
Heart Antibodies	>13 publications with FMR1 and Heart
Placenta Antibodies	>9 publications with FMR1 and Placenta
Lung Antibodies	>5 publications with FMR1 and Lung