Vinculin (VCL) ELISA Kit for Canine

Name: Canine VCL ELISA Kit; Vinculin; MyBioSource
Brand: MyBioSource
SKU: MBS005044
Price: 675 USD
Availability: InStock
Rating: 5 (10 reviews)

For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS005044

Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated VCL concentrations in Canine serum, plasma and other body fluids. Using Purified Canine VCL antibody to coat Microelisa Stripplate wells… More details

$675 / 48-Strip-Wells +1 FREE 8GB USB

Product Documentation

Sample Manual Insert View PDF

Testing Data View PDF

Certificate of Analysis (COA) View PDF

QC Report View PDF

Material Safety Data Sheet View PDF

Technical Datasheet View PDF

Protocol View PDF

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Product Overview

Online Datasheet in PDF

Product Name

Vinculin (VCL), ELISA Kit

Full Product Name

Canine Vinculin ELISA Kit

Product Gene Name

VCL elisa kit

Species Reactivity

Canine

Assay Type

Sandwich

Samples

Serum, Plasma, Tissue Homogenate, Feces and Urine

Detection Range

0.625ng/ml-20ng/ml.

Sensitivity

0.1ng/ml.

Specificity

No significant cross-reactivity or interference between Canine VCL and analogues was observed.

Preparation and Storage

Store all reagents at 2-8 degree C

Inter-assay Precision

Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]

Intra-assay Precision

Intra-assay CV (%) is less than 15%.

Molecular Weight

123,799 Da

Chromosome Location

Chromosome: 10; NC_000010.10 (75757872..75879918). Location: 10q22.2

OMIM

193065

3D Structure

ModBase 3D Structure for P18206

Intended Uses

This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated VCL concentrations in Canine serum, plasma and other body fluids. Using Purified Canine VCL antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add VCL and VCL antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of VCL in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Other Notes

Small volumes of VCL elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for VCL purchase

MBS005044 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Vinculin (VCL) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing VCL. The ELISA analytical biochemical technique of the MBS005044 kit is based on VCL antibody-VCL antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect VCL antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, VCL. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for VCL. It may not necessarily be applicable to this product.

NCBI GI #

246714

NCBI GeneID

7414

NCBI Accession #

AAB21657.1

UniProt Primary Accession #

P18206

UniProt Secondary Accession #

Q16450; Q5SWX2; Q7Z3B8; Q8IXU7

UniProt Related Accession #

P18206

NCBI Official Full Name

vinculin

NCBI Official Synonym Full Names

vinculin

NCBI Official Symbol

VCL

NCBI Official Synonym Symbols

MV; MVCL; CMD1W; CMH15

UniProt Protein Name

Vinculin

UniProt Synonym Protein Names

Metavinculin

Protein Family

Vinculin

UniProt Gene Name

VCL

UniProt Synonym Gene Names

UniProt Entry Name

VINC_HUMAN

NCBI Protein Information

vinculin; metavinculin

NCBI Summary for VCL

Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Defects in VCL are the cause of cardiomyopathy dilated type 1W. Dilated cardiomyopathy is a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

UniProt Comments for VCL

Vinculin: Actin filament (F-actin)-binding protein involved in cell-matrix adhesion and cell-cell adhesion. Regulates cell- surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. May also play important roles in cell morphology and locomotion. Exhibits self-association properties. Interacts with NRAP and SORBS1. Interacts with TLN1. Interacts with SYNM. Interacts with CTNNB1 and this interaction is necessary for its localization to the cell-cell junctions and for its function in regulating cell surface expression of E-cadherin. Metavinculin is muscle-specific. Belongs to the vinculin/alpha-catenin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cytoskeletal; Motility/polarity/chemotaxis; Cell adhesion

Chromosomal Location of Human Ortholog: 10q22.2

Cellular Component: focal adhesion; protein complex; costamere; extracellular region; fascia adherens; intercellular junction; cytosol; actin cytoskeleton; cell-matrix junction; adherens junction; cytoskeleton; cell-cell adherens junction; plasma membrane; vesicle

Molecular Function: protein binding; cadherin binding; beta-catenin binding; structural molecule activity; actin binding; alpha-catenin binding

Biological Process: lamellipodium biogenesis; platelet activation; platelet degranulation; apical junction assembly; muscle contraction; axon extension; cell-matrix adhesion; morphogenesis of an epithelium; cell motility; blood coagulation; cell adhesion; negative regulation of cell migration

Disease: Cardiomyopathy, Dilated, 1w

Research Articles on VCL

1. VAX1 rs10787760, rs6585429 and rs1871345 polymorphisms may be involved in nonsyndromic cleft lip with or without cleft palate in Brazilian patients, but there is no association with polymorphisms in FGF12, VCL, or CX43

Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with VCL elisa kit

Reference Product	PubMed Publications
SRC elisa kit	>6 publications with VCL and SRC
CTNNB1 elisa kit	>5 publications with VCL and CTNNB1
PXN elisa kit	>3 publications with VCL and PXN
TPM1 elisa kit	>2 publications with VCL and TPM1
CTNNA1 elisa kit	>2 publications with VCL and CTNNA1

Pathways associated with VCL elisa kit

Products by Pathway	Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Adherens Junction Pathway antibodies	Adherens Junction Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Amoebiasis Pathway antibodies	Amoebiasis Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Bacterial Invasion Of Epithelial Cells Pathway antibodies	Bacterial Invasion Of Epithelial Cells Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Focal Adhesion Pathway antibodies	Focal Adhesion Pathway Diagram
Hemostasis Pathway antibodies	Hemostasis Pathway Diagram

Diseases associated with VCL elisa kit

Disease Name	Pubmed Publications
Cardiovascular Diseases Antibodies	>33 publications with VCL and Cardiovascular Diseases
Neoplasms Antibodies	>26 publications with VCL and Neoplasms
Drug Toxicity Antibodies	>25 publications with VCL and Drug Toxicity
Nervous System Diseases Antibodies	>15 publications with VCL and Nervous System Diseases
Heart Diseases Antibodies	>15 publications with VCL and Heart Diseases
Brain Diseases Antibodies	>12 publications with VCL and Brain Diseases
Kidney Diseases Antibodies	>6 publications with VCL and Kidney Diseases
Neoplasm Metastasis Antibodies	>6 publications with VCL and Neoplasm Metastasis
Poisoning Antibodies	>5 publications with VCL and Poisoning
Lung Diseases Antibodies	>4 publications with VCL and Lung Diseases

Organs/Tissues associated with VCL elisa kit

Organ/Tissue Name	Pubmed Publications
Blood Antibodies	>48 publications with VCL and Blood
Muscle Antibodies	>44 publications with VCL and Muscle
Vascular Antibodies	>20 publications with VCL and Vascular
Heart Antibodies	>17 publications with VCL and Heart
Testis Antibodies	>16 publications with VCL and Testis
Brain Antibodies	>14 publications with VCL and Brain
Lung Antibodies	>12 publications with VCL and Lung
Liver Antibodies	>10 publications with VCL and Liver
Kidney Antibodies	>10 publications with VCL and Kidney
Bone Antibodies	>8 publications with VCL and Bone